FGFR1: Novus Biologicals

NB600-1287

FGFR1 Antibody (M19B2) - BSA Free

(1 Review)

(7 Publications)

0.1 ml

NB600-1287

Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.

Reactivity:

Hu, Mu, Rt, Ca, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1287AF350
Alexa Fluor 405	NB600-1287AF405
Alexa Fluor 488	NB600-1287AF488
Alexa Fluor 532	NB600-1287AF532
Alexa Fluor 594	NB600-1287AF594
Alexa Fluor 647	NB600-1287AF647
Alexa Fluor 700	NB600-1287AF700
Alexa Fluor 750	NB600-1287AF750
Alexa Fluor Plus 405	NB600-1287AFP405
Alexa Fluor Plus 488	NB600-1287AFP488
Alexa Fluor Plus 555	NB600-1287AFP555
Alexa Fluor Plus 594	NB600-1287AFP594
Alexa Fluor Plus 647	NB600-1287AFP647
Alexa Fluor Plus 680	NB600-1287AFP680
Allophycocyanin	NB600-1287APC
Biotin	NB600-1287B
CoraFluor 1	NB600-1287CL1
DyLight 350	NB600-1287UV
DyLight 405	NB600-1287V
DyLight 488	NB600-1287G
DyLight 550	NB600-1287R
DyLight 594	NB600-1287DL594
DyLight 650	NB600-1287C
DyLight 680	NB600-1287FR
DyLight 755	NB600-1287IR
FITC	NB600-1287F
HRP	NB600-1287H
Janelia Fluor 525	NB600-1287JF525
Janelia Fluor 549	NB600-1287JF549
Janelia Fluor 646	NB600-1287JF646
Janelia Fluor 669	NB600-1287JF669
PE	NB600-1287PE
PerCP	NB600-1287PCP
mFluor Violet 450 SE	NB600-1287MFV450
mFluor Violet 500 SE	NB600-1287MFV500
mFluor Violet 610 SE	NB600-1287MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-1287SS
Azide and BSA Free	NBP2-80735

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NB100-2080

FGFR1 Antibody (M17D10) - BSA Free

(2 Publications)

0.1 ml

NB100-2080

Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody.

Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 protein was observed in SCC cells whereas the nuclei of SCC cells as well as the tumor stroma was largely negative for this protein.

Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for this protein. The stratum basale / bottom layer of keratinocytes in the epidermis showed a membrane-cytoplasmic stainin for FGF R1.

Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein.

Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2080AF350
Alexa Fluor 405	NB100-2080AF405
Alexa Fluor 488	NB100-2080AF488
Alexa Fluor 532	NB100-2080AF532
Alexa Fluor 594	NB100-2080AF594
Alexa Fluor 647	NB100-2080AF647
Alexa Fluor 700	NB100-2080AF700
Alexa Fluor 750	NB100-2080AF750
Alexa Fluor Plus 405	NB100-2080AFP405
Alexa Fluor Plus 488	NB100-2080AFP488
Alexa Fluor Plus 555	NB100-2080AFP555
Alexa Fluor Plus 594	NB100-2080AFP594
Alexa Fluor Plus 647	NB100-2080AFP647
Alexa Fluor Plus 680	NB100-2080AFP680
Allophycocyanin	NB100-2080APC
Biotin	NB100-2080B
CoraFluor 1	NB100-2080CL1
DyLight 350	NB100-2080UV
DyLight 405	NB100-2080V
DyLight 488	NB100-2080G
DyLight 550	NB100-2080R
DyLight 594	NB100-2080DL594
DyLight 650	NB100-2080C
DyLight 680	NB100-2080FR
DyLight 755	NB100-2080IR
FITC	NB100-2080F
HRP	NB100-2080H
Janelia Fluor 525	NB100-2080JF525
Janelia Fluor 549	NB100-2080JF549
Janelia Fluor 646	NB100-2080JF646
Janelia Fluor 669	NB100-2080JF669
PE	NB100-2080PE
PerCP	NB100-2080PCP
mFluor Violet 450 SE	NB100-2080MFV450
mFluor Violet 500 SE	NB100-2080MFV500
mFluor Violet 610 SE	NB100-2080MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2080SS
Azide and BSA Free	NBP2-80733

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NB120-829

FGFR1 Antibody (M2F12) - BSA Free

(3 Publications)

