DC-SIGN/CD209: Novus Biologicals

MAB161

DC-SIGN/CD209 Antibody (120507) [Unconjugated]

(1 Review)

100 μg

MAB161

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

DC-SIGN was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." alt="DC-SIGN was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

DC-SIGN was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

DC-SIGN was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

Human DC-SIGN and DC-SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_lightbox">

Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # <a class=

FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_thumb" />

<b>Biological Strategies Validation. </b>Infectivity of KSHV is enhanced in the presence of DC-SIGN and DC-SIGNR.A) 293T cells were transfected with empty pcDNA3 vector or expression constructs for DC-SIGN or DC-SIGNR. After 24 hours, cells were infected with 20 µl Bac16 delta K3 delta K5 or left uninfected as controls. Cells were harvested after additional 24 hours and surface stained with a DC-SIGN/R antibody (H-200) and analyzed by flow cytometry. Top three panels show transfected cells stained for DC-SIGN/R followed by PE- (DC-SIGN), FITC- (DC-SIGNR) or both (vector) conjugated secondary antibodies. Bottom panels shows KSHV infection of 293T cells transiently expressing DC-SIGN or DC-SIGNR. B, left panels) 293 cell lines stably expressing a vector construct, DC-SIGN or DC-SIGNR were fluorescently stained for surface expression of DC-SIGN or DC-SIGNR. The mean channel fluorescence is indicated in the upper right hand corner. Open histograms – secondary antibody alone; shaded histograms – DC-SIGN or DC-SIGNR staining. B, right panel) 293 pcDNA3, DC-SIGN or DC-SIGNR stable cell lines were pre-incubated with a control antibody (anti-ICAM1, 7 µg/ml), with mannan (100 µg/ml) or a monoclonal antibody specific for DC-SIGN (MAB161; 7 µg/ml) for 30 minutes on ice. These cells were then infected with wild type KSHV (Bac16 or rKSHV.219) at an MOI of 0.01. After 72 hours cells were harvested and evaluated for infection by flow cytometry measuring GFP expression. Infection rates were normalized to 293 pcDNA3 cells treated with the control antibody. The fold increase in relative infectivity is indicated. Data are representative of four independent experiments with two performed in triplicate. C) 293 pcDNA3, DC-SIGN or DC-SIGNR stable lines were infected with 50 µl of concentrated Bac16 wildtype (wt), or mutants with deletion of K3 only ( delta K3), K5 only ( delta K5), or deletion of both K3 and K5 ( delta K3 delta K5) as indicated. At 72 hours post-infection, the cells were stained for surface expression of DC-SIGN, DC-SIGNR or MHC class I. GFP fluore

Rapid induction and persistence of DC-SIGN, S100 and CD68 post-vaccination in lymphoid tissue.Galleries of representative fields of A DC-SIGN and B CD68 staining in MLN at 3, 7, 10, 21, 125 d.p.i. C Representative fields of DC-SIGN signal tracking through the spleen 3–125 d.p.i. maximal from d3 in red pulp (rp) and marginal zones (mg) D Representative fields of splenic S100 levels illustrate similar staining intensity 3–125 d.p.i. predominantly in the germinal centres and red pulp. Staining intensities scoring were; pale yellow (no staining, −); yellow (very low, +); dark yellow (low, ++), red (medium, +++) and magenta (high, ++++). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25162725), licensed under a CC-BY license. Not internally tested by R&D Systems.

Infection of DC-SIGN+ cells in the lung.(A) Infection of the DC-SIGNhi, DC-SIGNlo and DC-SIGN- cells in BAL from MV-infected macaques, determined by detection of EGFP in flow cytometry at day 2–5 d.p.i. Each dot represents an individual animal. Lines indicate geometric means. (B) Macroscopic images from EGFP+ lung slices collected 3 d.p.i., cultured for additional 3,5,7 or 10 days. (C) Phenotype of cells migrating from the ex vivo cultured lung slice, collected from supernatant after 5 days of culturing (D) Phenotype of EGFP+ cells collected from lung slice medium. (E-F) DC-SIGN expression on lung sections from uninfected macaques (E) or 2.d.p.i. (F) Asterisks indicate DC-SIGN reactivity. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23227146), licensed under a CC-BY license. Not internally tested by R&D Systems.

