Products Related to Gene-ID: 11082

NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="Endocan/ESM-1 was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

1999-EC) was serially diluted and captured by Rat Anti-Mouse Endocan/ESM‑1 Monoclonal Antibody (Catalog # MAB1999) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1999-EC) was serially diluted and captured by Rat Anti-Mouse Endocan/ESM‑1 Monoclonal Antibody (Catalog # MAB1999) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF017). A specific band was detected for Endocan/ESM-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Endocan/ESM-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endocan/ESM-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1810-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM-1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 1810-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM-1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

1999-EC), immo-bilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM-1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Endocan/ESM-1 Monoclonal Antibody (Catalog # MAB1999). The ND₅₀ is typically 0.75-3.5 µg/mL." class="big_lightbox"> 1999-EC), immo-bilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM-1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Endocan/ESM-1 Monoclonal Antibody (Catalog # MAB1999). The ND₅₀ is typically 0.75-3.5 µg/mL." alt="Recombinant Mouse Endocan/ESM-1 (Catalog # 1999-EC), immo-bilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM-1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Endocan/ESM-1 Monoclonal Antibody (Catalog # MAB1999). The ND₅₀ is typically 0.75-3.5 µg/mL." class="big_thumb" />

1999-EC) was serially diluted and captured by Rat Anti-Mouse Endocan/ESM‑1 Monoclonal Antibody (Catalog # MAB1999) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1999-EC) was serially diluted and captured by Rat Anti-Mouse Endocan/ESM‑1 Monoclonal Antibody (Catalog # MAB1999) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />