Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-744-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-747-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF935-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1294-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-602AFP488 | |||
| Alexa Fluor Plus 555 | NB600-602AFP555 | |||
| Alexa Fluor Plus 594 | NB600-602AFP594 | |||
| Alexa Fluor Plus 647 | NB600-602AFP647 | |||
| Alexa Fluor Plus 680 | NB600-602AFP680 | |||
| HRP | NB120-7635-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3609-SP | |||
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining of Cytokeratin 4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining of Cytokeratin 4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0009.jpg)
![Flow Cytometry: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of A431 cells with Cytokeratin 4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Flow-Cytometry-NBP2-67706-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining Cytokeratin 4 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining Cytokeratin 4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining Cytokeratin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 4 (washed with PBS. And then probed with the primary antibodies Cytokeratin 4 ( red) at 1/200 dilution and Vimentin (green) at 1/200 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of paraffin-embedded human esophagus tissue using anti-Cytokeratin 4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0008.jpg)
![Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Western-Blot-NBP2-66978-img0003.jpg "Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-M antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0002.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0006.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using NB100-744 at a dilution of 1:200.](http://images.novusbio.com/fullsize/Cytosine-5-Methyl-Antibody-Immunocytochemistry-Immunofluorescence-NB100-744-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: 5-MethylCytosine Antibody [NB100-744] - Immunoblotting analysis shows decrease in 5-methyl cytosine and increase in H3K9 acetylation levels after combined treatment. Lanes 1 to 5 were loaded with proteins (2 ug) from HFFs, hESCs, inhibitor treated HFFs and combined treated HFFs. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB100-747] - Human Prostate 5 ug/ml.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB100-747-img0001.jpg "Immunohistochemistry-Paraffin: Histone H3 Antibody [NB100-747] - Human Prostate 5 ug/ml.")
![Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-Western-Blot-NB200-527-img0001.jpg "Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.")
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0012.jpg "Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (8A3) [NBP2-78825] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunohistochemistry-NBP2-78825-img0013.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Western-Blot-NBP2-78825-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 8A3 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-8A3-Immunoprecipitation-NBP2-78825-img0010.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0014.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.")
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0010.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0011.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0012.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NB600-602-img0013.jpg)
