Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB100-56343AFP488 | |||
| Alexa Fluor Plus 555 | NB100-56343AFP555 | |||
| Alexa Fluor Plus 594 | NB100-56343AFP594 | |||
| Alexa Fluor Plus 647 | NB100-56343AFP647 | |||
| Alexa Fluor Plus 680 | NB100-56343AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56343SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-16773 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5407-SP | |||
![Western Blot: EFTUD2 Antibody [NB100-40849] - a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Knockdown-Validated-NB100-40849-img0006.jpg "<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: EFTUD2 Antibody [NB100-40849] - a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry: EFTUD2 Antibody [NB100-40849] - Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2. Representative IHC images from 50 human HCC tissue samples. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-03040-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-NB100-40849-img0007.jpg)
![Immunohistochemistry-Paraffin: EFTUD2 Antibody [NB100-40849] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-EFTUD2/SNRP116 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunohistochemistry-Paraffin-NB100-40849-img0005.jpg)
![Immunoprecipitation: EFTUD2 Antibody [NB100-40849] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti- EFTUD2/SNRP116 antibody 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/fullsize/EFTUD2-Antibody-Immunoprecipitation-NB100-40849-img0003.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 expression is necessary for HCC cell survival.a The protein & mRNA levels of EFTUD2 expression in HCC cells detected by western blot & qPCR. b Western blot analysis of EFTUD2 expression in Hep3B & Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). c. d The effect of EFTUD2 on cellular viability was detected by CCK8 assays & colony formation assays, respectively. e Western blot analysis of EFTUD2 protein expression in Hep3B & Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). f Representative images show the cell morphological changes after the stable knockdown of EFTUD2. Scale bars, 100 μm. g, h CCK8 assays & colony formation assays show the effect of EFTUD2 on cellular survival in Hep3B & Huh7 cells with a stable knockdown of EFTUD2. The data are presented as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-310202415541961.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 promotes EMT via activation of the STAT3 pathway in HCC cells.a An M–A plot showed the genes differentially expressed between EFTUD2-overexpressing & control Hep G2 cells (N = 3), with the differentially regulated genes highlighted in red. b Gene sets of the IL6_JAK_STAT3 signaling pathway & the EMT were enriched in EFTUD2-overexpressing Hep G2 cells. c Serial sections of the xenograft tumors were subjected to IHC staining of EFTUD2, pSTAT3, E-cadherin & vimentin. Scale bars, 100 μm. d Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2 & pSTAT3. Representative IHC images were showed in the left panel, & the right panel showed the correlation between EFTUD2 & pSTAT3 expression, in 50 human HCC tissue samples. Scale bars, 100 μm. e The expression of EFTUD2, vimentin, Twist1, E-cadherin, STAT3, pSTAT3, MCL-1, & GAPDH was detected by western blotting in the indicated cells. f Western blot analysis showing that the increases in pSTAT3 & vimentin expression were compromised & that the decrease in E-cadherin was reversed by SI3-201, a STAT3 inhibitor, in EFTUD2-overexpressing Hep G2 cells. g The Hep G2 cells with EFTUD2 overexpression were treated with 100 μM SI3-201 & subjected to migration & invasion assays. Scale bars, 200 μm. The data are presented as the mean ± SD from triplicate independent experiments, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-310202415525297.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 promotes EMT via activation of the STAT3 pathway in HCC cells.a An M–A plot showed the genes differentially expressed between EFTUD2-overexpressing & control Hep G2 cells (N = 3), with the differentially regulated genes highlighted in red. b Gene sets of the IL6_JAK_STAT3 signaling pathway & the EMT were enriched in EFTUD2-overexpressing Hep G2 cells. c Serial sections of the xenograft tumors were subjected to IHC staining of EFTUD2, pSTAT3, E-cadherin & vimentin. Scale bars, 100 μm. d Serial sections of human HCC tissue samples were subjected to IHC staining with antibodies against EFTUD2 & pSTAT3. Representative IHC images were showed in the left panel, & the right panel showed the correlation between EFTUD2 & pSTAT3 expression, in 50 human HCC tissue samples. Scale bars, 100 μm. e The expression of EFTUD2, vimentin, Twist1, E-cadherin, STAT3, pSTAT3, MCL-1, & GAPDH was detected by western blotting in the indicated cells. f Western blot analysis showing that the increases in pSTAT3 & vimentin expression were compromised & that the decrease in E-cadherin was reversed by SI3-201, a STAT3 inhibitor, in EFTUD2-overexpressing Hep G2 cells. g The Hep G2 cells with EFTUD2 overexpression were treated with 100 μM SI3-201 & subjected to migration & invasion assays. Scale bars, 200 μm. The data are presented as the mean ± SD from triplicate independent experiments, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-31020241683938.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - Depletion of EFTUD2 blocks cell cycle progression & promotes cell apoptosis.a EdU incorporation assays were used to identify the cells in S phase after EFTUD2 silencing in the indicated cells. Red: cell in S phase. Scale bars, 200 μm. b The cell cycle analyses were performed in Hep3B & Huh7 cells with EFTUD2 knockdown. c, d Fluorescence microscopy & flow cytometry were performed to captured early apoptotic cells by mitochondrial membrane potential changes in Hep3B & Huh7 cells with EFTUD2 knockdown. Red, JC-1 aggregates; green, JC-1 monomers. Scale bars, 10 μm. e, f EFTUD2-silenced Hep3B & Huh7 cells treated with or without CDDP (2 μg/mL; 48 h) were stained with a combination of Annexin V-FITC & PI & analyzed by FACS. Cells positive for FITC staining were counted as apoptotic cells. The bar graphs show the percentage of apoptotic cells. g Apoptosis-associated proteins of the indicated cells were measured by western blotting. The data are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-310202415533811.jpg)
