Goat anti-Human IgG Fc Secondary Antibody [DyLight 488]

Immunocytochemistry: Goat anti-Human IgG Fc Secondary Antibody [DyLight 488] [NBP1-74980] - SKBR3 human breast cancer cell line. Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: DyLight 488
• Concentration: LYOPH

Goat anti-Human IgG Fc Secondary Antibody [DyLight 488] Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.
• Immunogen: Purified Human IgG, Fc fragment
• Specificity: Based on IEP, this antibody reacts with heavy gamma chains on human IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20 - 1:2000
  • Immunocytochemistry
  • Immunomicroscopy 1:20 - 1:2000

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
• Preservative: 0.05% Sodium Azide
• Concentration: LYOPH
• Purity: Affinity purified
• Reconstitution Instructions: Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NBP1-74980)(1)

Publication using NBP1-74980

• Nagahama K, Isomura A, Shimoyamada H et al Membranous nephropathy with masked polyclonal IgG deposits associated with primary Sjögren's syndrome CEN Case Rep 2020-08-10 [PMID: 32770308]
  
  Details:
  Citation using the DyLight 488 version of this antibody.

Reviews for IgG Fc Antibody (NBP1-74980) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Ben Marcow ICC Human 02/27/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Human breast cancer cell line SKBR3 and this product
• Species: Human
• Lot: 19-126-121311

reviewed by: Sophie Marshall ICC Human 06/28/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: MDA 468 cell lines
• Species: Human
• Lot: 19-126-121311

Bioinformatics

• Uniprot:
  • O75015()
  • O75015()
  • O75015()