Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75398 | |||
| Pre-adsorbed | NBP1-75399 | |||
| Pre-adsorbed | NBP1-75401 | |||
| Pre-adsorbed | NBP1-75402 | |||
| Pre-adsorbed | NBP1-75398-0.5mg | |||
| Pre-adsorbed | NBP1-75398JF549 | |||
| Pre-adsorbed | NBP1-75398JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74860 | |||
| Pre-adsorbed | NBP1-74861 | |||
| Pre-adsorbed | NBP1-74862 | |||
| Pre-adsorbed | NBP1-74864 | |||
| Pre-adsorbed | NBP1-74860-0.5mg | |||
| Pre-adsorbed | NBP1-74860JF549 | |||
| Pre-adsorbed | NBP1-74860JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14183 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB18741-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14063 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14132 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB104741-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10050-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79874 | |||
![Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunocytochemistry-Immunofluorescence-NBP3-13780-img0002.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0004.jpg)
![Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Flow cytometric analysis of PFA-fixed HeLa cells. SP100 antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Flow-Cytometry-NBP3-13780-img0001.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0003.jpg)
![Protein Array: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Antibody (PCRP-SP100-1B9).](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Protein-Array-NBP3-13780-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunocytochemistry-Immunofluorescence-NBP3-13937-img0002.jpg "Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunohistochemistry-Paraffin-NBP3-13937-img0004.jpg)
![Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Flow-Cytometry-NBP3-13937-img0001.jpg)
![Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunohistochemistry-Paraffin-NBP3-13937-img0003.jpg)
![Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HOXB2 Antibody (PCRP-HOXB2-1F2).](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Protein-Array-NBP3-13937-img0006.jpg)
![Western Blot: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Western Blot Analysis of human heart tissue lysate using Phosphoglucomutase 5 antibody (PGM5/3552).](http://images.novusbio.com/fullsize/Phosphoglucomutase-5-Antibody-PGM5-3552-Western-Blot-NBP3-13996-img0001.jpg "Western Blot: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Western Blot Analysis of human heart tissue lysate using Phosphoglucomutase 5 antibody (PGM5/3552).")
![Immunohistochemistry-Paraffin: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Formalin-fixed, paraffin-embedded human uterus stained with Phosphoglucomutase 5 antibody (PGM5/3552)at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Phosphoglucomutase-5-Antibody-PGM5-3552-Immunohistochemistry-Paraffin-NBP3-13996-img0003.jpg)
![Immunohistochemistry-Paraffin: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Formalin-fixed, paraffin-embeddedhuman bladderstained with Phosphoglucomutase 5 antibody (PGM5/3552) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Phosphoglucomutase-5-Antibody-PGM5-3552-Immunohistochemistry-Paraffin-NBP3-13996-img0002.jpg)
![Protein Array: Phosphoglucomutase 5 Antibody (PGM5/3552) [NBP3-13996] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Phosphoglucomutase 5 Antibody (PGM5/3552).](http://images.novusbio.com/fullsize/Phosphoglucomutase-5-Antibody-PGM5-3552-Protein-Array-NBP3-13996-img0005.jpg)
![Western Blot: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Western Blot Analysis of K562 cell lysate using NPM1 Antibody (NPM1/3287).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-3287-Western-Blot-NBP2-79741-img0003.jpg "Western Blot: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Western Blot Analysis of K562 cell lysate using NPM1 Antibody (NPM1/3287).")
![Immunocytochemistry/Immunofluorescence: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Immunofluorescence staining of HeLa cells using NPM1 antibody (NPM1/3287) followed by GAM IgG-CF488 (green). Membrane stained with phalloidin (red).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-3287-Immunocytochemistry-Immunofluorescence-NBP2-79741-img0005.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/3287).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-3287-Immunohistochemistry-Paraffin-NBP2-79741-img0001.jpg)
![Flow Cytometry: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Flow Cytometric Analysis of PFA-fixed HeLa cells using NPM1 antibody (NPM1/3287) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-3287-Flow-Cytometry-NBP2-79741-img0004.jpg)
![Protein Array: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NPM1 Antibody (NPM1/3287) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-3287-Protein-Array-NBP2-79741-img0002.jpg)
![Western Blot: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Western Blot Analysis of A431 cell lysate using NPM1 Antibody (NPM1/1902).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Western-Blot-NBP2-79739-img0004.jpg "Western Blot: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Western Blot Analysis of A431 cell lysate using NPM1 Antibody (NPM1/1902).")
![Immunocytochemistry/Immunofluorescence: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Immunofluorescence staining of HeLa cells using NPM1-Monospecific Mouse Monoclonal Antibody (NPM1/1902) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain.](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Immunocytochemistry-Immunofluorescence-NBP2-79739-img0005.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with NPM1 Antibody (NPM1/1902).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Immunohistochemistry-Paraffin-NBP2-79739-img0002.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/1902).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Immunohistochemistry-Paraffin-NBP2-79739-img0001.jpg)
![Protein Array: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NPM1 Antibody (NPM1/1902) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Protein-Array-NBP2-79739-img0003.jpg)