Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB918U-100UG | |||
| Alexa Fluor 405 | FAB918V-100UG | |||
| Alexa Fluor 488 | FAB918G-100UG | |||
| Alexa Fluor 532 | FAB918X-100UG | |||
| Alexa Fluor 594 | FAB918T-100UG | |||
| Alexa Fluor 647 | FAB918R-100UG | |||
| Alexa Fluor 700 | FAB918N-100UG | |||
| Alexa Fluor 750 | FAB918S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB918-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-27946SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56135SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26221-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1108-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-599SS | |||
| Azide and BSA Free | NBP2-80601 | |||
![Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SFPQ-Antibody-Immunohistochemistry-Paraffin-NBP2-20329-img0003.jpg "Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded mouse uterus, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SFPQ-Antibody-Immunohistochemistry-Paraffin-NBP2-20329-img0004.jpg)
![Western Blot: SFPQ Antibody [NBP2-20329] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-910202420115084.jpg)
![SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:500. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-immunohistochemistry-paraffin-261120251965224.jpg)
![SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-immunocytochemistry-immunofluorescence-261120251847423.jpg)
![SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal (NBP2-20329) diluted at 1:500. br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20329_rabbit-polyclonal-sfpq-antibody-immunohistochemistry-paraffin-261120251824169.jpg)
![Immunohistochemistry-Frozen: Cre Antibody [NB100-56135] - Cochlea cross-section of Cre+/-mouse, stained for Cre (green) and counterstained for F-actin (red). Tissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-Cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum. Image from verified customer review.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-Frozen-NB100-56135-img0002.jpg "Immunohistochemistry-Frozen: Cre Antibody [NB100-56135] - Cochlea cross-section of Cre+/-mouse, stained for Cre (green) and counterstained for F-actin (red). Tissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-Cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Cre Antibody [NB100-56135] - Analysis of tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. Arrows point to negative glia.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56135-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Lyn Antibody [NBP2-17188] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17188-img0003.jpg "Immunocytochemistry/Immunofluorescence: Lyn Antibody [NBP2-17188] - Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using LYN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: Lyn Antibody [NBP2-17188] - Human colon carcinoma, using LYN antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Lyn-Antibody-Immunohistochemistry-Paraffin-NBP2-17188-img0002.jpg)
![Western Blot: Lyn Antibody [NBP2-17188] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Lyn antibody (NBP2-17188) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17188_rabbit-polyclonal-lyn-antibody-910202420122030.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Lyn Antibody [NBP2-17188] - Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17188_rabbit-polyclonal-lyn-antibody-1610202419405711.jpg)
![LYN antibody immunoprecipitates LYN protein in IP experiments.IP samples: K562 whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of LYN protein by 4 ug LYN antibody (NBP2-17188)5 % SDS-PAGEThe immunoprecipitated LYN protein was detected by LYN antibody (NBP2-17188) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17188_rabbit-polyclonal-lyn-antibody-immunoprecipitation-2611202518321918.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011413.jpg "Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Flow Cytometry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Overlay Peak curve showing MCF7 cells stained with NBP3-26221 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101142.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of Hela cell with NBP3-26221 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101149.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011417.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of HepG2 cell with NBP3-26221 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011412.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101145.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101144.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011415.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF-1 alpha and BICD1. Cells were pretreated with MG132 (1 uM) for 30 min prior to hypoxia treatment for 24 h. Co-immunoprecipitation of HIF-1 alpha with IgG and BICD1 were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-018-0241-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0004.