K3 and K5 affect the stability of DC-SIGN and DC-SIGNR in a RING-CH domain-dependent mechanism.A) 293 cells stably expressing wild-type K3 or K5, or the RING-CH mutant of either viral protein were transiently transfected with 2 µg DC-SIGN or DC-SIGNR constructs. At ∼48 hpt,cells were lysed in RIPA buffer and 30 µg of normalized lysate were loaded per sample. Protein levels of DC-SIGN or DC-SIGNR were determined by WB, and then blots were reprobed for lamin B as a loading control. Data is representative of at least three independent experiments. B) THP-1 cell stably expressing the indicated K5 constructs or empty vector were stained for cell surface levels of DC-SIGN. Solid histogram, empty vector; grey histogram, K5 construct; dotted histogram, isotype control. C) The same THP-1 cell lines used in Panel B, were lysed and subjected to western blotting with either a DC-SIGN (H-200) or GAPDH (O411) antibody, as loading control. Data is representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23460925), licensed under a CC-BY license. Not internally tested by R&D Systems.

DC-SIGN is expressed by DCs and a subset of macrophages in lymph nodes.(A) TBLN cells were stained for DC-SIGN in combination with DC and macrophage markers and analyzed by flow cytometry. Gray areas show negative controls (DC-SIGN single staining). Percentages of positive cells expressing the markers are annotated in the upper right corner. (B) DC-SIGN expression of CD11c+ and CD83+ cells in TBLNs. (C-D) Dual immunofluorescence staining of DC-SIGN (green) and MAC387 (red) in lung sections 2 or 3 d.p.i. (C i-iv) and axillary lymphoid tissue 4 d.p.i. (D). Nuclei are stained blue with Hoechst. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23227146), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB161U-100UG
Alexa Fluor 405	FAB161V-100UG
Alexa Fluor 488	FAB161G-100UG
Alexa Fluor 532	FAB161X
Alexa Fluor 594	FAB161T-100UG
Alexa Fluor 647	FAB161R-100UG
Alexa Fluor 700	FAB161N-100UG
Alexa Fluor 750	FAB161S-100UG
Alexa Fluor Plus 405	FAB161AFP405-100UG
Alexa Fluor Plus 488	FAB161AFP488-100UG
Alexa Fluor Plus 555	FAB161AFP555-100UG
Alexa Fluor Plus 594	FAB161AFP594-100UG
Alexa Fluor Plus 647	FAB161AFP647-100UG
Alexa Fluor Plus 680	FAB161AFP680-100UG
Allophycocyanin	FAB161A-025
Allophycocyanin	FAB161A-100
Allophycocyanin/Cy7	FAB161APCCY7
Biotin	FAB161B
CoraFluor 1	FAB161CL1
DyLight 350	FAB161D
DyLight 405	FAB161E
DyLight 488	FAB161K
DyLight 550	FAB161L
DyLight 594	FAB161M
DyLight 650	FAB161W
DyLight 680	FAB161Y
DyLight 755	FAB161Z
Fluorescein	FAB161F-025
Fluorescein	FAB161F-100
HRP	FAB161H
Janelia Fluor 525	FAB161JF525
Janelia Fluor 549	FAB161I
Janelia Fluor 646	FAB161J
Janelia Fluor 669	FAB161JF669
PE/Atto594	FAB161PEATT594
PE/Cy5.5	FAB161PECY55
PE/Cy7	FAB161PECY7
Phycoerythrin	FAB161P-100
Phycoerythrin	FAB161P-025
mFluor Violet 450 SE	FAB161MFV450
mFluor Violet 500 SE	FAB161MFV500
mFluor Violet 610 SE	FAB161MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB161N-100UG
Carrier Free	MAB161-SP
Carrier Free	MAB161-500
Carrier Free	FAB161P-100
Carrier Free	FAB161P-025
Carrier Free	FAB161F-100
Carrier Free	FAB161F-025
Carrier Free	FAB161A-100
Carrier Free	FAB161A-025
Carrier Free	FAB161V-100UG
Carrier Free	FAB161U-100UG
Carrier Free	FAB161T-100UG
Carrier Free	FAB161S-100UG
Carrier Free	FAB161R-100UG
Carrier Free	FAB161G-100UG

