Cre Antibody

Images

 
Immunohistochemistry-Frozen: Cre Antibody [NB100-56135] - Cochlea cross-section of Cre+/-mouse, stained for Cre (green) and counterstained for F-actin (red). Tissue was fixed in 4% paraformaldehyde and incubated ...read more
Immunohistochemistry-Paraffin: Cre Antibody [NB100-56135] - Analysis of tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the ...read more

Product Details

Summary
Reactivity CsSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cre Antibody Summary

Immunogen
Full-length recombinant bacteriophage protein CRE was used as immunogen, GenBank no. AAQ14086.1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
WB: 1:1000-1:2000; IHC (paraffin): 1:1000-1:5000; IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents); IP: 1:50-1:200.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-56135 in the following applications:

Publications
Read Publications using
NB100-56135 in the following applications:

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Cre Antibody

  • Cyclization recombinase

Background

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells. This antibody recognizes the recombinase protein, Cre. Cre is a 343 amino acid protein, GenBank no. AAQ14086.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Cre Antibody (NB100-56135)(2)

Reviews for Cre Antibody (NB100-56135) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB100-56135:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Cre NB100-56135
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4
reviewed by:
Anonymous
IHC-Fr Mouse 08/27/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse inner ear
SpeciesMouse
LotAR60A

Comments

CommentsTissue was fixed in 4% paraformaldehyde and incubated overnight with the anti-cre antibody 1:100 in PBS containing Triton X-100 0.1% and 10% fetal calf serum
  5
reviewed by:
Anonymous
ICC Mouse 04/02/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested Mouse brain,Mouse embryo,Transgenic Mouse Brain (40um)
SpeciesMouse
LotN/A

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cre Antibody (NB100-56135). (Showing 1 - 1 of 1 FAQs).

  1. A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
    • With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Cre Products

Array NB100-56135

Blogs on Cre.

Insulin signaling in brain’s subfornical organ is crucial for regulating cardiometabolic profile
By Jamshed Arslan, Pharm. D., PhD. The hypothalamus is an insulin receptor-rich brain region. Insulin receptor signaling in the CNS can regulate blood pressure, for example, by increasing sympathetic outflow to th...  Read full blog post.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

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Recent Reviews

4.5
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Anonymous
08/27/2018
Application: IHC-Fr
Species: Mouse

 
Anonymous
04/02/2017
Application: ICC
Species: Mouse