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Role of BICD1 in UCB-MSCs survival under hypoxia. a, b The UCB-MSCs were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment. a Cells were exposed to various durations of hypoxia (0–72 h). Cell viability of UCB-MSCs was measured by WST-1 cell viability assay. n = 8. b Cells were incubated in normoxia or hypoxia conditions for 72 h. Representative images of experimental groups at 0, 24, 48, & 72 h of normoxia or hypoxia incubation are presented. Red-marked cells in representative images indicate PI-positive cells. n = 4. Scale bars are 50 μm (Magnification, × 200). cBICD1 or NT siRNA-transfected cells were incubated in hypoxia for 24 h. Cleaved caspase-9, cleaved caspase-3, & beta -Actin protein expressions were detected by western blot analysis. n = 4. d Cells were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 48 h. The percentages of apoptotic cells were analyzed by Annexin V/PI analysis, measured by flowcytometer. Annexin V-positive cells were considered as apoptotic cells. n = 4. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection. e The UCB-MSCs were transfected with pcDNA3.1/BICD1-cEGFP vector, pcDNA3.1/cEGFP vector, HIF1A siRNA or NT siRNA for 24 h prior to hypoxia treatment for 72 h. Cell viability data are presented as a mean ± S.E.M. n = 5. *p < 0.05 vs. normoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, #p < 0.05 vs. hypoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, @p < 0.05 vs. hypoxia control with pcDNA3.1/BICD1-cEGFP vector & NT siRNA cotransfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171413.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171451.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616184.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Involvement of Akt/GSK3 beta pathway in BICD1-mediated HIF1 alpha nuclear translocation. a–e The UCB-MSCs were transfected with GSK3 beta or NT siRNA for 24 h prior to hypoxia treatment for 24 h. a Co-immunoprecipitation of HIF1 alpha & Dynein IC with IgG & BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. b Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) was analyzed by PLA assay. Scale bars are 8 μm (Magnification, × 1,000). n = 5. c HIF1 alpha , Lamin A/C, & alpha -Tubulin in cytosolic & nuclear fractionized samples were detected by western blot. n = 4. d Cells were immunostained with HIF1 alpha -specific antibody. Scale bars are 8 μm (Magnification, × 1,000). n = 4. e HIF1 activities in NT or GSK3 beta siRNA-transfected cells were analyzed by dual luciferase reporter assay. n = 6. Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616183.jpg)
![Western Blot: CDT1 Antibody [NB100-2567] - Human B cells. KSHV infection protects CDT1 from hypoxia mediated degradation. Mock or KSHV infected cells were grown under normoxic or hypoxic condition followed by measuring CDT1 levels. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CDT1-Antibody-Western-Blot-NB100-2567-img0005.jpg "Western Blot: CDT1 Antibody [NB100-2567] - Human B cells. KSHV infection protects CDT1 from hypoxia mediated degradation. Mock or KSHV infected cells were grown under normoxic or hypoxic condition followed by measuring CDT1 levels. Western blot image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: CDT1 Antibody [NB100-2567] - Human islet cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/mL).](http://images.novusbio.com/fullsize/CDT1-Antibody-Immunohistochemistry-Paraffin-NB100-2567-img0004.jpg)
![Western Blot: CDT1 Antibody [NB100-2567] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti- CDT1 antibody (NB100-2567) used for WB at 1 ug/mL (A and B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/fullsize/CDT1-Antibody-Western-Blot-NB100-2567-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunocytochemistry-immunofluorescence-8112023132213..jpg "Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Analysis of paraffin-embedded sections of zebrafish kidney tube, using FXR1 antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132323..jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Mouse kidney.FXR1 stained by FXR1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132436..jpg)
![Western Blot: FXR1 Antibody [NBP1-33719] - Sample (30 ug of whole cell lysate) A: zebrafish brain 7.5% SDS PAGE NBP1-33719 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-33719_rabbit-polyclonal-fxr1-antibody-910202420134079.jpg)
![Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Western-Blot-NB100-599-img0008.jpg "Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.")
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (KEN) [NB100-599] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BRCA1 (KEN) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Immunocytochemistry-Immunofluorescence-NB100-599-img0009.jpg)
![Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Western-Blot-NB100-599-img0005.jpg)
![Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Western-Blot-NBP1-33188-img0008.jpg "Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SerpinB2 Antibody [NBP1-33188] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33188-img0012.jpg)
![Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Immunohistochemistry-Paraffin-NBP1-33188-img0004.jpg)
![Immunoprecipitation: SerpinB2 Antibody [NBP1-33188] - Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 ug of PAI2 antibody. Western blot analysis was performed using PAI2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SerpinB2-Antibody-Immunoprecipitation-NBP1-33188-img0010.jpg)
![Western Blot: SerpinB2 Antibody [NBP1-33188] -Sample (30 μg of whole cell lysate)A: U87-MG10% SDS PAGEdiluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33188_rabbit-polyclonal-serpinb2-antibody-western-blot-2410202411427..png)
![Western Blot: SerpinB2 Antibody [NBP1-33188] -Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp1-33188_rabbit-polyclonal-serpinb2-antibody-western-blot-2410202411643..png)