0.1 ml

NB120-829

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in MCF-7 cells (1 x 10^6 cells/ml) stained with FGF r1 antibody (NB120-829, red peaks) at a 1:1000 dilution. Detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in HEK293 cells (2 x10^6) stained with FGF R1 antibody (NB120-829, red) at a 1:1000 dilution and detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-829AF350
Alexa Fluor 405	NB120-829AF405
Alexa Fluor 488	NB120-829AF488
Alexa Fluor 532	NB120-829AF532
Alexa Fluor 594	NB120-829AF594
Alexa Fluor 647	NB120-829AF647
Alexa Fluor 700	NB120-829AF700
Alexa Fluor 750	NB120-829AF750
Alexa Fluor Plus 405	NB120-829AFP405
Alexa Fluor Plus 488	NB120-829AFP488
Alexa Fluor Plus 555	NB120-829AFP555
Alexa Fluor Plus 594	NB120-829AFP594
Alexa Fluor Plus 647	NB120-829AFP647
Alexa Fluor Plus 680	NB120-829AFP680
Allophycocyanin	NB120-829APC
Biotin	NB120-829B
CoraFluor 1	NB120-829CL1
DyLight 350	NB120-829UV
DyLight 405	NB120-829V
DyLight 488	NB120-829G
DyLight 550	NB120-829R
DyLight 594	NB120-829DL594
DyLight 650	NB120-829C
DyLight 680	NB120-829FR
DyLight 755	NB120-829IR
FITC	NB120-829F
HRP	NB120-829H
Janelia Fluor 525	NB120-829JF525
Janelia Fluor 549	NB120-829JF549
Janelia Fluor 646	NB120-829JF646
Janelia Fluor 669	NB120-829JF669
PE	NB120-829PE
PerCP	NB120-829PCP
mFluor Violet 450 SE	NB120-829MFV450
mFluor Violet 500 SE	NB120-829MFV500
mFluor Violet 610 SE	NB120-829MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB120-829SS
Azide and BSA Free	NBP2-80734

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AF3285

FGFR1-4 [p Tyr653, p Tyr654, p Tyr654] Antibody [Unconjugated]

(11 Publications)

50 μg

AF3285

232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # <a class=

232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>FGF R1-4 phosphorylated at Y653/Y654 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

GNA inhibits cell proliferation in both NSCLC and erlotinib-resistant NSCLC by targeting FGFR and the downstream signaling pathways.a Cell viability was assessed using the CellTiter-Glo assay. After a 72 h treatment by GNA, CellTiter-Glo reagent was added directly to each well for a 10-min incubation. The plate was then read on a FlexStation 3 microplate luminometer. A dose−response curve was plotted, and the IC50 was calculated using GraphPad Prism software. Cell viability was assessed using MTT assay. After a 72 h treatment by GNA, 10 μl of 5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution was added to each well and incubated for 4 h at 37 °C, then the culture medium was removed and 100 μl of DMSO was added to each well. The absorbance was measured at 570 nm on a microplate reader. A dose−response curve was plotted, and the IC50 was calculated using GraphPad Prism software. b Caspase-3/7 activity induction was evaluated after 6 h of treatment with GNA at 10, 3.33 and 1.11 μM using the Caspase-Glo 3/7 kit from Promega in the HCC827, H1650, and HCC827ER cells, respectively. The bars in the graphs represent the mean fold induction relative to the DMSO control. c H1650 cells, HCC827 cells, and HCC827ER cells were treated with GNA at various dosages for 4 h and were then probed with specified antibodies. FGFR and the downstream proteins were analyzed by western blot. d The histograms of quantitative analysis about FGFR and the downstream proteins were analyzed by western blot Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29449529), licensed under a CC-BY license. Not internally tested by R&D Systems.

GNA enhances the anti-tumor activity of erlotinib through FGFR in vitro.a H1650 cells, HCC827 cells, and HCC827ER cells were treated with various concentrations of GNA, erlotinib, or their combination for 24 h and were then probed with specified antibodies. b−e FGFR and the downstream proteins were analyzed by a western blot. HCC827ER cells were transfected with FGFR1 and FGFR2 siRNA for 48 h, then the medium was replaced and the cells were further incubated for 24 h. After incubation, the protein expression of FGFR1 (b) and FGFR2 (c) were analyzed by western blot, the mRNA expression of FGFR1 (d) and FGFR2 (e) were analyzed by real-time PCR. (f, g) FGFR1 or FGFR2 knockdown HCC827ER cells were treated by GNA for 72 h, and the cell viability was assessed using the CellTiter-Glo assay. The data are presented as the means ± SD and were analyzed by a one-way ANOVA, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29449529), licensed under a CC-BY license. Not internally tested by R&D Systems.

