The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for checking the even loading of gel samples, and the even transfer of proteins at the blotting stage. They also serve a purpose in quality control, by verifying reagents are working correctly, and in the standardization of experimental results.
One of six actin isoforms so far identified, beta actin is a highly conserved cytoskeletal protein involved in cell structure, motility, and cohesion. We at Novus Biologicals have many different beta-actin products in our catalog, used in areas such as cytoskeleton and signal transduction research. They are also used as loading controls in protein assays. Beta-actin is known as a “housekeeping” protein, i.e.
In rodents, MAP1LC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form, LC3II, following conversion from LC3I.