![Western Blot: EFTUD2 Antibody [NB100-40849] - EFTUD2 promotes HCC growth.a Western blot analysis of EFTUD2 protein expression in Hep G2 cells infected with the EFTUD2 overexpression lentivirus or the vector lentivirus. b, c The effect of EFTUD2 overexpression on cell proliferation was detected by CCK8 assays & colony formation assays. d EdU incorporation assays were used to identify the cells in S phase. e The distribution of different cell cycle phases in the indicated cells. f Tumor volumes were measured & recorded every 3 days, & a growth curve was plotted. g Subcutaneous xenograft tumors of the indicated cells. The right panel shows the wet weights of the xenograft tumors. h Representative images of H&E & IHC staining of EFTUD2 & Ki67 in the xenograft tumors. Scale bars, 100 μm. The data are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; NS no significance. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33024090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40849_rabbit-polyclonal-eftud2-antibody-31020241554528.jpg)
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Western Blot: CIZ1 Antibody [NB100-74624] - Detection of Human CIZ1on HeLa whole cell lysate using NB100-74624. CIZ1 was also immunoprecipitated by rabbit anti-CIZ1 antibody NB100-74623.](http://images.novusbio.com/fullsize/CIZ1-Antibody-Western-Blot-NB100-74624-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CIZ1 Antibody [NB100-74624] - Detection of Human CIZ1on HeLa whole cell lysate using NB100-74624. CIZ1 was also immunoprecipitated by rabbit anti-CIZ1 antibody NB100-74623.")
![Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC5 antibody NB100-469 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMC5-Antibody-Western-Blot-NB100-469-img0001.jpg "Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC5 antibody NB100-469 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: SMC5 Antibody [NB100-469] - Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC5 antibody NB100-469 (lot 3) used for IP at 6 ug per reaction. SMC5 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated SMC5, NB100-469 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/SMC5-Antibody-Immunoprecipitation-NB100-469-img0002.jpg)
![Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0005.jpg "Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0001.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0003.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0004.jpg)
![Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NBP2-21581-img0017.jpg "Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.")
![Flow Cytometry: HA Tag Antibody [NBP2-21581] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Flow-Cytometry-NBP2-21581-img0013.jpg)
![Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-12793-img0002.jpg "Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.")
![Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NBP2-12793-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31733-img0020.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-31733-img0015.jpg)
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0017.jpg)
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0019.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-31733-img0014.jpg)
![Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using MYH9 antibody [N1-2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunoprecipitation-NBP1-31733-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31733_rabbit-polyclonal-non-muscle-myosin-iia-antibody-1610202419405784.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [N1-2], N-term (NBP1-31733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31733_rabbit-polyclonal-non-muscle-myosin-iia-antibody-western-blot-1411202518354619.jpg)
![Western Blot: DCK Antibody [NBP2-16108] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-Western-Blot-NBP2-16108-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DCK Antibody [NBP2-16108] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: DCK Antibody [NBP2-16108] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-Western-Blot-NBP2-16108-img0014.jpg)
![Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108] - Rat lung. DCK stained by DCK antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DCK-Antibody-Immunohistochemistry-Paraffin-NBP2-16108-img0013.jpg)
![Western Blot: DCK Antibody [NBP2-16108] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-Western-Blot-NBP2-16108-img0005.jpg)
![Western Blot: DCK Antibody [NBP2-16108] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12% SDS-PAGE DCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-Western-Blot-NBP2-16108-img0012.jpg)
![Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108] - Mouse hind brain. DCK antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DCK-Antibody-Immunohistochemistry-Paraffin-NBP2-16108-img0010.jpg)
![Immunoprecipitation: DCK Antibody [NBP2-16108] - Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 ug of DCK antibody. Western blot analysis was performed using DCK antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/DCK-Antibody-Immunoprecipitation-NBP2-16108-img0006.jpg)
![Western Blot: DCK Antibody [NBP2-16108] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (NBP2-16108) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16108_rabbit-polyclonal-dck-antibody-91020242063395.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43720_rabbit-polyclonal-mcherry-antibody-9102024205672.jpg "Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunoprecipitation: mCherry Antibody [NBP2-43720] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunoprecipitation-NBP2-43720-img0002.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Analysis of A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12 % SDS-PAGE mCherry antibody dilution: 1:2000.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-43720-img0004.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43720_rabbit-polyclonal-mcherry-antibody-91020242014626.jpg)
