DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog #
BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog #
BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Mouse alpha ‑Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog #
BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse alpha ‑Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![N/A alpha-Fetoprotein/AFP [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_alphaFetoprotein_DY1369_ELISA_1635.jpg "N/A alpha-Fetoprotein/AFP [Biotin]")
![N/A alpha-Fetoprotein/AFP [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_alphaFetoprotein_DY536905_ELISA_2478.jpg "N/A alpha-Fetoprotein/AFP [Biotin]")
DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # ![Western Blot: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). NBP2-82221 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Western-Blot-NBP2-82221-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). NBP2-82221 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-82221, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Immunocytochemistry-Immunofluorescence-NBP2-82221-img0001.jpg)
![Flow Cytometry: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of ([NBP2-82221], blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Flow-Cytometry-NBP2-82221-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (MBS-12) [NBP2-45013] - Human Fetal Liver stained with AFP Monoclonal Antibody (MBS-12).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-MBS-12-Immunohistochemistry-Paraffin-NBP2-45013-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (MBS-12) [NBP2-45013] - Human Fetal Liver stained with AFP Monoclonal Antibody (MBS-12).")
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) [NBP2-45011] - Immunofluorescence Analysis of PFA-fixed HepG2 cells labeling AFP using alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C2-+-C3-+-MBS-12-Immunocytochemistry-Immunofluorescence-NBP2-45011-img0002.jpg "Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) [NBP2-45011] - Immunofluorescence Analysis of PFA-fixed HepG2 cells labeling AFP using alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) [NBP2-45011] - Human Fetal Liver stained with AFP Monoclonal Antibody (C2 + C3 + MBS-12).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C2-+-C3-+-MBS-12-Immunohistochemistry-Paraffin-NBP2-45011-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C2) [NBP2-45014] - Human Fetal Liver stained with AFP Monoclonal Antibody (C2).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C2-Immunohistochemistry-Paraffin-NBP2-45014-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C2) [NBP2-45014] - Human Fetal Liver stained with AFP Monoclonal Antibody (C2).")
![Western Blot: alpha-Fetoprotein/AFP Antibody (SR2120) [NBP3-21716] - Western blot analysis using NBP3-21716 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21716_rabbit-alpha-fetoprotein-afp-mab-sr2120-672023106282.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (SR2120) [NBP3-21716] - Western blot analysis using NBP3-21716 in HepG2 cell lysate.")
![Western Blot: alpha-Fetoprotein/AFP Antibody [NBP2-80483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-Western-Blot-NBP2-80483-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody [NBP2-80483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue")
![Immunohistochemistry: alpha-Fetoprotein/AFP Antibody [NBP2-80483] - Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-Immunohistochemistry-NBP2-80483-img0001.jpg)
