Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF974-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP2-44230AFP488 | |||
| Alexa Fluor Plus 555 | NBP2-44230AFP555 | |||
| Alexa Fluor Plus 594 | NBP2-44230AFP594 | |||
| Alexa Fluor Plus 647 | NBP2-44230AFP647 | |||
| Alexa Fluor Plus 680 | NBP2-44230AFP680 | |||
| Biotin | NB600-614-0.1mg | |||
| HRP | NB120-20732-0.1mg | |||
![Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (KA/G & Park, et al., 2006).](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Western-Blot-NBP1-77938-img0004.jpg "Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (KA/G & Park, et al., 2006).")
![Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Immunohistochemistry-NBP1-77938-img0007.jpg)
![Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Analysis of MLF1IP pT78 at the kinetochores of HeLa cells in different phases of the cell cycle. Fluorescent signals were detectable at the kinetochores as early as G2, became most abundant in prophase cells with a discernible nuclear envelope, and gradually diminished as cells proceeded through mitosis (Kang & Park, et al., 2006).](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Immunohistochemistry-NBP1-77938-img0006.jpg)
![N/A General Laboratory Research Reagent [Unconjugated]](http://images.novusbio.com/fullsize2/General_Laboratory_Research_Reagent_DY992_ELISA_2590.jpg "N/A General Laboratory Research Reagent [Unconjugated]")
![Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-Western-Blot-NBP2-26510-img0010.jpg "Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.")
![Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot: Albumin Antibody [Biotin] [NB600-929] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-Western-Blot-NBP2-26510-img0005.jpg)
![SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510]](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-SDS-Page-NBP2-26510-img0007.jpg)
![SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510] - SDS-PAGE of Mouse Mouse Serum Albumin antibody.Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug.Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG.Other band(s): none.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-SDS-Page-NBP2-26510-img0011.jpg)
![ELISA: Mouse Serum Albumin Antibody [NBP2-26510] - ELISA results of purified Mouse Mouse Serum Albumin antibody tested against Mouse Mouse Serum Albumin. Each well was coated in duplicate with 1.0 ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-ELISA-NBP2-26510-img0012.jpg)
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![N/A ELISA Control Group [Unconjugated]](http://images.novusbio.com/fullsize2/General_Laboratory_Research_Reagent_QC011_ELISA_2603.jpg "N/A ELISA Control Group [Unconjugated]")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Immunohistochemistry: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - IHC staining of human frozenskin with 319F6](http://images.novusbio.com/fullsize/CCL20-MIP-3-alpha-Antibody-319F6-06-Immunohistochemistry-DDX0430P-100-img0004.jpg "Immunohistochemistry: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - IHC staining of human frozenskin with 319F6")
![Immunohistochemistry: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - Staining of human tonsil frozen section.](http://images.novusbio.com/fullsize/CCL20-MIP-3-alpha-Antibody-319F6-06-Immunohistochemistry-DDX0430P-100-img0003.jpg)
![ELISA: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - MIP3a production measured by Elisa(319F6/206D9):renal carcinoma cell line (CHA)were activated by increasing doses of IL1(beta).](http://images.novusbio.com/fullsize/CCL20-MIP-3-alpha-Antibody-319F6-06-ELISA-DDX0430P-100-img0006.jpg)
![Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0005.jpg "Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.")
![Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230] - Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).](http://images.novusbio.com/fullsize/Pepsin-Antibody-Immunohistochemistry-Paraffin-NBP2-44230-img0001.jpg)
![Western Blot: Pepsin Antibody [NBP2-44230] - lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0002.jpg)
![Western Blot: Pepsin Antibody [NBP2-44230] - Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0003.jpg)
![N/A RGM-A [HRP]](http://images.novusbio.com/fullsize2/DATA_RGMA_MRGMA0_ELISA_1024.jpg "N/A RGM-A [HRP]")
![N/A RGM-A [HRP]](http://images.novusbio.com/fullsize2/RGM-A_MRGMA0_ELISA_315.jpg)
![N/A RGM-A [HRP]](http://images.novusbio.com/fullsize2/RGM-A_MRGMA0_ELISA_314.jpg)
![Western Blot: Aquaporin-11 Antibody [NBP3-12234] - WB. 1:500 antibody dilution in diluObuffer](http://images.novusbio.com/fullsize/Aquaporin-11-Antibody-Western-Blot-NBP3-12234-img0003.jpg "Western Blot: Aquaporin-11 Antibody [NBP3-12234] - WB. 1:500 antibody dilution in diluObuffer")
![Immunohistochemistry-Paraffin: Aquaporin-11 Antibody [NBP3-12234] - Aquaporin 11. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 40X magnification. FFPE section.](http://images.novusbio.com/fullsize/Aquaporin-11-Antibody-Immunohistochemistry-Paraffin-NBP3-12234-img0004.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-11 Antibody [NBP3-12234] - Aquaporin 11. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section.](http://images.novusbio.com/fullsize/Aquaporin-11-Antibody-Immunohistochemistry-Paraffin-NBP3-12234-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-11 Antibody [NBP3-12234] - Aquaporin 11 1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 40X magnification. FFPE sections.](http://images.novusbio.com/fullsize/Aquaporin-11-Antibody-Immunohistochemistry-Paraffin-NBP3-12234-img0002.jpg)
![Western Blot: HIPK4 Antibody [NBP3-12399] - 1) Wild type mouse testis extract 10 uL. ) 2) kO mouse testis extract 10 uL. 3) 3T3 cells overexpressing mouse 2.5 uL. Apparent MW is 72-80 KDa. 1:250 dilution in DilObuffer.](http://images.novusbio.com/fullsize/HIPK4-Antibody-Western-Blot-NBP3-12399-img0006.jpg "Western Blot: HIPK4 Antibody [NBP3-12399] - 1) Wild type mouse testis extract 10 uL. ) 2) kO mouse testis extract 10 uL. 3) 3T3 cells overexpressing mouse 2.5 uL. Apparent MW is 72-80 KDa. 1:250 dilution in DilObuffer.")
![Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Rat testis. 1:50. IHC blocking buffer. DAB (brown) staining in Hematoxlyin QS (blue) counerstain. 40x magnification.](http://images.novusbio.com/fullsize/HIPK4-Antibody-Immunohistochemistry-Paraffin-NBP3-12399-img0005.jpg)
![Western Blot: HIPK4 Antibody [NBP3-12399] - Lane A: Human over expressing NIH 3T3 cells reduced. Lane B: Mouse over expressing NIH 3T3 cells. Lane C: Mock transfected NIH 3TS3 cells. Dilution 1:500. Total protein per lane 2.5 ug. Apparent MW is 72-80 KDa.](http://images.novusbio.com/fullsize/HIPK4-Antibody-Western-Blot-NBP3-12399-img0001.jpg)
![Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining in Hematoxlyin QS (blue) counerstain. 40x magnification.](http://images.novusbio.com/fullsize/HIPK4-Antibody-Immunohistochemistry-Paraffin-NBP3-12399-img0002.jpg)
![Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Baboon kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining in Hematoxlin QS (blue) counterstain. 40x magnification.](http://images.novusbio.com/fullsize/HIPK4-Antibody-Immunohistochemistry-Paraffin-NBP3-12399-img0003.jpg)