Products Related to Gene-ID: 22861

HAF016). Specific bands were detected for NLRP1/NALP1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox"> HAF016). Specific bands were detected for NLRP1/NALP1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with human NLRP1/NALP1 or transfected with NALP3, THP-1 human acute monocytic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human NLRP1/NALP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NLRP1/NALP1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="NLRP1/NALP1 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Mouse Anti-Human NLRP1/NALP1 Monoclonal Antibody (Catalog # MAB6788) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />