| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88231-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03014-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06657-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06657-100ul | |||
| Azide and BSA Free | NBP3-06657-50ul | |||
![Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP1-28751-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Polyclonal GW182 Antibody [NBP1-28751] - Detection of human GW182 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibodyNBP1-28751 (lot NBP1-28751-2) used for IP at 6 ug/mg lysate. GW182 was also immunoprecipitated by a previous lot (lot NBP1-28751-1) of this antibody. For blotting immunoprecipitated GW182, NBP1-28751 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-NBP1-28751-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GW182 Antibody [NBP1-28751] - Downregulation of eIF4E2 increases the protein levels of endogenous IMP1. HeLa cells were transfected with siRNAs indicated. At 48 h posttransfection, cells were lysed. A fraction of the lysate was subjected to SDS-PAGE followed by Western blotting. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s13238-017-0444-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GW182-Antibody-Knockdown-Validated-NBP1-28751-img0005.jpg)
![Western Blot: GW182 Antibody [NBP1-28751] - TNRC6A interacts with eIF4E2. (A) Plasmids expressing proteins indicated were transfected into HEK293T cells. At 48 h posttransfection, cells were lysed & the lysates were immunoprecipitated (IP) in the presence of RNase A. The precipitates were resolved on SDS-PAGE followed by Western blotting. (B) A plasmid expressing Flag-tagged eIF4E1 or eIF4E2 was transfected into HEK293T cells. At 48 h posttransfection, cells were lysed & the lysates were immunoprecipitated with the anti-TNRC6A antibody or control IgG in the presence of RNase A. The precipitates were resolved on SDS-PAGE followed by Western blotting. (C) Upper: schematic representation of TNRC6A truncation mutants. Lower: A plasmid expressing the TNRC6A mutant indicated & a plasmid expressing myc-tagged eIF4E2 were transiently transfected into HEK293T cells. At 48 h posttransfection, cells were lysed & the lysates were immunoprecipitated with anti-Flag antibody in the presence of RNase A followed by Western blotting. (D) Bacterially expressed Flag-tagged eIF4E2 or eIF4E1 was incubated with Glutathione Sepharose 4B bound fusion protein of GST & the C-terminal domain of TNRC6A (6A-C). The precipitates were washed & resolved on SDS-PAGE followed by commassie brilliant blue staining (lower) & Western blotting (upper) Image collected & cropped by CiteAb from the following publication (//academic.oup.com/proteincell/article/8/10/750/6765124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28751_rabbit-polyclonal-gw182-antibody-310202416163743.jpg)
![Western Blot: GW182 Antibody [NBP1-28751] - Cellular expression of TBG nanocapsule uptake & RNAi-CK2 oligomer processing markers in xenograft tumors(A) Expression levels for key nanocapsule entry & oligomer processing proteins were detected by immunoblot in PC3-LN4 & 22Rv1 cytosolic tumor lysates from the dose response studies. The signals for four mice per group are shown, the proteins detected are indicated on the right, & the size markers are indicated on the left. Two exposures are provided for GW182 in order to show detectable signals in linear range in all lanes for both PC3-LN4 & 22Rv1 tumor lysates. T1, T2, T3, & T4 labels indicate different tumors within the treatment & xenograft model groups. Antibody sourcing information is listed in Materials & Methods. Actin signal was used as the loading control. (B) Indirect immunofluorescence detection of GW182 proteins & GW bodies in PC3-LN4 tumors. Results from 3 mice treated with TNG-RNAi-CK2 & 3 mice treated with TBG-RNAi-F7 are shown. T1, T2, & T3 labels indicate different tumors. Antibody sourcing information is listed in Materials & Methods. Nuclei were counterstained with Sytox® Green. Scale bar represents 20 μm. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28751_rabbit-polyclonal-gw182-antibody-31020241615467.jpg)
![Western Blot: GW182 Antibody [NBP1-28751] - TNRC6A interacts with eIF4E2. (A) Plasmids expressing proteins indicated were transfected into HEK293T cells. At 48 h posttransfection, cells were lysed & the lysates were immunoprecipitated (IP) in the presence of RNase A. The precipitates were resolved on SDS-PAGE followed by Western blotting. (B) A plasmid expressing Flag-tagged eIF4E1 or eIF4E2 was transfected into HEK293T cells. At 48 h posttransfection, cells were lysed & the lysates were immunoprecipitated with the anti-TNRC6A antibody or control IgG in the presence of RNase A. The precipitates were resolved on SDS-PAGE followed by Western blotting. (C) Upper: schematic representation of TNRC6A truncation mutants. Lower: A plasmid expressing the TNRC6A mutant indicated & a plasmid expressing myc-tagged eIF4E2 were transiently transfected into HEK293T cells. At 48 h posttransfection, cells were lysed & the lysates were immunoprecipitated with anti-Flag antibody in the presence of RNase A followed by Western blotting. (D) Bacterially expressed Flag-tagged eIF4E2 or eIF4E1 was incubated with Glutathione Sepharose 4B bound fusion protein of GST & the C-terminal domain of TNRC6A (6A-C). The precipitates were washed & resolved on SDS-PAGE followed by commassie brilliant blue staining (lower) & Western blotting (upper) Image collected & cropped by CiteAb from the following publication (//academic.oup.com/proteincell/article/8/10/750/6765124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28751_rabbit-polyclonal-gw182-antibody-310202416163726.jpg)
