| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73338 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5460-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5460-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99498-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20106-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20105-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17000-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03705-20ul | |||
![Western Blot: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - EK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peroxiredoxin-4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Peroxiredoxin-4.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Western-Blot-NBP2-02117-img0018.jpg "Western Blot: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - EK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peroxiredoxin-4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Peroxiredoxin-4.")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (OTI9A3) [NBP2-02117] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Peroxiredoxin-4.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunocytochemistry-Immunofluorescence-NBP2-02117-img0010.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Human prostate tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0017.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0011.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Human breast tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0012.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Human colon tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0013.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Human endometrium tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0014.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Human Ovary tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0015.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (9A3) [NBP2-02117] - Staining of paraffin-embedded Human pancreas tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9A3-Immunohistochemistry-Paraffin-NBP2-02117-img0016.jpg)
![Western Blot: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peroxiredoxin-4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Peroxi](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Western-Blot-NBP2-73338-img0001.jpg "Western Blot: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peroxiredoxin-4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Peroxi")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Peroxiredoxin-4.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73338-img0002.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Human pancreas tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0008.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0003.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Human breast tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0004.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Human colon tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0005.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Human endometrium tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0006.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Human Ovary tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0007.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (OTI9A3) - Azide and BSA Free [NBP2-73338] - Staining of paraffin-embedded Human prostate tissue using anti-Peroxiredoxin-4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-OTI9A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73338-img0009.jpg)
![Western Blot: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 4 (PRDX4) Rabbit mAb (NBP3-16760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9E7V0-Western-Blot-NBP3-16760-img0005.jpg "Western Blot: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 4 (PRDX4) Rabbit mAb (NBP3-16760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunofluorescence analysis of NIH-3T3 cells using Peroxiredoxin 4 (PRDX4) Rabbit mAb (NBP3-16760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-9E7V0-Immunocytochemistry-Immunofluorescence-NBP3-16760-img0004.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded human cervix cancer tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025208499.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded mouse testis tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-8220252010498.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunofluorescence analysis of NIH-3T3 cells using Peroxiredoxin 4 (PRDX4) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025209890.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded human liver tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025208452.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded human thyroid cancer tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025209810.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded human lung cancer tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025209893.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded rat colon tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025209851.jpg)
![Immunohistochemistry: Peroxiredoxin 4 Antibody (9E7V0) [NBP3-16760] - Immunohistochemistry analysis of Peroxiredoxin 4 in paraffin-embedded human colon carcinoma tissue using Peroxiredoxin 4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16760_rabbit-peroxiredoxin-4-mab-9e7v0-822025209841.jpg)
![Western Blot: Peroxiredoxin 4 Antibody [NBP2-19778] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cellsHeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (oncotarget.com/abstract/7142), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Western-Blot-NBP2-19778-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Peroxiredoxin 4 Antibody [NBP2-19778] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cellsHeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (oncotarget.com/abstract/7142), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin-4 Antibody [NBP2-19778] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-19778-img0003.jpg)
![Western Blot: Peroxiredoxin-4 Antibody [NBP2-19778] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Western-Blot-NBP2-19778-img0001.jpg)
![Western Blot: Peroxiredoxin-4 Antibody [NBP2-19778] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Western-Blot-NBP2-19778-img0002.jpg)
![Western Blot: Peroxiredoxin 4 Antibody [NBP2-19778] - Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha , HIF-2 alpha , V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19778_rabbit-polyclonal-peroxiredoxin-4-antibody-310202415334941.jpg)
![Western Blot: Peroxiredoxin 4 Antibody [NBP2-99498] - Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Western-Blot-NBP2-99498-img0007.jpg "Western Blot: Peroxiredoxin 4 Antibody [NBP2-99498] - Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa")
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP2-99498] - Immunochemical staining of human Peroxiredoxin 4 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-99498-img0005.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP2-99498] - Immunochemical staining of human Peroxiredoxin 4 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-99498-img0004.jpg)
![Immunoprecipitation: Peroxiredoxin 4 Antibody [NBP2-99498] - Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PRDX4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody:Anti-PRDX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDaObserved band size :28 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunoprecipitation-NBP2-99498-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody [NBP2-99498] - Immunofluorescence staining of Peroxiredoxin 4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99498 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99498_rabbit-polyclonal-peroxiredoxin-4-antibody-235202511545640.png)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-40C-8 (NBP3-20106) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20106, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20106_rabbit-peroxiredoxin-4-mab-saic-40c-8-azide-and-bsa-free-2742023924227.png "Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-40C-8 (NBP3-20106) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20106, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-40C-8 (NBP3-20106, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20106_rabbit-peroxiredoxin-4-mab-saic-40c-8-azide-and-bsa-free-2742023924260.png)
![Western Blot: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - HEK293 cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40C-8 (NBP3-20106) at 0.001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20106_rabbit-peroxiredoxin-4-mab-saic-40c-8-azide-and-bsa-free-2742023924214.png)
![Western Blot: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Human liver(A) and human pancreas(B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40A-24 (NBP3-20105) at 0.001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20105_rabbit-peroxiredoxin-4-mab-saic-40a-24-azide-bsa-free-2742023924229.png "Western Blot: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Human liver(A) and human pancreas(B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40A-24 (NBP3-20105) at 0.001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-40A-24 (NBP3-20105) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20105, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20105_rabbit-peroxiredoxin-4-mab-saic-40a-24-azide-bsa-free-2742023924211.png)
![Flow Cytometry: Peroxiredoxin 4 Antibody (SAIC-40A-24) - Azide and BSA Free [NBP3-20105] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-40A-24 (NBP3-20105, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20105_rabbit-peroxiredoxin-4-mab-saic-40a-24-azide-bsa-free-2742023924233.png)
![Western Blot: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Western blot analysis of Peroxiredoxin 4 on different lysates with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: LNCaP cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 27 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32739) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32739_rabbit-peroxiredoxin-4-mab-psh03-40-278202415242644.jpg "Western Blot: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Western blot analysis of Peroxiredoxin 4 on different lysates with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: LNCaP cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 27 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32739) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32739) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32739_rabbit-peroxiredoxin-4-mab-psh03-40-29820241492639.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 4 with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32739_rabbit-peroxiredoxin-4-mab-psh03-40-2920241114812.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP3-17000] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-17000-img0002.jpg "Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP3-17000] - Staining of human cerebral cortex shows low expression as expected.")
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP3-17000] - Analysis in human pancreas and cerebral cortex tissues using Anti-PRDX4 antibody. Corresponding PRDX4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-17000-img0001.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP3-17000] - Staining of human pancreas shows high expression.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-17000-img0003.jpg)
![Western Blot: Peroxiredoxin 4 Antibody [NBP3-03705] - Analysis of extracts of various cell lines, using Peroxiredoxin 4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Western-Blot-NBP3-03705-img0002.jpg "Western Blot: Peroxiredoxin 4 Antibody [NBP3-03705] - Analysis of extracts of various cell lines, using Peroxiredoxin 4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 4 Antibody [NBP3-03705] - Analysis of extracts from normal (control) and PRDx4 knockout (KO) 293T cells, using Peroxiredoxin 4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Peroxiredoxin-4-Antibody-Knockout-Validated-NBP3-03705-img0001.jpg)