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MAB1621

DC-SIGN+DC-SIGNR Antibody (120612)

(17 Publications)

100 μg

MAB1621

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

Human DC-SIGN and DC-SIGNR transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN+ DC-SIGNR Monoclonal Antibody (Catalog # MAB1621, filled histograms) or isotype control antibody (Catalog # <a class=

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="Human DC-SIGN and DC-SIGNR transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN+ DC-SIGNR Monoclonal Antibody (Catalog # MAB1621, filled histograms) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

FAB141F).Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003)." class="big_lightbox">

Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN+ DC-SIGNR Monoclonal Antibody (Catalog # MAB1621), followed by PE-conjugated anti-mouse secondary antibody (Catalog # F0102B) and Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # <a class=

FAB141F).Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003)." class="big_thumb" />

CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

DC-SIGN+DC-SIGNR was detected in immersion fixed paraffin-embedded sections of human lymphoma using 25 µg/mL Mouse Anti-Human DC-SIGN+ DC-SIGNR Monoclonal Antibody (Catalog # MAB1621) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

The C type lectin DC-SIGN enhances infection of human cells by tick cell-derived rUUKV S23. (A) BHK-21 cells were infected (at an MOI of 0.1) with rUUKV S23 derived from IRE/CTVM19 cells for 18 h and immunostained for N, GN, and GC proteins prior to flow cytometry analysis. (B) Parental (Raji) and DC-SIGN-expressing Raji cells (Raji DC-SIGN+) were infected with IRE/CTVM19 cell-derived rUUKV S23 and analyzed by flow cytometry 16 h after immunostaining against the viral nucleoprotein. (C) Parental (HeLa) and DC-SIGN-expressing HeLa cells (HeLa DC-SIGN+) were exposed to various MOIs of IRE/CTVM19 cell-derived rUUKV S23. The next day, infected cells were immunostained for the intracellular virus nucleoprotein N using the anti-N primary mouse monoclonal antibody 8B11A3 and an AF488-coupled anti-mouse secondary monoclonal antibody (green). Nuclei were stained with Hoechst (blue), and samples were analyzed by wide-field microscopy. (D) Raji DC-SIGN-expressing cells were exposed to IRE/CTVM19 cell-derived rUUKV S23 (MOI of ∼1) in the presence of inhibitors blocking DC-SIGN, namely, EDTA (5 mM) or the neutralizing mouse monoclonal antibody mAb1621 (25 μg · ml−1). Intracellular viral antigens were detected by immunostaining with an anti-UUKV rabbit polyclonal antibody, followed by incubation with AF647-conjugated secondary antibodies. Infection was analyzed by flow cytometry 18 h later and normalized to infection of DC-SIGN-expressing Raji cells in the absence of inhibitor (as a percentage of the control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27194760), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1621U-100UG
Alexa Fluor 405	FAB1621V-100UG
Alexa Fluor 488	FAB1621G-100UG
Alexa Fluor 532	FAB1621X
Alexa Fluor 594	FAB1621T-100UG
Alexa Fluor 647	FAB1621R-100UG
Alexa Fluor 700	FAB1621N-100UG
Alexa Fluor 750	FAB1621S-100UG
Alexa Fluor Plus 405	FAB1621AFP405-100UG
Alexa Fluor Plus 488	FAB1621AFP488-100UG
Alexa Fluor Plus 555	FAB1621AFP555-100UG
Alexa Fluor Plus 594	FAB1621AFP594-100UG
Alexa Fluor Plus 647	FAB1621AFP647-100UG
Alexa Fluor Plus 680	FAB1621AFP680-100UG
Allophycocyanin	FAB1621A
Allophycocyanin/Cy7	FAB1621APCCY7
Biotin	FAB1621B
CoraFluor 1	FAB1621CL1
DyLight 350	FAB1621D
DyLight 405	FAB1621E
DyLight 488	FAB1621K
DyLight 550	FAB1621L
DyLight 594	FAB1621M
DyLight 650	FAB1621W
DyLight 680	FAB1621Y
DyLight 755	FAB1621Z
HRP	FAB1621H
Janelia Fluor 525	FAB1621JF525
Janelia Fluor 549	FAB1621I
Janelia Fluor 646	FAB1621J
Janelia Fluor 669	FAB1621JF669
PE/Atto594	FAB1621PEATT594
PE/Cy5.5	FAB1621PECY55
PE/Cy7	FAB1621PECY7
PerCP	FAB1621C
Phycoerythrin	FAB1621P
mFluor Violet 450 SE	FAB1621MFV450
mFluor Violet 500 SE	FAB1621MFV500
mFluor Violet 610 SE	FAB1621MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1621P
Carrier Free	MAB1621-SP
Carrier Free	MAB1621-500
Carrier Free	FAB1621V-100UG
Carrier Free	FAB1621U-100UG
Carrier Free	FAB1621T-100UG
Carrier Free	FAB1621S-100UG
Carrier Free	FAB1621R-100UG
Carrier Free	FAB1621N-100UG
Carrier Free	FAB1621G-100UG