![Western Blot: GW182 Antibody [NBP1-28751] - Downregulation of eIF4E2 increases the protein levels of endogenous IMP1. HeLa cells were transfected with siRNAs indicated. At 48 h posttransfection, cells were lysed. (A) A fraction of the lysate was subjected to SDS-PAGE followed by Western blotting. (B) The rest cell lysate was used to extract RNA, followed by RT-qPCR measurement of the RNA levels. Relative IMP1 protein levels were quantified with the Image J software & normalized with the beta -actin levels. Translational efficiency was calculated as relative protein level divided by mRNA level. Fold repression was calculated as the value in the presence of the control siRNA divided by that in the presence of the targeting siRNA. Data presented are means ± SD of three independent experiments. The P value is determined by two-tailed Student’s t test. ns, nonsignificant. *P < 0.05; **P < 0.01; ***P < 0.005. Ctrli, control siRNA; TNi, siRNAs targeting TNRC6A & TNRC6B; 4E2i, siRNA targeting eIF4E2; 4Ei, siRNA targeting eIF4E1 Image collected & cropped by CiteAb from the following publication (//academic.oup.com/proteincell/article/8/10/750/6765124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28751_rabbit-polyclonal-gw182-antibody-310202416165638.jpg)
![Western Blot: GW182 Antibody [NBP1-57134] - NMDAR-dependent increase in Ago2-GW182 interaction and Ago2 phosphorylation at S387 requires Akt activity. NMDAR-stimulated increase in Ago2-GW182 interaction is Akt-dependent. Cortical neuronal cultures were treated with Akti-1/2 (Akt inhibitor), chelerythrine (Chel, PKC inhibitor) or CT99021 (GSK-3beta inhibitor) 20 min before NMDA or vehicle application. Lysates were prepared 10 min after NMDA washout and immunoprecipitated with Ago2 antibodies or control IgG as shown. Proteins were detected by Western blotting. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embj.201797943) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP1-57134-img0003.jpg "Western Blot: GW182 Antibody [NBP1-57134] - NMDAR-dependent increase in Ago2-GW182 interaction and Ago2 phosphorylation at S387 requires Akt activity. NMDAR-stimulated increase in Ago2-GW182 interaction is Akt-dependent. Cortical neuronal cultures were treated with Akti-1/2 (Akt inhibitor), chelerythrine (Chel, PKC inhibitor) or CT99021 (GSK-3beta inhibitor) 20 min before NMDA or vehicle application. Lysates were prepared 10 min after NMDA washout and immunoprecipitated with Ago2 antibodies or control IgG as shown. Proteins were detected by Western blotting. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embj.201797943) licensed under a CC-BY license.")
![Western Blot: GW182 Antibody [NBP1-57134] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP1-57134-img0002.jpg)
![Western Blot: GW182 Antibody [NBP1-57134] - Endogenous Ago1‐GW182 & Ago1‐DDX6 interactions are unaffected by NMDAR stimulation (related to Fig 1)Cortical neuronal cultures were exposed to NMDA or vehicle for 3 min; lysates were prepared 10 min after NMDA washout & immunoprecipitated with Ago1 antibodies. Proteins were detected by Western blotting. Graph shows quantification of Ago1‐GW182 interaction, normalised to vehicle control; n = 5; t‐test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29712715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-57134_rabbit-polyclonal-gw182-antibody-310202415284534.jpg)
![Western Blot: GW182 Antibody [NBP1-57134] - Transient increase in GW182‐Ago2 interaction & S387 phosphorylation in response to NMDAR stimulationTransient increase in Ago2‐GW182 interaction. Cortical neuronal cultures were exposed to NMDA or vehicle for 3 min, & lysates were prepared 0, 3, 6, 10, 20 min after NMDA washout & immunoprecipitated with Ago2 antibodies or control IgG. Proteins were detected by Western blotting. The inputs are shown in (B). Graph shows quantification of Ago2‐GW182 interaction, normalised to vehicle control; n = 4. **P < 0.01, ***P < 0.001; one‐way ANOVA, Bonferroni post hoc test. Mean ± SEM.Transient increase in S387 phosphorylation & Akt activation. The same lysates from (A) (1% of input) were analysed by Western blotting using antibodies against pS387 Ago2, Ago2, pS473 Akt, Akt, GW182 & GAPDH as a loading control. Graphs show quantification of pS387 Ago2 levels normalised to total Ago2 (top) & pS473 Akt normalised to total Akt (bottom); n = 4. *P < 0.05; two‐way ANOVA, Bonferroni post hoc test. Mean ± SEM.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29712715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-57134_rabbit-polyclonal-gw182-antibody-31020241616085.jpg)
