HIPK4 Antibody

Western Blot: HIPK4 Antibody [NBP3-12399] - 1) Wild type mouse testis extract 10 uL. ) 2) kO mouse testis extract 10 uL. 3) 3T3 cells overexpressing mouse 2.5 uL. Apparent MW is 72-80 KDa. 1:250 dilution in DilObuffer.

Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Rat testis. 1:50. IHC blocking buffer. DAB (brown) staining in Hematoxlyin QS (blue) counerstain. 40x magnification.

Western Blot: HIPK4 Antibody [NBP3-12399] - Lane A: Human over expressing NIH 3T3 cells reduced. Lane B: Mouse over expressing NIH 3T3 cells. Lane C: Mock transfected NIH 3TS3 cells. Dilution 1:500. Total protein per lane 2.5 ug. Apparent MW is 72-80 KDa.

Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining in Hematoxlyin QS (blue) counerstain. 40x magnification.

Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Baboon kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining in Hematoxlin QS (blue) counterstain. 40x magnification.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HIPK4 Antibody Summary

• Description: Novus Biologicals Rabbit HIPK4 Antibody (NBP3-12399) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken within amino acid region 470-550 on human Homeodomain-interacting protein kinase 4.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIPK4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:150
  • Immunoprecipitation 1:200
  • Western Blot 1:2500-1:5000

Reactivity Notes

Baboon (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HIPK4 Antibody

• EC 2.7.11.1
• FLJ32818
• homeodomain interacting protein kinase 4
• homeodomain-interacting protein kinase 4

Background

Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter. May act as a corepressor of transcription factors (Potential)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIPK4