Recombinant Human VEGFR3/Flt-4 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human VEGFR3/Flt-4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGFR3/Flt‑4 Fc Chimera at 5 μg/mL (100 μL/well) binds Recombinant Human VEGF-D (Catalog # 622-VD) with an apparent Kd <15 nM.
Source
Mouse myeloma cell line, NS0-derived human VEGFR3/Flt-4 protein
Human Flt-4
(Tyr25-Ile776) &
(Ser473-Ile776)
Accession # P35916
IEGRDMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Tyr25 & Ser473
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FLT4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
112 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
145-150 kDa, 90-95 kDa and 75-80 kDa, reducing conditions
Publications
Read Publications using
349-F4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGFR3/Flt-4 Fc Chimera Protein, CF

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt‑1) and VEGFR3 (Flt‑4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt‑4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

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349-F4
Species: Hu
Applications: Binding Activity

Publications for VEGFR3/Flt-4 (349-F4)(15)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Porcine.

We have publications tested in 6 applications: Bioassay, ELISA (Standard), ELISA Developmet, Enzyme Assay, Neutralization, Surface Plasmon Resonance.


Filter By Application
Bioassay
(9)
ELISA (Standard)
(1)
ELISA Developmet
(1)
Enzyme Assay
(2)
Neutralization
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(10)
Mouse
(1)
N/A
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 349-F4 Applications Species
J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Redox Biol, 2019;22(0):101116. 2019 [PMID: 30769285] (Neutralization, Human) Neutralization Human
SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017;7(1):16439. 2017 [PMID: 29180757] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Marc G Achen Differential receptor binding and regulatory mechanisms for the lymphangiogenic growth factors VEGF-C and VEGF-D J. Biol. Chem., 2016;0(0):. 2016 [PMID: 27852824] (Bioassay) Bioassay
Terrance G Johns Axitinib Has Antiangiogenic and Antitumorigenic Activity in Myxoid Liposarcoma Sarcoma, 2016;2016(0):3484673. 2016 [PMID: 27822137] (Bioassay, Human) Bioassay Human
Kyu-Yeon Han, Jin-Hong Chang, Hyun Lee, Dimitri T Azar Proangiogenic Interactions of Vascular Endothelial MMP14 With VEGF Receptor 1 in VEGFA-Mediated Corneal Angiogenesis. Investigative Ophthalmology & Visual Science, 2016;0(0):1552-5783. 2016 [PMID: 27327585] (Enzyme Assay, Human) Enzyme Assay Human
Harris, Nicole C, Davydova, Natalia, Roufail, Sally, Paquet-Fifield, Sophie, Paavonen, Karri, Karnezis, Tara, Zhang, You-Fang, Sato, Teruhiko, Rothacker, Julie, Nice, Edouard, Stacker, Steven A, Achen, Marc G The propeptides of VEGF-D determine heparin binding, receptor heterodimerization, and effects on tumor biology. J Biol Chem, 2013;288(12):8176-86. 2013 [PMID: 23404505] (Enzyme Assay, Human) Enzyme Assay Human
Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, Inoue N, Pereira L Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model. Am J Pathol, 2012;181(5):1540-59. 2012 [PMID: 22959908] (Bioassay, Human) Bioassay Human
Chien MH, Ku CC, Johansson G, Chen MW, Hsiao M, Su JL, Inoue H, Hua KT, Wei LH, Kuo ML Vascular endothelial growth factor-C (VEGF-C) promotes angiogenesis by induction of COX-2 in leukemic cells via the VEGF-R3/JNK/AP-1 pathway. Carcinogenesis, 2009;30(12):2005-13. 2009 [PMID: 19825968] (Bioassay, Human) Bioassay Human
Mouawad R, Spano JP, Comperat E, Capron F, Khayat D Tumoural expression and circulating level of VEGFR-3 (Flt-4) in metastatic melanoma patients: correlation with clinical parameters and outcome. Eur. J. Cancer, 2009;45(8):1407-14. 2009 [PMID: 19157860] (ELISA Developmet, N/A) ELISA Developmet N/A
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat. Cell Biol., 2008;10(5):527-37. 2008 [PMID: 18425119] (Bioassay, Human) Bioassay Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol FLT4
Uniprot