VEGFR3/Flt-4: Novus Biologicals

AF743

VEGFR3/Flt-4 Antibody [Unconjugated]

(3 Reviews)

100 μg

AF743

AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGFR3/Flt-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGFR3/Flt-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha -GFP, green) near kdr-l positive ( alpha -RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries and veins. NG2 (red) labels pericytes and smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, and Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), and VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single and double-labelled cells in 2-month old mouse meninges. VEGFR3 and LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 and LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta . Cells co-expressing VEGFR3 and LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) and LYVE1 (d′, white) internalize A beta 1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) and MRC1 (e′, white) internalize A beta 1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) and LYVE1 (f′, white) internalize A beta 1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta 1-40. Scale = 60 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), and confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); and 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), and MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology and confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), and VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) and images are merged in (k, p). Scale = 10 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endothelial changes after pericyte depletion. a–f Maximum intensity projection of confocal images from control and DTRiPC P6 retinas stained for IB4 (red) in combination with VEGF-A a, VEGFR2 b, VEGFR3 c, Tie2 d, Esm1 e, and Dll4 f (all in white), as indicated. Note local increase of VEGFR2, VEGFR3, and Esm1 (arrowheads in b, c, e) but not Tie2 or VEGF-A at the edge of the vessel plexus. Dll4 expression in DTRiPC sprouts is increased in some regions (arrowheads) but absent in others (arrows). Scale bar, 100 µm. g–j Quantitation of VEGF-A immunosignals area and intensity g, signal intensity for VEGFR2 h and VEGFR3 i and proportion of Esm1+ area with respect to vascular area j in the P6 control and DTRiPC angiogenic front. Error bars, s.e.m. p-values, Student’s t-test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cultured murine LECs express VDR.To check lymphatic origin of mouse LECs, we used three different well-established markers expressed by LECs. (a) Lymphatic origin of murine LECs was confirmed by IHC for Prox-1, VEGFR3 and Podoplanin (400x). Scale bar: 50 μm. (b) VDR expression of in vitro grown murine LECs was evaluated by western blot. Murine renal tubular epithelial cells (MTCs) served as positive control. (c) VDR expression of murine LECs was assessed by immunofluorescence (antibody D6) staining (200x). Scale bar: 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep44403), licensed under a CC-BY license. Not internally tested by R&D Systems.

Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). Note blunt appearance of the vessel front after VEGF-A injection but not for vehicle (PBS) control. Scale bar, 200 µm. b Quantitation of sprouts and filopodia at the front of the P6 vessel plexus after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. c PDGFR beta + (green) pericytes are unaffected by short-term VEGF-A administration, whereas VEGFR2 immunosignals (white) are increased in IB4+ (red) ECs (arrowheads). Images shown correspond to insets in a. Scale bar, 100 µm. d Quantitation of VEGFR2 immunosignals intensity in the peripheral plexus of P6 retinas after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. e Confocal images showing increased Esm1 immunostaining (white) in IB4+ (red) ECs in the peripheral plexus (arrowheads) after VEGF-A injection in P6 pups. Scale bar, 200 µm. f VEGF-A165 injection-mediated increase of Esm1 immunosignals (normalized to IB4+ EC area) in the peripheral capillary plexus but not at the edge of the angiogenic front in comparison to PBS-injected controls at P6. Error bars, s.e.m. p-values, Student’s t-test. NS, not statistically significant. g Short-term VEGF-A165 administration leads to clustering of Erg1+ (green) and IB4+ (red) ECs, as indicated, in thick sprout-like structures of P6 retinas. Panels in the center and on the right (scale bar, 20 µm) show higher magnification of the insets on the left (scale bar, 100 µm). Dashed lines in panels on the right outline IB4+ vessels. h Quantitation of EC density in the leading front vessel and emerging sprouts of the P6 angiogenic front after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test Image collected and cropped by CiteAb from the following publication (//pubme