GNA increases the anti-tumor activity of erlotinib in FGFR-expressing PDX xenograft model. A The fragments from the donor mice that were implanted from patient samples were implanted subcutaneously into the right flank of nude mice. When the tumors reached 150–250 mm3, the tumor-bearing mice were then treated with vehicle, GNA (i.p., 7.5 mg/kg), erlotinib (p.o., 25 mg/kg), or their combination once daily for 22 days. The tumor volume was calculated by determining the length and width of the tumor as measured using Vernier calipers. The data are presented as the means ± SEM (n = 6) and were analyzed by a one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001. b The body weight of the mice from (a). c The tumor/body weight ratio of the mice from (a). d−f Representative immunohistochemical image analyses for p-FGFR (d, e) and apoptotic cells by a TUNEL assay (d, f) in the xenograft mouse model of the PDX tumors after vehicle, GNA, erlotinib, and combination treatment. The data are presented as the Histoscore and the error bars are the SEM. Statistically significant differences with P < 0.05 were considered significant compared with the vehicle control, *P < 0.05, and **P < 0.01. g Inhibition of FGFR phosphorylation in NSCLC PDX by GNA. After treatment with GNA, erlotinib, and its combination for about 3 weeks, the tumors were collected and processed. The protein samples were then probed with specified antibodies Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29449529), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3285-SP

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MAB765

FGFR1 Antibody (133111) - (IIIb)

(8 Publications)

100 μg

MAB765

655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL)." class="big_lightbox">

Recombinant Human FGF R1a (IIIb) Fc Chimera (Catalog # <a class=

655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL)." alt="Recombinant Human FGF R1a (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL)." class="big_thumb" />

<b>Biological Strategies Validation. </b>Proximity between AcSDKP and FGFR1 inhibits the TGF beta /smad signaling pathway in HMVECs. (a) HMVECs were treated with N-FGFR1 (1.5 μg/ml) for 48 h with or without preincubation with AcSDKP (100 nM) for 2 h, and the proximity between AcSDKP and FGFR1 was analyzed by the Duolink In Situ Assay. For each slide, images at a × 400 original magnification were obtained from six different areas. (b and c) HMVECs were treated with TGF beta 2 (5 ng/ml) for 15 min or 48 h with or without preincubation with AcSDKP for 2 h, and the p-smad3, TGF beta R1, TGF beta R2 and FGFR1 levels were analyzed by western blot. Densitometric analysis of the p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin levels from each group (n=6) were analyzed. (d and e) HMVECs were incubated with TGF beta 2 for 15 min or 48 h with or without preincubation with AcSDKP or its mutants (AcDSPK, AcSDKA, AcADKP) (100 nM) for 2 h. The p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin protein levels were analyzed by western blot Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28771231), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of FGFR1 in cells from breast and bone tissue. a, FGFR mRNA transcript levels in normal and cancer cells. b, Fold change in FGFR mRNA based on expression in cancers cells relative to normal cells. c, FGFR protein levels in normal versus cancer cells. Results are from triplicate experiments and the Western blot is representative of the triplicates. (*p < 0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26201468), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB765U-100UG
Alexa Fluor 405	FAB765V-100UG
Alexa Fluor 488	FAB765G-100UG
Alexa Fluor 532	FAB765X-100UG
Alexa Fluor 594	FAB765T-100UG
Alexa Fluor 647	FAB765R-100UG
Alexa Fluor 700	FAB765N-100UG
Alexa Fluor 750	FAB765S-100UG
Alexa Fluor Plus 405	FAB765AFP405-100UG
Alexa Fluor Plus 488	FAB765AFP488-100UG
Alexa Fluor Plus 555	FAB765AFP555-100UG
Alexa Fluor Plus 594	FAB765AFP594-100UG
Alexa Fluor Plus 647	FAB765AFP647-100UG
Alexa Fluor Plus 680	FAB765AFP680-100UG
Allophycocyanin	FAB765A
Biotin	FAB765B
CoraFluor 1	FAB765CL1
DyLight 350	FAB765D
DyLight 405	FAB765E
DyLight 488	FAB765K
DyLight 550	FAB765L
DyLight 594	FAB765M
DyLight 650	FAB765W
DyLight 680	FAB765Y
DyLight 755	FAB765Z
FITC	FAB765F
HRP	FAB765H
Janelia Fluor 525	FAB765JF525
Janelia Fluor 549	FAB765I
Janelia Fluor 646	FAB765J
Janelia Fluor 669	FAB765JF669
PE	FAB765P
PerCP	FAB765C
mFluor Violet 450 SE	FAB765MFV450
mFluor Violet 500 SE	FAB765MFV500
mFluor Violet 610 SE	FAB765MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB765-SP
Carrier Free	MAB765-500

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NB100-2079

FGFR1 Antibody (M17A3) - BSA Free

(2 Publications)

