MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="K562 human chronic myelogenous leukemia cell line was stained with Human IL‑17B Monoclonal Antibody (Catalog # MAB1248, filled histogram) or isotype control antibody (Catalog #
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
1248-IB) was serially diluted and captured by Mouse Anti-Human IL‑17B Monoclonal Antibody (Catalog # MAB1248) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human IL‑17B Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox">
1248-IB) was serially diluted and captured by Mouse Anti-Human IL‑17B Monoclonal Antibody (Catalog # MAB1248) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human IL‑17B Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />
AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="M-NFS-60 mouse myelogenous leukemia lymphoblast cell line was stained with Goat Anti-Mouse IL-17B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1709, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="K562 human chronic myelogenous leukemia cell line was stained with Human IL‑17B Monoclonal Antibody (Catalog # MAB1248, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
1709-ML) was serially diluted and captured by Goat Anti-Mouse IL‑17B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1709) coated on a Clear Polystyrene Microplate (Catalog # 
1709-ML) was serially diluted and captured by Goat Anti-Mouse IL‑17B Antigen Affinity-purified Polyclonal Antibody (Catalog # 
1248-IB) was serially diluted and captured by Mouse Anti-Human IL‑17B Monoclonal Antibody (Catalog # MAB1248) coated on a Clear Polystyrene Microplate (Catalog # ![N/A IL-17B [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL17B_DY1248_ELISA_1613.jpg "N/A IL-17B [Biotin]")
![Immunohistochemistry-Paraffin: IL-17B Antibody (MM0373-3D62) [NBP2-11672] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11672. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize/IL-17B-Antibody-MM0373-3D62-Immunohistochemistry-Paraffin-NBP2-11672-img0001.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IL-17B Antibody (MM0373-3D62) [NBP2-11672] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11672. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.")
![Western Blot: IL17B Antibody [NBP1-83113] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/IL17B-Antibody-Western-Blot-NBP1-83113-img0004.jpg "Western Blot: IL17B Antibody [NBP1-83113] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: IL17B Antibody [NBP1-83113] - Staining of human spleen shows moderate nuclear positivity in cells in red pulp.](http://images.novusbio.com/fullsize/IL17B-Antibody-Immunohistochemistry-Paraffin-NBP1-83113-img0002.jpg)
![Western Blot: IL17B Overexpression Lysate (Adult Normal) [NBP2-07333] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL17B.](http://images.novusbio.com/fullsize/IL17B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07333-img0001.jpg "Western Blot: IL17B Overexpression Lysate (Adult Normal) [NBP2-07333] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL17B.")
![SDS-Page: Human IL-17B Protein [NBP2-35044]](http://images.novusbio.com/fullsize/Human-IL-17B-Protein-SDS-Page-NBP2-35044-img0001.jpg "SDS-Page: Human IL-17B Protein [NBP2-35044]")