<b>Biological Strategies Validation. </b>Kinase activity of EphB4 is required for lymphatic valve development.(a) Visualization of mesenteric lymphatic vessels and valves (arrows) by staining for Prox-1 and VEGFR3 2 days following treatment of P3 neonatal mice with NVP-BHG712, a selective EphB4 inhibitor. Blood vessels are highlighted by strong alpha -smooth muscle actin ( alpha SMA) staining. Scale bar, 200 μm. (b) Quantification of mesenteric lymphatic valves, ***P< 0.001 (two-tailed, unpaired student's t-test), n=3 per treatment group (error bars, s.d.). (c) NVP-BHG712 inhibits EphB4 phosphorylation in P2 neonatal mice. Lung tissue lysates were subjected to anti-EphB4 immunoprecipitation followed by anti-pY or anti-EphB4 immunoblotting. Ratios of pEphB4 to total EphB4 (pEphB4: EphB4) are graphed. *P<0.05 (two-tailed, unpaired student's t-test), n=4 per treatment group (error bars, s.d.). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25865237), licensed under a CC-BY license. Not internally tested by R&D Systems.

YAP and TAZ are required for the maintenance of LVs. The lymphatic vessels in the dorsal skin of E16.5 and E18.5 control and Lyve1-Cre;Yapf/f;Tazf/f embryos were analyzed by whole-mount immunohistochemistry. (A,B) LVs were observed in the collecting lymphatic vessels of E16.5 control and Lyve1-Cre;Yapf/f;Tazf/f embryos (arrows). (C,D) The migrating front of E16.5 control (C) and Lyve1-Cre;Yapf/f;Tazf/f (D) embryos appeared comparable. (E-G) At E18.5, the lymphatic vessels from the left and right sides have merged to form a network in control embryos (E). In contrast, huge gaps were observed in between the migrating fronts of E18.5 Lyve1-Cre;Yapf/f;Tazf/f embryos (F, magenta lines). The lymphatic vessels of mutant embryos were also dilated. The distance between the migrating fronts and the diameter of vessels are quantified in G. (H,I) LVs were observed in the collecting lymphatic vessels of E18.5 control embryos (H, yellow arrows). In contrast, the dilated lymphatic vessels of E18.5 Lyve1-Cre;Yapf/f;Tazf/f embryos lacked LVs (I). The various parameters of lymphatic vascular patterning were quantified and are plotted in G. n=4 embryos per each genotype. ****P<0.0001. Data are mean±s.e.m. Scale bars: 200 µm in A-D; 500 µm in E,F; 200 µm in H,I. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33060128), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of dermal lymphatic vessels in WT and Vegfr2Y949F/Y949F embryos and adult mice.(A) Dermal lymphatic vessels in E14.5 WT and Vegfr2Y949F/Y949F (Y949F) embryos shown by immunostaining of Neuropilin2 (Nrp2) in the back skin. Scale bar, 500 μm. (B) Quantification of dermal lymphatic sprouting tips/mm2 in the E14.5 embryos. WT, n = 13; Y949F, n = 10. NS, not significant, t test. (C) Lymphatic vessel density measured by NRP2 staining and normalized to tissue area/field of view in the E14.5 embryos. WT, n = 7; Y949F, n = 6. NS, not significant, t test. (D) Representative images of LYVE1 immunofluorescence showing ear dermal lymphatic vasculature in 8–10-wk-old mice. Scale bar, 1 mm. (E) Quantification of LYVE1+ lymphatic vessel width in 8–10-wk-old mouse ear dermis. n = 4 mice/genotype. NS, not significant, t test. (F) Quantification of LYVE1+ lymphatic vessel density in the 8–10-wk-old mouse ear dermis. WT, n = 4; Y949F, n = 3. P = 0.0087, t test. (G) Expressions of VEGFR2, VEGFR3, and Podoplanin in isolated LYVE1+ cells from lungs of WT and Vegfr2Y949F/Y949F mice at postnatal day 10 and in HUVECs, detected by immunoblotting. (H) Quantification of expression levels normalized to GAPDH in (G).Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38148112), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF743U-100UG
Alexa Fluor 405	AF743V-100UG
Alexa Fluor 488	FAB743G-100UG
Alexa Fluor 532	AF743X-100UG
Alexa Fluor 594	AF743T-100UG
Alexa Fluor 647	AF743R-100UG
Alexa Fluor 700	AF743N-100UG
Alexa Fluor 750	AF743S-100UG
Alexa Fluor Plus 405	AF743AFP405-100UG
Alexa Fluor Plus 488	AF743AFP488-100UG
Alexa Fluor Plus 555	AF743AFP555-100UG
Alexa Fluor Plus 594	AF743AFP594-100UG
Alexa Fluor Plus 647	AF743AFP647-100UG
Alexa Fluor Plus 680	AF743AFP680-100UG
Allophycocyanin	FAB743A
Biotin	BAF743
Phycoerythrin	FAB743P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB743G-100UG
Carrier Free	FAB743A
Carrier Free	FAB743P
Carrier Free	AF743-SP

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AF349

VEGFR3/Flt-4 Antibody [Unconjugated]

(28 Publications)

100 μg

AF349

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR3/Flt-4 was detected in immersion fixed paraffin-embedded sections of human cervical squamous metaplasia using Human VEGFR3/Flt-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF349) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="VEGFR3/Flt-4 was detected in immersion fixed paraffin-embedded sections of human cervical squamous metaplasia using Human VEGFR3/Flt-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF349) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>TGF-beta 1, -beta 2 and -beta 3 reduce lymphatic marker expression in LECs.Primary human LECs were treated with 10, 20 or 30 ng/ml TGF-beta 1, -beta 2 and -beta 3 for 72 hours (a) or 100 hours (b). Untreated cells served as a control. Lysates were prepared and analysed by Western blot using antibodies specific for Lyve-1, Prox-1, VEGFR-3 or vimentin. Vinculin served as loading control. The experiment was performed twice with equivalent results. For densitometry evaluation, protein bands were analysed using the software ImageJ. Bands for the Prox-1, Lyve-1, vimentin and VEGFR-3 proteins were normalized to the corresponding loading control and are displayed as the expression level relative to the untreated control samples. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0162221), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Direct interaction between PI3K p85 and VEGFR-3.A, LEC whole cell lysate was immunoprecipitated with anti-PI3K or isotype control IgG followed by Western blotting of phospho-tyrosine (p-Tyr), VEGFR-3 or PI3K. LECs were treated with vehicle control (serum-free media ‘0′), IgG control, VEGF-C (100 ng/ml, C) or VEGF-A (100 ng/ml, A) ligand for 15 minutes. Control untreated LEC whole cell lysate is indicate by ‘WCL’ and Jurkat cell lysate served as a positive control (Jurkat). B, Detection of VEGFR-3/phospho-PI3K complexes (red spots) in vitro in LECs grown in absence (B, upper left panel) or presence (B, upper middle panel) of VEGF-C (100 ng/ml) for 15 minutes; CD31 staining (green); DAPI (blue); isotype IgG control in PLA (B, upper right panel); quantification of VEGFR-3/phospho-PI3K and VEGFR-3/total-PI3K PLA signals using Olink Imaging Software (B, lower panel). Data shown as mean±s.e.m. **P<0.01 using t-test analysis. n = 3. Bar = 50 µm. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0039558), licensed under a CC-BY license. Not internally tested by R&D Systems.

VEGF-C induces LEC tube formation.A, Prostatic LEC tube length at 4.5 hours post VEGF-C treatment was quantified using ImageJ. VEGF-C significantly increased the number of tubes formed compared to vehicle control. Data expressed as mean±s.e.m., n = 3, ***P<0.001 using One-way ANOVA, Bonferroni post-analysis. B, Western blotting analysis of VEGFR-2 and VEGFR-3 expression in lung, neonatal dermis and prostate LECs. beta -tubulin was used as a loading control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22745786), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF349U-100UG
Alexa Fluor 405	AF349V-100UG
Alexa Fluor 488	AF349G-100UG
Alexa Fluor 532	AF349X-100UG
Alexa Fluor 594	AF349T-100UG
Alexa Fluor 647	AF349R-100UG
Alexa Fluor 700	AF349N-100UG
Alexa Fluor 750	AF349S-100UG
Alexa Fluor Plus 405	AF349AFP405-100UG
Alexa Fluor Plus 488	AF349AFP488-100UG
Alexa Fluor Plus 555	AF349AFP555-100UG
Alexa Fluor Plus 594	AF349AFP594-100UG
Alexa Fluor Plus 647	AF349AFP647-100UG
Alexa Fluor Plus 680	AF349AFP680-100UG
Biotin	BAF349

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF349-SP

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349-F4

Recombinant Human VEGFR3/Flt-4 Fc Chimera Protein, CF

(18 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-18651

VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free

(2 Publications)

0.1 mg

NBP1-18651

Flow Cytometry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal lymphatic endothelial cells (HDLEC).

Flow Cytometry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC). There is only a very weak expression of VEGFR-3/FLT4 detectable in the HUVECs but a strong one in the HDMVEC.

Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with double-vessel CAD showing immunohistochemical staining. VEGF-C (a) & VEGFR-3 (b) reactivity in a graft wall obtained from a 67-year-old patient who developed the early graft restenosis within 7 months after coronary artery bypass grafting (the same patient as mentioned in Fig. 6). VEGF-C is localized exclusively within individual smooth muscle cells of the tunica media (TM). Cells with positive VEGFR-3 expression are situated in endothelial cells of the tunica intima (TI). c Negative control. TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free [NBP1-18651] - Immunohistochemical staining of an internal thoracic artery & saphenous vein wall harvested from a patient with no evidence of luminal stenosis 12 months after CABG. Immunohistochemical staining of VEGF-C (a) & CAV2 (b) in an internal thoracic artery (ITA) graft & VEGFR-3 (c) in saphenous vein (SV) transplant harvested from a 65-year-old patient diagnosed with triple-vessel CAD. Note that lack of CAV2 expression within smooth muscle cells (SMC) in ITA wall is accompanied by VEGF-C positive expression in the same area. In SV tissue sample harvested from the same patient, no VEGRR-3 expression was found. d Negative control. TI tunica intima, TM tunica media, TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-18651AF350
Alexa Fluor 405	NBP1-18651AF405
Alexa Fluor 488	NBP1-18651AF488
Alexa Fluor 532	NBP1-18651AF532
Alexa Fluor 594	NBP1-18651AF594
Alexa Fluor 647	NBP1-18651AF647
Alexa Fluor 700	NBP1-18651AF700
Alexa Fluor 750	NBP1-18651AF750
Alexa Fluor Plus 405	NBP1-18651AFP405
Alexa Fluor Plus 488	NBP1-18651AFP488
Alexa Fluor Plus 555	NBP1-18651AFP555
Alexa Fluor Plus 594	NBP1-18651AFP594
Alexa Fluor Plus 647	NBP1-18651AFP647
Alexa Fluor Plus 680	NBP1-18651AFP680
Allophycocyanin	NBP1-18651APC
Biotin	NBP1-18652
CoraFluor 1	NBP1-18651CL1
DyLight 350	NBP1-18651UV
DyLight 405	NBP1-18651V
DyLight 488	NBP1-18651G
DyLight 550	NBP1-18651R
DyLight 594	NBP1-18651DL594
DyLight 650	NBP1-18651C
DyLight 680	NBP1-18651FR
DyLight 755	NBP1-18651IR
FITC	NBP1-18651F
HRP	NBP1-18651H
Janelia Fluor 525	NBP1-18651JF525
Janelia Fluor 549	NBP1-18651JF549
Janelia Fluor 646	NBP1-18651JF646
Janelia Fluor 669	NBP1-18651JF669
PE	NBP1-18651PE
PerCP	NBP1-18651PCP
mFluor Violet 450 SE	NBP1-18651MFV450
mFluor Violet 500 SE	NBP1-18651MFV500
mFluor Violet 610 SE	NBP1-18651MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP1-18651-0.02mg

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NBP1-43259

VEGFR3/Flt-4 Antibody (AFL4) - BSA Free

(3 Publications)