0.1 ml

NB100-2079

Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2079AF350
Alexa Fluor 405	NB100-2079AF405
Alexa Fluor 488	NB100-2079AF488
Alexa Fluor 532	NB100-2079AF532
Alexa Fluor 594	NB100-2079AF594
Alexa Fluor 647	NB100-2079AF647
Alexa Fluor 700	NB100-2079AF700
Alexa Fluor 750	NB100-2079AF750
Alexa Fluor Plus 405	NB100-2079AFP405
Alexa Fluor Plus 488	NB100-2079AFP488
Alexa Fluor Plus 555	NB100-2079AFP555
Alexa Fluor Plus 594	NB100-2079AFP594
Alexa Fluor Plus 647	NB100-2079AFP647
Alexa Fluor Plus 680	NB100-2079AFP680
Allophycocyanin	NB100-2079APC
Biotin	NB100-2079B
CoraFluor 1	NB100-2079CL1
DyLight 350	NB100-2079UV
DyLight 405	NB100-2079V
DyLight 488	NB100-2079G
DyLight 550	NB100-2079R
DyLight 594	NB100-2079DL594
DyLight 650	NB100-2079C
DyLight 680	NB100-2079FR
DyLight 755	NB100-2079IR
FITC	NB100-2079F
HRP	NB100-2079H
Janelia Fluor 525	NB100-2079JF525
Janelia Fluor 549	NB100-2079JF549
Janelia Fluor 646	NB100-2079JF646
Janelia Fluor 669	NB100-2079JF669
PE	NB100-2079PE
PerCP	NB100-2079PCP
mFluor Violet 450 SE	NB100-2079MFV450
mFluor Violet 500 SE	NB100-2079MFV500
mFluor Violet 610 SE	NB100-2079MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2079SS
Azide and BSA Free	NBP2-80732

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661-FR

Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF

(9 Publications)

50 μg

661-FR

1 μg/lane of Recombinant Human FGF R1 beta (IIIc) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 90 kDa and 180 kDa, respectively.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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658-FR

Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

(6 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-24462

FGFR1 [p Tyr651, p Tyr652] Antibody

(2 Publications)

0.02 mg

NBP2-24462

Reactivity:

Hu, Mu, Rt, Ch, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ze

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6448A

Alt Catalog# IMG-6448A

Conjugate:

Unconjugated

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MAB658

FGFR1 Antibody (133105) [Unconjugated]

(3 Publications)

100 μg

MAB658

Western blot confirmation of marker proteins. Equal quantities of protein from media supernatant from cells cultured as described in Fig 1 were run on Western blot and probed with antibodies for an E8-enriched marker protein (CHGA, NID1, SEMA3 or NPTX3) and an E6-enriched marker protein (COCH, FGFR1, Follistatin or OLFML3). Membranes were imaged using LICOR Odyssey system. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38875182), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB658U-100UG
Alexa Fluor 405	FAB658V-100UG
Alexa Fluor 488	FAB658G-100UG
Alexa Fluor 532	FAB658X-100UG
Alexa Fluor 594	FAB658T-100UG
Alexa Fluor 647	FAB658R-100UG
Alexa Fluor 700	FAB658N-100UG
Alexa Fluor 750	FAB658S-100UG
Alexa Fluor Plus 405	FAB658AFP405-100UG
Alexa Fluor Plus 488	FAB658AFP488-100UG
Alexa Fluor Plus 555	FAB658AFP555-100UG
Alexa Fluor Plus 594	FAB658AFP594-100UG
Alexa Fluor Plus 647	FAB658AFP647-100UG
Alexa Fluor Plus 680	FAB658AFP680-100UG
Allophycocyanin	FAB658A
Biotin	FAB658B
CoraFluor 1	FAB658CL1
DyLight 350	FAB658D
DyLight 405	FAB658E
DyLight 488	FAB658K
DyLight 550	FAB658L
DyLight 594	FAB658M
DyLight 650	FAB658W
DyLight 680	FAB658Y
DyLight 755	FAB658Z
FITC	FAB658F
HRP	FAB658H
Janelia Fluor 525	FAB658JF525
Janelia Fluor 549	FAB658I
Janelia Fluor 646	FAB658J
Janelia Fluor 669	FAB658JF669
PE	FAB658P
PerCP	FAB658C
mFluor Violet 450 SE	FAB658MFV450
mFluor Violet 500 SE	FAB658MFV500
mFluor Violet 610 SE	FAB658MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB658-SP
Carrier Free	MAB658-500

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765-FR

Recombinant Human FGFR1 beta (IIIb) Fc Chimera Protein, CF

(4 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DYC5079-2

Human Phospho-FGFR1 DuoSet IC ELISA, 2 Plate

(4 Publications)

1 Kit (for 2 Plates)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC5079E
Biotin	DYC5079-5

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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