NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1226-SP | |||
FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27773-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11871-0.025mg | |||
NL013) and counterstained with DAPI (blue). View our protocol for ![Western Blot: Ly-6A/E Antibody (SR3456) [NBP3-27773] - Western blot analysis of mouse kidney lysate.](http://images.novusbio.com/fullsize/nbp3-27773_rabbit-ly-6a-e-mab-sr3456-7520249391317.jpg "Western Blot: Ly-6A/E Antibody (SR3456) [NBP3-27773] - Western blot analysis of mouse kidney lysate.")
![Western Blot: Ly-6A/E Antibody (4N5K2) [NBP3-15949] - Western blot analysis of extracts of various cell lines, using Ly-6A/E antibody (NBP3-15949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Ly-6A-E-Antibody-4N5K2-Western-Blot-NBP3-15949-img0001.jpg "Western Blot: Ly-6A/E Antibody (4N5K2) [NBP3-15949] - Western blot analysis of extracts of various cell lines, using Ly-6A/E antibody (NBP3-15949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