![Western Blot: GW182 Antibody [NBP1-57134] - NMDA‐induced increase in interaction with GW182 is caused by Ago2 phosphorylation at S387Ago2 S387A mutation blocks, & S387D mutation occludes NMDA‐induced increases in GW182 & DDX6 interactions. Cortical neurons were transfected with molecular replacement constructs expressing Ago2 shRNA plus shRNA‐resistant GFP‐Ago2 (WT, S387A or S387D). Lysates were prepared 10 min after NMDA washout, & GFP‐Ago2 complexes were precipitated using GFP‐trap beads. Bound proteins were detected by Western blotting using GFP, GW182, MOV10, DDX6 or PICK1 antibodies as shown. Graphs show quantification of GFP‐Ago2 interactions, normalised to untreated WT condition; n = 5. *P < 0.05, **P < 0.01; two‐way ANOVA, Bonferroni post hoc test. Mean ± SEM.Ago2 S387A mutation blocks, & S387D mutation occludes NMDA‐induced increase in GW182 co‐localisation in neuronal dendrites. Cortical neurons were transfected with molecular replacement constructs expressing Ago2 shRNA plus shRNA‐resistant GFP‐Ago2 (WT, S387A or S387D), fixed 10 min after NMDA washout, permeabilised & stained with GW182 & GFP antibodies. Graph shows Pearson's co‐localisation coefficients; n = 4 independent experiments (11 cells per condition). *P < 0.05, **P < 0.01; two‐way ANOVA, Bonferroni post hoc test. Scale bar = 10 μm. Mean ± SEM.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29712715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-57134_rabbit-polyclonal-gw182-antibody-31020241616053.jpg)
![Western Blot: GW182 Antibody [NBP1-57134] - Ago2 association with GW182 in neuronal dendrites increases in response to NMDAR stimulationEndogenous Ago2‐GW182 & Ago2‐DDX6 interactions increase in response to NMDAR stimulation. Cortical neuronal cultures were exposed to NMDA or vehicle for 3 min, & lysates were prepared 10 min after NMDA washout & immunoprecipitated with Ago2 antibodies. Proteins were detected by Western blotting. Graph shows quantification of Ago2‐GW182 interaction, normalised to vehicle control; n = 5. *P < 0.05; ***P < 0.001; t‐test; mean ± SEM.Analysis of endogenous Ago2‐GW182 co‐localisation in cortical neuronal cultures. Cortical neuronal cultures were exposed to NMDA or vehicle for 3 min, fixed 10 min after NMDA washout, permeabilised & co‐stained with Ago2 & GW182 antibodies. Representative whole‐cell images are shown. Scale bar = 50 μm.Endogenous GW182‐Ago2 co‐localisation increases in response to NMDAR stimulation in neuronal dendrites. Images show dendrites taken from boxed region in (B), above. Graph shows Pearson's co‐localisation coefficients; n = 4 independent experiments (18–24 cells per condition). *P < 0.05, t‐test. Scale bar = 10 μm. Mean ± SEM.Line‐scan analyses of Ago2 & GW182 fluorescence intensities in control & NMDA‐stimulated dendrites shown in (C).NMDAR stimulation has no effect on endogenous Ago2‐GW182 co‐localisation in neuronal cell bodies. Images show cell bodies taken from boxed region in (B). Graph shows Pearson's co‐localisation coefficients; n = 4 independent experiments (18–20 cells per condition), t‐test. Scale bar = 10 μm. Mean ± SEM.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29712715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-57134_rabbit-polyclonal-gw182-antibody-31020241616089.jpg)
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human prostate shows strong nuclear combined with weaker granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0005.jpg "Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human prostate shows strong nuclear combined with weaker granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human endometrium shows strong nuclear and moderate cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0003.jpg)
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0004.jpg)
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human skeletal muscle shows very weak nuclear and cytoplasmic positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0006.jpg)
![Western Blot: GW182 Antibody [NBP3-03014] - Analysis of extracts of various cell lines, using GW182 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP3-03014-img0002.jpg "Western Blot: GW182 Antibody [NBP3-03014] - Analysis of extracts of various cell lines, using GW182 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: GW182 Antibody [NBP3-03014] - Analysis of U2OS cells using GW182 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03014-img0001.jpg)
![Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunoprecipitation-NBP1-28702-img0003.jpg "Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunoprecipitation-NBP1-28702-img0002.jpg)
![Immunohistochemistry: GW182 Antibody [NBP3-06657] - Immunochemical staining of human TNRC6A in human breast carcinoma with NBP3-06657 (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-NBP3-06657-img0001.jpg "Immunohistochemistry: GW182 Antibody [NBP3-06657] - Immunochemical staining of human TNRC6A in human breast carcinoma with NBP3-06657 (1:300, formalin-fixed paraffin embedded sections).")