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9136-DC

Recombinant Human DC-SIGN/CD209 Protein, CF

(2 Publications)

50 μg

9136-DC

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DDX0207

DC-SIGN/CD209 Antibody (109H12.03) - Azide and BSA Free

(8 Publications)

0.1 mg

DDX0207

Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis of Alexa Fluor (R) 647 conjugate of DDX0207. FACS staining of CD14+ CD209+ cells. (CD14+ cells cultured 5 days with GM CSF and IL-10)

Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0207. A) Staining of DC-SIGN transfected HeLa cells. B) IF staining of DC-SIGN transfected cells with 109H12.03.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0207A488
Alexa Fluor 546	DDX0207A546
Alexa Fluor 647	DDX0207A647
Biotin	DDX0207B

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MAB16211

DC-SIGN+DC-SIGNR Antibody (DC28)

(7 Publications)

500 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB16211U-100UG
Alexa Fluor 405	FAB16211V-100UG
Alexa Fluor 488	FAB16211G-100UG
Alexa Fluor 532	FAB16211X
Alexa Fluor 594	FAB16211T-100UG
Alexa Fluor 647	FAB16211R-100UG
Alexa Fluor 700	FAB16211N-100UG
Alexa Fluor 750	FAB16211S-100UG
Alexa Fluor Plus 405	FAB16211AFP405-100UG
Alexa Fluor Plus 488	FAB16211AFP488-100UG
Alexa Fluor Plus 555	FAB16211AFP555-100UG
Alexa Fluor Plus 594	FAB16211AFP594-100UG
Alexa Fluor Plus 647	FAB16211AFP647-100UG
Alexa Fluor Plus 680	FAB16211AFP680-100UG
Allophycocyanin	FAB16211A
Allophycocyanin/Cy7	FAB16211APCCY7
Biotin	FAB16211B
DyLight 350	FAB16211D
DyLight 405	FAB16211E
DyLight 488	FAB16211K
DyLight 550	FAB16211L
DyLight 594	FAB16211M
DyLight 650	FAB16211W
DyLight 680	FAB16211Y
DyLight 755	FAB16211Z
FITC	FAB16211F
HRP	FAB16211H
Janelia Fluor 525	FAB16211JF525
Janelia Fluor 549	FAB16211I
Janelia Fluor 646	FAB16211J
Janelia Fluor 669	FAB16211JF669
PE	FAB16211P
PE/Atto594	FAB16211PEATT594
PE/Cy5.5	FAB16211PECY55
PE/Cy7	FAB16211PECY7
PerCP	FAB16211C

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB16211-SP
Carrier Free	FAB16211V-100UG
Carrier Free	FAB16211U-100UG
Carrier Free	FAB16211T-100UG
Carrier Free	FAB16211S-100UG
Carrier Free	FAB16211R-100UG
Carrier Free	FAB16211N-100UG
Carrier Free	FAB16211G-100UG

