Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52448-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59353-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37312-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52512-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52494-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96875-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75399 | |||
| Pre-adsorbed | NBP1-75401 | |||
| Pre-adsorbed | NBP1-75402 | |||
| Pre-adsorbed | NBP1-75398-0.5mg | |||
| Pre-adsorbed | NBP1-75398JF549 | |||
| Pre-adsorbed | NBP1-75398JF646 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-74975 | |||
| Biotin | NBP1-74976 | |||
| DyLight 488 | NBP1-74980 | |||
| DyLight 550 | NBP1-75816 | |||
| DyLight 594 | NBP1-75817 | |||
| DyLight 633 | NBP1-75818 | |||
| FITC | NBP1-74977 | |||
| HRP | NBP1-74978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05182-0.025mg | |||
![Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Western-Blot-NBP2-52448-img0004.jpg "Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)")
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Immunofluorescence-NBP2-52448-img0003.jpg)
![Flow Cytometry: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of A549 cells using CSF3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Flow-Cytometry-NBP2-52448-img0001.jpg)
![Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Western-Blot-NBP2-52448-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Immunofluorescence-NBP2-52448-img0002.jpg)
![ELISA: G-CSF Antibody (8G5F7) [NBP2-52448] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-ELISA-NBP2-52448-img0006.jpg)
![Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Western blot analysis using RBP4 mAb against HEK293 (1) and RBP4(AA: 1-201)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Western-Blot-NBP2-37614-img0002.jpg "Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Western blot analysis using RBP4 mAb against HEK293 (1) and RBP4(AA: 1-201)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Immunofluorescence analysis of HepG2 cells using RBP4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Immunocytochemistry-Immunofluorescence-NBP2-37614-img0003.jpg)
![Immunohistochemistry: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and stomach cancer tissues (right) using RBP4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Immunohistochemistry-NBP2-37614-img0005.jpg)
![Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Flow cytometric analysis of HepG2 cells using RBP4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Flow-Cytometry-NBP2-37614-img0004.jpg)
![ELISA: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-ELISA-NBP2-37614-img0001.jpg)
![Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (NBP2-59353) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/4-Hydroxynonenal-Antibody-12F7-Western-Blot-NBP2-59353-img0005.jpg "Western Blot: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (NBP2-59353) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7 (NBP2-59353). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (NBP2-59353) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/4-Hydroxynonenal-Antibody-12F7-Immunocytochemistry-Immunofluorescence-NBP2-59353-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: 4-Hydroxynonenal Antibody (12F7) [NBP2-59353] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/4-Hydroxynonenal-Antibody-12F7-Immunocytochemistry-Immunofluorescence-NBP2-59353-img0002.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0004.jpg "Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.")
![Immunohistochemistry: p38 Antibody (10B11C8) [NBP2-37312] - Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Immunohistochemistry-NBP2-37312-img0005.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0002.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0003.jpg)
![ELISA: p38 Antibody (10B11C8) [NBP2-37312] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-ELISA-NBP2-37312-img0001.jpg)
![Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Western-Blot-NBP2-52512-img0004.jpg "Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)")
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of bladder cancer tissues using MMP14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Immunohistochemistry-Paraffin-NBP2-52512-img0003.jpg)
![Flow Cytometry: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Flow-Cytometry-NBP2-52512-img0001.jpg)
![Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against HEK293 (1) and MMP14 (AA: 112-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Western-Blot-NBP2-52512-img0005.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of ovarian cancer tissues using MMP14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Immunohistochemistry-NBP2-52512-img0002.jpg)
![ELISA: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-ELISA-NBP2-52512-img0006.jpg)
![Western Blot: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Analysis using IL28A mouse mAb against SK-Br-3 (1) cell lysate.](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-7B9A4-Western-Blot-NBP2-52494-img0005.jpg "Western Blot: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Analysis using IL28A mouse mAb against SK-Br-3 (1) cell lysate.")
![Immunohistochemistry: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Analysis of paraffin-embedded esophageal cancer tissues using IL28A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-7B9A4-Immunohistochemistry-NBP2-52494-img0002.jpg)
![Western Blot: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Wnalysis using IL28A mAb against HEK293 (1) and IL28A (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-7B9A4-Western-Blot-NBP2-52494-img0004.jpg)
![Western Blot: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Analysis using IL28A mAb against human IL28A (AA: 1-200) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-7B9A4-Western-Blot-NBP2-52494-img0003.jpg)
![Immunohistochemistry: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Analysis of paraffin-embedded brain tissues using IL28A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-7B9A4-Immunohistochemistry-NBP2-52494-img0001.jpg)
![ELISA: IL-28A/IFN-lambda 2 Antibody (7B9A4) [NBP2-52494] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/IL-28A-IFN-lambda-2-Antibody-7B9A4-ELISA-NBP2-52494-img0006.jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Western-Blot-NB110-96875-img0008.jpg "Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Immunohistochemistry-NB110-96875-img0009.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review. Analysis using the DyLight-550 conjugated antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-fragment-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-74974-img0003.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review. Analysis using the DyLight-550 conjugated antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - MCF7 human breast cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-fragment-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-74974-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-fragment-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-74974-img0002.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-32-BSA-Free-ELISA-NBP1-05182-img0001.jpg "ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.")