| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB91871-SP | |||
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="MLKL phosphorylated at T357 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Phospho-MLKL (T357) Monoclonal Antibody (Catalog # MAB9187) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9187-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21586-50ul | |||
![Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using MLKL [p Ser345] Antibody (JM92-37). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLKL-p-Ser345-Antibody-JM92-37-Immunohistochemistry-Paraffin-NBP2-66953-img0004.jpg "<b>Biological Strategies Validation. </b>Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using MLKL [p Ser345] Antibody (JM92-37). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![<b>Biological Strategies Validation. </b>Immunohistochemical analysis of paraffin-embedded mouse colon tissue using MLKL [p Ser345] Antibody (JM92-37). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLKL-p-Ser345-Antibody-JM92-37-Immunohistochemistry-Paraffin-NBP2-66953-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemical analysis of paraffin-embedded mouse liver tissue using MLKL [p Ser345] Antibody (JM92-37). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLKL-p-Ser345-Antibody-JM92-37-Immunohistochemistry-Paraffin-NBP2-66953-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemical analysis of paraffin-embedded mouse lung tissue using MLKL [p Ser345] Antibody (JM92-37). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLKL-p-Ser345-Antibody-JM92-37-Immunohistochemistry-Paraffin-NBP2-66953-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western blot analysis of MLKL [p Ser345] on different lysates with MLKL [p Ser345] Antibody (JM92-37) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.Lane 1: L-929 cell lysateLane 2: L929 treated with 20μM Z-VAD for 3.5 hours, then added 100nM SM-164 and 20ng/ml TNF-alpha for 3 hours cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute 10 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66953_rabbit-monoclonal-mlkl-p-ser345-antibody-jm92-37-western-blot-2412024103144..jpg)
![<b>Biological Strategies Validation. </b>IHC-Frozen:MLKL [p Ser345] Antibody (JM92-37)-Analysis of frozen mouse spleen tissue with Rabbit anti-Phospho-MLKL (S345) antibody at 1/100 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-66953_rabbit-monoclonal-mlkl-p-ser345-antibody-jm92-37-immunocytochemistry-immunofluorescence-3072024102815..jpg)
![Western blot analysis on different lysates with NBP2-66953 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: L-929 cell lysate Lane 2: L929 treated with 20μM Z-VAD for 3.5 hours, then added 100nM SM-164 and 20ng/ml TNF-alpha for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66953 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66953_-western-blot-639204586669305604.jpg)
![Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in thymocytes.](http://images.novusbio.com/fullsize/MLKL-p-Thr357-Antibody-954724-Immunohistochemistry-Paraffin-MAB91871-img0002.jpg "Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in thymocytes.")
![Immunohistochemistry: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/MLKL-p-Thr357-Antibody-954724-Immunohistochemistry-MAB91871-img0001.jpg)
![Western Blot: MLKL Antibody [NBP1-56729] - Human fetal kidney lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/MLKL-Antibody-Western-Blot-NBP1-56729-img0002.jpg "Western Blot: MLKL Antibody [NBP1-56729] - Human fetal kidney lysate, concentration 0.2-1 ug/ml.")
![Western Blot: MLKL Antibody [NBP1-56729] - 293T Whole Cell lysates, Antibody Dilution: 1 ug/ml.](http://images.novusbio.com/fullsize/MLKL-Antibody-Western-Blot-NBP1-56729-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MLKL-Antibody-3B2-Immunocytochemistry-Immunofluorescence-H00197259-M02-img0005.jpg "Immunocytochemistry/Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MLKL-Antibody-3B2-Immunohistochemistry-Paraffin-H00197259-M02-img0006.jpg)
![ELISA: MLKL Antibody (3B2) [H00197259-M02] - Detection limit for recombinant GST tagged MLKL is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MLKL-Antibody-3B2-ELISA-H00197259-M02-img0008.jpg)
![Western Blot: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: HUVEC Lane 3: Hela](http://images.novusbio.com/fullsize/MLKL-Antibody-SA40-04-Western-Blot-NBP2-67299-img0002.jpg "Western Blot: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: HUVEC Lane 3: Hela")
![Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of paraffin-embedded human tonsil tissue using anti-MLKL antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MLKL-Antibody-SA40-04-Immunohistochemistry-Paraffin-NBP2-67299-img0001.jpg)
![Western blot analysis on different lysates with NBP2-67299 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: U-937 cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (low expression) (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67299 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67299_-western-blot-639204586671437426.jpg)
![Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL polyclonal antibody. Lane 1: MLKL transfected lysate(51.81 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MLKL-Antibody-Western-Blot-H00197259-B01P-img0004.jpg "Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL polyclonal antibody. Lane 1: MLKL transfected lysate(51.81 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MLKL Antibody [H00197259-B01P] - Analysis of purified antibody to MLKL on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MLKL-Antibody-Immunocytochemistry-Immunofluorescence-H00197259-B01P-img0003.jpg)
![Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL MaxPab polyclonal antibody. Lane 1: MLKL transfected lysate (51.81 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MLKL-Antibody-Western-Blot-H00197259-B01P-img0001.jpg)
![Western Blot: MLKL [p Ser 358] Antibody (SR1963) [NBP3-21586] - Western blot analysis using NBP3-21586 in (1) HT-29 cell lysate; (2) HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VAD.](http://images.novusbio.com/fullsize/nbp3-21586_rabbit-mlkl-p-ser-358-mab-sr1963-57202316582172.jpg "<b>Biological Strategies Validation. </b>Western Blot: MLKL [p Ser 358] Antibody (SR1963) [NBP3-21586] - Western blot analysis using NBP3-21586 in (1) HT-29 cell lysate; (2) HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VAD.")
![Western Blot: MLKL Antibody (7L1P5) [NBP3-15859] - Analysis of extracts of various cell lines, using MLKL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15859_rabbit-mlkl-mab-7l1p5-western-blot-137202391524..jpg "Western Blot: MLKL Antibody (7L1P5) [NBP3-15859] - Analysis of extracts of various cell lines, using MLKL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