0.1 mg

NBP1-43259

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43259AF350
Alexa Fluor 405	NBP1-43259AF405
Alexa Fluor 488	NBP1-43259AF488
Alexa Fluor 532	NBP1-43259AF532
Alexa Fluor 594	NBP1-43259AF594
Alexa Fluor 647	NBP1-43259AF647
Alexa Fluor 700	NBP1-43259AF700
Alexa Fluor 750	NBP1-43259AF750
Alexa Fluor Plus 405	NBP1-43259AFP405
Alexa Fluor Plus 488	NBP1-43259AFP488
Alexa Fluor Plus 555	NBP1-43259AFP555
Alexa Fluor Plus 594	NBP1-43259AFP594
Alexa Fluor Plus 647	NBP1-43259AFP647
Alexa Fluor Plus 680	NBP1-43259AFP680
Allophycocyanin	NBP1-43259APC
Allophycocyanin/Cy7	NBP1-43259APCCY7
Biotin	NBP1-43259B
CoraFluor 1	NBP1-43259CL1
DyLight 350	NBP1-43259UV
DyLight 405	NBP1-43259V
DyLight 488	NBP1-43259G
DyLight 550	NBP1-43259R
DyLight 594	NBP1-43259DL594
DyLight 650	NBP1-43259C
DyLight 680	NBP1-43259FR
DyLight 755	NBP1-43259IR
FITC	NBP1-43259F
HRP	NBP1-43259H
Janelia Fluor 525	NBP1-43259JF525
Janelia Fluor 549	NBP1-43259JF549
Janelia Fluor 646	NBP1-43259JF646
Janelia Fluor 669	NBP1-43259JF669
PE	NBP1-43259PE
PE/Cy7	NBP1-43259PECY7
PerCP	NBP1-43259PCP
mFluor Violet 450 SE	NBP1-43259MFV450
mFluor Violet 500 SE	NBP1-43259MFV500
mFluor Violet 610 SE	NBP1-43259MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43259-0.5mg
BSA Free	NBP1-43259-0.05mg

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NB110-61018

VEGFR3/Flt-4 Antibody (RM0003-5F63) - Azide and BSA Free

(2 Publications)

0.1 mg

NB110-61018

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-61018AF350
Alexa Fluor 405	NB110-61018AF405
Alexa Fluor 488	NB110-61018AF488
Alexa Fluor 532	NB110-61018AF532
Alexa Fluor 594	NB110-61018AF594
Alexa Fluor 647	NB110-61018AF647
Alexa Fluor 700	NB110-61018AF700
Alexa Fluor 750	NB110-61018AF750
Alexa Fluor Plus 405	NB110-61018AFP405
Alexa Fluor Plus 488	NB110-61018AFP488
Alexa Fluor Plus 555	NB110-61018AFP555
Alexa Fluor Plus 594	NB110-61018AFP594
Alexa Fluor Plus 647	NB110-61018AFP647
Alexa Fluor Plus 680	NB110-61018AFP680
Allophycocyanin	NB110-61018APC
Biotin	NB110-61018B
CoraFluor 1	NB110-61018CL1
DyLight 350	NB110-61018UV
DyLight 405	NB110-61018V
DyLight 488	NB110-61018G
DyLight 550	NB110-61018R
DyLight 594	NB110-61018DL594
DyLight 650	NB110-61018C
DyLight 680	NB110-61018FR
DyLight 755	NB110-61018IR
FITC	NB110-61018F
HRP	NB110-61018H
Janelia Fluor 525	NB110-61018JF525
Janelia Fluor 549	NB110-61018JF549
Janelia Fluor 646	NB110-61018JF646
Janelia Fluor 669	NB110-61018JF669
PE	NB110-61018PE
PerCP	NB110-61018PCP
mFluor Violet 450 SE	NB110-61018MFV450
mFluor Violet 500 SE	NB110-61018MFV500
mFluor Violet 610 SE	NB110-61018MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB110-61018-0.025mg

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743-R3

Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF

(10 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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DYC2724-2

Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA, 2 Plate

(6 Publications)

1 Kit (for 2 Plates)

DYC2724-2

BAM1676) to detect phospho-VEGF R3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004). Human Phospho-VEGF R3 can be detected in this DuoSet IC ELISA by using approximately 10 to 15 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox">

Lysates prepared from human VEGF R3 transfected NS0 cells (NS0-hVEGF R3) were diluted in a series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-VEGF R3 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # <a class=

BAM1676) to detect phospho-VEGF R3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004). Human Phospho-VEGF R3 can be detected in this DuoSet IC ELISA by using approximately 10 to 15 times less lysate than is needed for a conventional IP-Western blot." alt="Lysates prepared from human VEGF R3 transfected NS0 cells (NS0-hVEGF R3) were diluted in a series and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-VEGF R3 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-VEGF R3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004). Human Phospho-VEGF R3 can be detected in this DuoSet IC ELISA by using approximately 10 to 15 times less lysate than is needed for a conventional IP-Western blot." class="big_thumb" />

