HAF018). A specific band was detected for Casein Kinase 2a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Casein Kinase 2a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7957-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57124-25ul | |||
NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for 
CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons. View our protocol for 
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 or 0.1 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0005.jpg)
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0012.jpg)
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg "Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western blot analysis on different lysates with NBP3-32228 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: Lane 1-7 (left): 40 seconds; Lane 1-7 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32228 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_-western-blot-639204597488042700.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody (M01), clone 3D9.Lane 1: CSNK2A1 transfected lysate(45.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Western-Blot-H00001457-M01-img0014.jpg "Western Blot: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody (M01), clone 3D9.Lane 1: CSNK2A1 transfected lysate(45.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of monoclonal antibody to CSNK2A1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Immunocytochemistry-Immunofluorescence-H00001457-M01-img0011.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - CSNK2A1 monoclonal antibody (M01), clone 3D9 Analysis of CSNK2A1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Western-Blot-H00001457-M01-img0013.jpg)
![Proximity Ligation Assay: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Analysis of protein-protein interactions between TP53 and CSNK2A1. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CSNK2A1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Proximity-Ligation-Assay-H00001457-M01-img0009.jpg)
![Sandwich ELISA: Casein Kinase 2 alpha Antibody (3D9) [H00001457-M01] - Detection limit for recombinant GST tagged CSNK2A1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-3D9-Sandwich-ELISA-H00001457-M01-img0012.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-378-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-NB100-378-img0010.jpg)
![Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 (lot NB100-378-2) used for IP at 6 ug/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot NB100-378-1) of this antibody. For blotting immunoprecipitated CKII alpha, NB100-378 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunoprecipitation-NB100-378-img0013.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0022.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0016.jpg)
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (S05-7F8) [NBP3-19853] - Western blot detection of Casein Kinase 2 alpha in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), mouse Brain (Lane 4), Ramos (Lane 5) using NBP3-19853 (1:1000 diluted)](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-S05-7F8-Western-Blot-NBP3-19853-img0001.jpg "Western Blot: Casein Kinase 2 alpha Antibody (S05-7F8) [NBP3-19853] - Western blot detection of Casein Kinase 2 alpha in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), mouse Brain (Lane 4), Ramos (Lane 5) using NBP3-19853 (1:1000 diluted)")
![SDS-Page: Recombinant Human Casein Kinase 2 alpha Protein [NBP1-99066] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Casein-Kinase-2-alpha-Protein-SDS-Page-NBP1-99066-img0001.jpg "SDS-Page: Recombinant Human Casein Kinase 2 alpha Protein [NBP1-99066] - 15% SDS-PAGE (3ug)")
![Western Blot: Casein Kinase 2 alpha Antibody (2P3M7) [NBP3-15858] - Western blot analysis of various lysates, using Casein Kinase 2 alpha antibody at 1:1000 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15858_rabbit-casein-kinase-2-alpha-mab-2p3m7-7220251245377.jpg "Western Blot: Casein Kinase 2 alpha Antibody (2P3M7) [NBP3-15858] - Western blot analysis of various lysates, using Casein Kinase 2 alpha antibody at 1:1000 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody (2P3M7) [NBP3-15858] - Western blot analysis of extracts from wild type(WT) and Casein Kinase 2 alpha knockdown (KD) 293T cells, using Casein Kinase 2 alpha antibody at 1:1000 dilution.<br>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15858_rabbit-casein-kinase-2-alpha-mab-2p3m7-72202512432021.jpg)
![Immunocytochemistry/Immunofluorescence: Casein Kinase 2 alpha Antibody [NBP2-57124] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57124-img0001.jpg "Immunocytochemistry/Immunofluorescence: Casein Kinase 2 alpha Antibody [NBP2-57124] - Staining of human cell line A-431 shows localization to nucleoplasm.")