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DDX0202P-100

DC-SIGN/CD209 Antibody (102E11.06) - Azide and BSA Free

(5 Publications)

0.1 mg

DDX0202P-100

Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (102E11.06) [DDX0202P-100] - Human tonsil frozen section

Flow Cytometry: DC-SIGN/CD209 Antibody (102E11.06) [DDX0202P-100]

Reactivity:

Hu, Mu, Ca

Species Glossary

Reactivity: Hu, Mu, Ca

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0202A488-100
Alexa Fluor 546	DDX0202A546-100
Alexa Fluor 647	DDX0202A647-100
Biotin	DDX0202B-100

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NBP2-27408

DC-SIGN/CD209 Antibody

0.1 mg

NBP2-27408

Reactivity:

Hu, Mu, Pm, Pm, Pm-Or, RM

Species Glossary

Reactivity: Hu, Mu, Pm, Pm, Pm-Or, RM

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6806A

Alt Catalog# IMG-6806A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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DDX0209

DC-SIGN/CD209 Antibody (111H2.02) - Azide and BSA Free

(5 Publications)

0.1 mg

DDX0209

Flow Cytometry: DC-SIGN/CD209 Antibody (111H2.02) [DDX0209] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0209. A) Staining of NIH3T3 transfected cells with 111H2.02. B) Endogenous expression revealed by Flow staining with 111H2.02.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0209A488
Alexa Fluor 647	DDX0209A647
Biotin	DDX0209B

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NBP1-77284

DC-SIGN/CD209 Antibody - BSA Free

0.1 mg

NBP1-77284

Immunohistochemistry: DC-SIGN/CD209 Antibody - BSA Free [NBP1-77284] - Immunohistochemistry of DC-SIGN/CD209 in human lymph node tissue with DC-SIGN/CD209 antibody at 10 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5563

Alt Catalog# IMG-5563

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77284AF350
Alexa Fluor 405	NBP1-77284AF405
Alexa Fluor 488	NBP1-77284AF488
Alexa Fluor 532	NBP1-77284AF532
Alexa Fluor 594	NBP1-77284AF594
Alexa Fluor 647	NBP1-77284AF647
Alexa Fluor 700	NBP1-77284AF700
Alexa Fluor 750	NBP1-77284AF750
Alexa Fluor Plus 405	NBP1-77284AFP405
Alexa Fluor Plus 488	NBP1-77284AFP488
Alexa Fluor Plus 555	NBP1-77284AFP555
Alexa Fluor Plus 594	NBP1-77284AFP594
Alexa Fluor Plus 647	NBP1-77284AFP647
Alexa Fluor Plus 680	NBP1-77284AFP680
Allophycocyanin	NBP1-77284APC
Biotin	NBP1-77284B
CoraFluor 1	NBP1-77284CL1
DyLight 350	NBP1-77284UV
DyLight 405	NBP1-77284V
DyLight 488	NBP1-77284G
DyLight 550	NBP1-77284R
DyLight 594	NBP1-77284DL594
DyLight 650	NBP1-77284C
DyLight 680	NBP1-77284FR
DyLight 755	NBP1-77284IR
FITC	NBP1-77284F
HRP	NBP1-77284H
Janelia Fluor 525	NBP1-77284JF525
Janelia Fluor 549	NBP1-77284JF549
Janelia Fluor 646	NBP1-77284JF646
Janelia Fluor 669	NBP1-77284JF669
PE	NBP1-77284PE
PerCP	NBP1-77284PCP
mFluor Violet 450 SE	NBP1-77284MFV450
mFluor Violet 500 SE	NBP1-77284MFV500
mFluor Violet 610 SE	NBP1-77284MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77284-0.025mg

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DDX0205P-100

DC-SIGN/CD209 Antibody (102F10.04) - Azide and BSA Free

(3 Publications)

0.1 mg

DDX0205P-100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0205A488-100
Alexa Fluor 546	DDX0205A546-100
Alexa Fluor 647	DDX0205A647-100

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NBP2-53275

DC-SIGN/CD209 Antibody (C209/1781)

100 μg

NBP2-53275

Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) [NBP2-53275] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).

Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) [NBP2-53275] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54351AF350
Alexa Fluor 405	NBP2-54351AF405
Alexa Fluor 488	NBP2-54351AF488
Alexa Fluor 532	NBP2-54351AF532
Alexa Fluor 594	NBP2-54351AF594
Alexa Fluor 647	NBP2-54351AF647
Alexa Fluor 700	NBP2-54351AF700
Alexa Fluor 750	NBP2-54351AF750
Alexa Fluor Plus 405	NBP2-54351AFP405
Alexa Fluor Plus 488	NBP2-54351AFP488
Alexa Fluor Plus 555	NBP2-54351AFP555
Alexa Fluor Plus 594	NBP2-54351AFP594
Alexa Fluor Plus 647	NBP2-54351AFP647
Alexa Fluor Plus 680	NBP2-54351AFP680
Allophycocyanin	NBP2-54351APC
Biotin	NBP2-54351B
CoraFluor 1	NBP2-54351CL1
DyLight 350	NBP2-54351UV
DyLight 405	NBP2-54351V
DyLight 488	NBP2-54351G
DyLight 550	NBP2-54351R
DyLight 594	NBP2-54351DL594
DyLight 650	NBP2-54351C
DyLight 680	NBP2-54351FR
DyLight 755	NBP2-54351IR
FITC	NBP2-54351F
HRP	NBP2-54351H
Janelia Fluor 525	NBP2-54351JF525
Janelia Fluor 549	NBP2-54351JF549
Janelia Fluor 646	NBP2-54351JF646
Janelia Fluor 669	NBP2-54351JF669
PE	NBP2-54351PE
mFluor Violet 450 SE	NBP2-54351MFV450
mFluor Violet 500 SE	NBP2-54351MFV500
mFluor Violet 610 SE	NBP2-54351MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54351-100ug

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AF161

DC-SIGN/CD209 Antibody [Unconjugated]

100 μg

AF161

HAF019). A specific band was detected for DC-SIGN/CD209 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human DC-SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for DC-SIGN/CD209 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human DC-SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DC-SIGN/CD209 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human DC‑SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human immature dendritic cells." class="big_lightbox">

<b>Left:</b> Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.4 mg/ml. A specific band was detected for DC‑SIGN/CD209 at approximately 59 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human DC‑SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human DC‑SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human immature dendritic cells." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF161U-100UG
Alexa Fluor 405	AF161V-100UG
Alexa Fluor 488	AF161G-100UG
Alexa Fluor 532	AF161X-100UG
Alexa Fluor 594	AF161T-100UG
Alexa Fluor 647	AF161R-100UG
Alexa Fluor 700	AF161N-100UG
Alexa Fluor 750	AF161S-100UG
Alexa Fluor Plus 405	AF161AFP405-100UG
Alexa Fluor Plus 488	AF161AFP488-100UG
Alexa Fluor Plus 555	AF161AFP555-100UG
Alexa Fluor Plus 594	AF161AFP594-100UG
Alexa Fluor Plus 647	AF161AFP647-100UG
Alexa Fluor Plus 680	AF161AFP680-100UG
Allophycocyanin	AF161A
Biotin	AF161B
CoraFluor 1	AF161CL1
DyLight 350	AF161D
DyLight 405	AF161E
DyLight 488	AF161K
DyLight 550	AF161L
DyLight 594	AF161M
DyLight 650	AF161W
DyLight 680	AF161Y
DyLight 755	AF161Z
FITC	AF161F
HRP	AF161H
Janelia Fluor 525	AF161JF525
Janelia Fluor 549	AF161I
Janelia Fluor 646	AF161J
Janelia Fluor 669	AF161JF669
PE	AF161P
PerCP	AF161C
mFluor Violet 450 SE	AF161MFV450
mFluor Violet 500 SE	AF161MFV500
mFluor Violet 610 SE	AF161MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF161-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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DC-SIGN/CD209 Antibody (102F10.04) - Azide and BSA Free

DC-SIGN/CD209 Antibody (C209/1781)

DC-SIGN/CD209 Antibody [Unconjugated]