349-F4), human VEGF R1 (Catalog #321-FL), human VEGF R2 (Catalog #357-KD) or human Tie-2 (Catalog #313-TI) were added to 20 μg NS0-hVEGF R3 cell lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human VEGF R3. " class="big_lightbox">

The indicated amounts of recombinant extracellular domains of human VEGF R3 (Catalog #<a class=

349-F4), human VEGF R1 (Catalog #321-FL), human VEGF R2 (Catalog #357-KD) or human Tie-2 (Catalog #313-TI) were added to 20 μg NS0-hVEGF R3 cell lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human VEGF R3. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC2724-5

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MAB11624

VEGFR3/Flt-4 Antibody (1091512) [Unconjugated]

100 μg

MAB11624

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." alt="VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and cytoplasm of liver endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR3/Flt-4 was detected in immersion fixed paraffin-embedded sections of human liver using Rat Anti-Human VEGFR3/Flt-4 Monoclonal Antibody (Catalog # MAB11624) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and cytoplasm of liver endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11624U-100UG
Alexa Fluor 405	FAB11624V-100UG
Alexa Fluor 488	FAB11624G-100UG
Alexa Fluor 532	FAB11624X-100UG
Alexa Fluor 594	FAB11624T-100UG
Alexa Fluor 647	FAB11624R-100UG
Alexa Fluor 700	FAB11624N-100UG
Alexa Fluor 750	FAB11624S-100UG
Alexa Fluor Plus 405	FAB11624AFP405-100UG
Alexa Fluor Plus 488	FAB11624AFP488-100UG
Alexa Fluor Plus 555	FAB11624AFP555-100UG
Alexa Fluor Plus 594	FAB11624AFP594-100UG
Alexa Fluor Plus 647	FAB11624AFP647-100UG
Alexa Fluor Plus 680	FAB11624AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11624-SP

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MAB349

VEGFR3/Flt-4 Antibody (54716) [Unconjugated]

(2 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB349U-100UG
Alexa Fluor 405	FAB349V-100UG
Alexa Fluor 488	FAB349G-100UG
Alexa Fluor 532	FAB349X-100UG
Alexa Fluor 594	FAB349T-100UG
Alexa Fluor 647	FAB349R-100UG
Alexa Fluor 700	FAB349N-100UG
Alexa Fluor 750	FAB349S-100UG
Alexa Fluor Plus 405	FAB349AFP405-100UG
Alexa Fluor Plus 488	FAB349AFP488-100UG
Alexa Fluor Plus 555	FAB349AFP555-100UG
Alexa Fluor Plus 594	FAB349AFP594-100UG
Alexa Fluor Plus 647	FAB349AFP647-100UG
Alexa Fluor Plus 680	FAB349AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB349-SP
Carrier Free	MAB349-500

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MAB743

VEGFR3/Flt-4 Antibody (102806) [Unconjugated]

(2 Publications)

500 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB743U-100UG
Alexa Fluor 405	FAB743V-100UG
Alexa Fluor 532	FAB743X-100UG
Alexa Fluor 594	FAB743T-100UG
Alexa Fluor 647	FAB743R-100UG
Alexa Fluor 700	FAB743N-100UG
Alexa Fluor 750	FAB743S-100UG
Alexa Fluor Plus 405	FAB743AFP405-100UG
Alexa Fluor Plus 488	FAB743AFP488-100UG
Alexa Fluor Plus 555	FAB743AFP555-100UG
Alexa Fluor Plus 594	FAB743AFP594-100UG
Alexa Fluor Plus 647	FAB743AFP647-100UG
Alexa Fluor Plus 680	FAB743AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB743-SP

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NBP2-78875

Mouse VEGFR3/Flt-4 ELISA Kit (Colorimetric)

1 Kit

NBP2-78875

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

62.50 - 4000 pg/mL

Sensitivity:

37.50 pg/mL

Sample Type:

Serum, plasma, and other biological fluids

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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VEGFR3/Flt-4 Antibody (RM0003-5F63) - Azide and BSA Free

Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF

Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA, 2 Plate

VEGFR3/Flt-4 Antibody (1091512) [Unconjugated]

VEGFR3/Flt-4 Antibody (54716) [Unconjugated]

VEGFR3/Flt-4 Antibody (102806) [Unconjugated]

Mouse VEGFR3/Flt-4 ELISA Kit (Colorimetric)