| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-82221SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90069-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48015-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14053 | |||
![Western Blot: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). NBP2-82221 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Western-Blot-NBP2-82221-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). NBP2-82221 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-82221, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Immunocytochemistry-Immunofluorescence-NBP2-82221-img0001.jpg)
![Flow Cytometry: alpha-Fetoprotein/AFP Antibody (AF5) [NBP2-82221] - HepG2 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of ([NBP2-82221], blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AF5-Flow-Cytometry-NBP2-82221-img0003.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody [NBP2-80483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-Western-Blot-NBP2-80483-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody [NBP2-80483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue")
![Immunohistochemistry: alpha-Fetoprotein/AFP Antibody [NBP2-80483] - Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-Immunohistochemistry-NBP2-80483-img0001.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-003-Western-Blot-NBP2-90069-img0001.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa")
![Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (003) [NBP2-90069] - Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-AFP rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-AFP rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 68 kDaObserved band size: 68 kDa](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-003-Immunoprecipitation-NBP2-90069-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (MBS-12) - IHC-Prediluted [NBP2-48120] - Human Fetal Liver stained with AFP Mouse Monoclonal Antibody (MBS-12).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-MBS-12-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48120-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (MBS-12) - IHC-Prediluted [NBP2-48120] - Human Fetal Liver stained with AFP Mouse Monoclonal Antibody (MBS-12).")
![Western Blot: alpha-Fetoprotein/AFP Overexpression Lysate [NBL1-07375] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha 1 Fetoprotein.](http://images.novusbio.com/fullsize/Alpha-1-Fetoprotein-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07375-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Overexpression Lysate [NBL1-07375] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha 1 Fetoprotein.")
![Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (PD00-71) [NBP3-32008] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-alpha-Fetoprotein/AFP antibody (NBP3-32008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32008_rabbit-alpha-fetoprotein-afp-mab-pd00-71-298202414304118.jpg "Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (PD00-71) [NBP3-32008] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-alpha-Fetoprotein/AFP antibody (NBP3-32008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (AFP/7007R) - Azide and BSA Free [NBP3-14053] - IHC analysis of formalin-fixed, paraffin-embedded human placenta. Cytoplasmic staining using alpha-Fetoprotein/AFP antibody (AFP/7007R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-14053_rabbit-alpha-fetoprotein-afp-mab-afp-7007r-azide-bsa-free-24120258211635.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (AFP/7007R) - Azide and BSA Free [NBP3-14053] - IHC analysis of formalin-fixed, paraffin-embedded human placenta. Cytoplasmic staining using alpha-Fetoprotein/AFP antibody (AFP/7007R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.")
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (AFP/7007R) [NBP3-13870] - IHC analysis of formalin-fixed, paraffin-embedded human placenta. Cytoplasmic staining using alpha-Fetoprotein/AFP antibody (AFP/7007R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-AFP-7007R-Immunohistochemistry-Paraffin-NBP3-13870-img0001.jpg "Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (AFP/7007R) [NBP3-13870] - IHC analysis of formalin-fixed, paraffin-embedded human placenta. Cytoplasmic staining using alpha-Fetoprotein/AFP antibody (AFP/7007R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.")