| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13683-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22562-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57627-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97442-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22560-50ul | |||
![Western Blot: NUP50 Antibody [NB100-93324] - MDA-MB-231 nuclear extract. Cells infected with lentivirus coding scramble shRNA or one of two NUP50 targeting shRNAs. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NB100-93324-img0006.jpg "Western Blot: NUP50 Antibody [NB100-93324] - MDA-MB-231 nuclear extract. Cells infected with lentivirus coding scramble shRNA or one of two NUP50 targeting shRNAs. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-93324] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NUP50 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-NB100-93324-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NUP50 Antibody [NB100-93324] - Detection of Human NUP50 on HeLa whole cell lysate using NB100-93324. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody NB100-93323.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NB100-93324-img0002.jpg)
![Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NB100-1212-img0005.jpg "Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212] - 3.8ug/ml staining of Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NB100-1212-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19610-img0004.jpg "Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-19610-img0003.jpg)
![Western Blot: NUP50 Antibody [NBP2-19610] - 50 ug mouse testis lysate/extract 10% SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NBP2-19610-img0001.jpg)
![Western Blot: NUP50 Antibody [NBP2-19610] -Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19610_rabbit-polyclonal-nup50-antibody-western-blot-2982024114447..jpg)
![Western Blot: NUP50 Antibody [NBP2-19610] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (NBP2-19610) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19610_rabbit-polyclonal-nup50-antibody-910202420122012.jpg)
![Western Blot: NUP50 Antibody [NBP2-13683] - Analysis using Anti-NUP50 antibody NBP2-13683 (A) shows similar pattern to independent antibody NBP2-57627 (B).](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NBP2-13683-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NUP50 Antibody [NBP2-13683] - Analysis using Anti-NUP50 antibody NBP2-13683 (A) shows similar pattern to independent antibody NBP2-57627 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-13683] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13683-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining in human testis and heart muscle tissues using anti-NUP50 antibody. Corresponding NUP50 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-13683-img0008.jpg)
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining of human heart muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-13683-img0006.jpg)
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-13683-img0007.jpg)
![Western Blot: NUP50 Antibody (SR1992) [NBP3-22562] - Western blot analysis using NBP3-22562 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22562_rabbit-nup50-mab-sr1992-57202315584831.jpg "Western Blot: NUP50 Antibody (SR1992) [NBP3-22562] - Western blot analysis using NBP3-22562 in HeLa cell lysate.")
![Western Blot: NUP50 Antibody (JE64-06) [NBP3-32671] - Western blot analysis of NUP50 on different lysates with Rabbit anti-NUP50 antibody (NBP3-32671) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32671) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32671_rabbit-nup50-mab-je64-06-278202416285296.jpg "Western Blot: NUP50 Antibody (JE64-06) [NBP3-32671] - Western blot analysis of NUP50 on different lysates with Rabbit anti-NUP50 antibody (NBP3-32671) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32671) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUP50 Antibody (JE64-06) [NBP3-32671] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-NUP50 antibody (NBP3-32671) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32671) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32671_rabbit-nup50-mab-je64-06-29820241435229.jpg)
![Western Blot: NUP50 Antibody [NBP2-57627] - Analysis using Anti-NUP50 antibody NBP2-57627 (A) shows similar pattern to independent antibody NBP2-13683 (B).](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NBP2-57627-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NUP50 Antibody [NBP2-57627] - Analysis using Anti-NUP50 antibody NBP2-57627 (A) shows similar pattern to independent antibody NBP2-13683 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-57627] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57627-img0001.jpg)
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-97442] - Immunochemical staining of human NUP50 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-97442-img0003.jpg "Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-97442] - Immunochemical staining of human NUP50 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-97442] - Immunochemical staining of human NUP50 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-97442-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-97442] - Immunofluorescence staining of NUP50 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97442 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97442_rabbit-polyclonal-nup50-antibody-235202511254417.png)
![Western Blot: NUP50 Antibody (SR1991) [NBP3-22560] - Western blot analysis using NBP3-22560 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22560_rabbit-nup50-mab-sr1991-57202315545141.jpg "Western Blot: NUP50 Antibody (SR1991) [NBP3-22560] - Western blot analysis using NBP3-22560 in HeLa cell lysate.")
![Recombinant Human NUP50 Protein [H00010762-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010762-P01-1.jpg "Recombinant Human NUP50 Protein [H00010762-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: NUP50 Antibody (4H7) [H00010762-M01] - NUP50 monoclonal antibody (M01), clone 4H7. Analysis of NUP50 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/NUP50-Antibody-4H7-Western-Blot-H00010762-M01-img0002.jpg "Western Blot: NUP50 Antibody (4H7) [H00010762-M01] - NUP50 monoclonal antibody (M01), clone 4H7. Analysis of NUP50 expression in Hela S3 NE.")
![Sandwich ELISA: NUP50 Antibody (4H7) [H00010762-M01] - Detection limit for recombinant GST tagged NUP50 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NUP50-Antibody-4H7-Sandwich-ELISA-H00010762-M01-img0001.jpg)
![Western Blot: NUP50 Overexpression Lysate (Adult Normal) [NBL1-13878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUP50.](http://images.novusbio.com/fullsize/NUP50-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13878-img0002.jpg "Western Blot: NUP50 Overexpression Lysate (Adult Normal) [NBL1-13878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUP50.")