Human Kappa Light Chain Antibody (999916) [Unconjugated]

Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human kappa light chain protein, human lambda light chain protein (negative control), and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Kappa Light Chain at approximately 32 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Ig Kappa Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA
• Clone: 999916
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Human Kappa Light Chain Antibody (999916) [Unconjugated] Summary

• Immunogen: Human Ig Kappa Light Chain
• Specificity: Detects Human Ig Kappa Light Chain in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Kappa Light Chain Antibody (999916) [Unconjugated]

• ig kappa chain c region;Immunoglobulin K;immunoglobulin kappa light chain;Kappa LC
• Ig Kappa Light Chain

Background

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain.  Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Human Kappa Light Chain Antibody (MAB10050)(1)

Publication using MAB10050

• P Curto, A Barro, C Almeida, RS Vieira-Pir, I Simões The Retropepsin-Type Protease APRc as a Novel Ig-Binding Protein and Moonlighting Immune Evasion Factor of Rickettsia MBio, 2021-12-07;12(6):e0305921. 2021-12-07 [PMID: 34872352] (Western Blot, Human)