Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7441APC | |||
| Biotin | NB7441B | |||
| DyLight 350 | NB7441UV | |||
| DyLight 405 | NB7441V | |||
| DyLight 488 | NB7441G | |||
| DyLight 550 | NB7441R | |||
| DyLight 594 | NB7441DL594 | |||
| DyLight 650 | NB7441C | |||
| DyLight 680 | NB7441FR | |||
| DyLight 755 | NB7441IR | |||
| FITC | NB7443 | |||
| HRP | NB7444 | |||
| Janelia Fluor 525 | NB7441JF525 | |||
| Janelia Fluor 669 | NB7441JF669 | |||
| PE | NB7441PE | |||
| PerCP | NB7441PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB44781U-100UG | |||
| Alexa Fluor 405 | FAB44781V-100UG | |||
| Alexa Fluor 488 | FAB44781G-100UG | |||
| Alexa Fluor 594 | FAB44781T-100UG | |||
| Alexa Fluor 647 | FAB44781R-100UG | |||
| Alexa Fluor 700 | FAB44781N-100UG | |||
| Alexa Fluor 750 | FAB44781S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB44781-SP | |||
| Carrier Free | FAB44781T-100UG | |||
| Carrier Free | FAB44781R-100UG | |||
| Carrier Free | FAB44781G-100UG | |||
| Carrier Free | FAB44781S-100UG | |||
| Carrier Free | FAB44781N-100UG | |||
| Carrier Free | FAB44781V-100UG | |||
| Carrier Free | FAB44781U-100UG | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7176APC | |||
| Biotin | NB7176B | |||
| DyLight 350 | NB7176UV | |||
| DyLight 405 | NB7176V | |||
| DyLight 488 | NB7176G | |||
| DyLight 550 | NB7176R | |||
| DyLight 594 | NB7176DL594 | |||
| DyLight 650 | NB7176C | |||
| DyLight 680 | NB7176FR | |||
| DyLight 755 | NB7176IR | |||
| FITC | NB7178 | |||
| HRP | NB7179 | |||
| Janelia Fluor 525 | NB7176JF525 | |||
| Janelia Fluor 669 | NB7176JF669 | |||
| PE | NB7176PE | |||
| PerCP | NB7176PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-816-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7338APC | |||
| Biotin | NB7338B | |||
| DyLight 350 | NB7338UV | |||
| DyLight 405 | NB7338V | |||
| DyLight 488 | NB7338G | |||
| DyLight 550 | NB7338R | |||
| DyLight 594 | NB7338DL594 | |||
| DyLight 650 | NB7338C | |||
| DyLight 680 | NB7338FR | |||
| DyLight 755 | NB7338IR | |||
| FITC | NB7340 | |||
| HRP | NB7341 | |||
| Janelia Fluor 525 | NB7338JF525 | |||
| Janelia Fluor 669 | NB7338JF669 | |||
| PE | NB7338PE | |||
| PerCP | NB7338PCP | |||
![Western Blot: HLA DQ/DR/DP Antibody (CR3/43) - Azide and BSA Free [NBP2-54507] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP antibody (CR3/43).](http://images.novusbio.com/fullsize/HLA-DPB1-Antibody-CR3-43-Azide-and-BSA-Free-Western-Blot-NBP2-54507-img0005.jpg "Western Blot: HLA DQ/DR/DP Antibody (CR3/43) - Azide and BSA Free [NBP2-54507] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP antibody (CR3/43).")
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (CR3/43) - Azide and BSA Free [NBP2-54507] - Immunofluorescent staining of Raji cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DPB1-Antibody-CR3-43-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54507-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (CR3/43) - Azide and BSA Free [NBP2-54507] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Monoclonal Antibody (CR3/43).](http://images.novusbio.com/fullsize/HLA-DPB1-Antibody-CR3-43-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54507-img0001.jpg)
![Flow Cytometry: HLA DQ/DR/DP Antibody (CR3/43) - Azide and BSA Free [NBP2-54507] - Flow Cytometric Analysis of Human Raji cells using HLA DQ/DR/DP antibody (CR3/43).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/HLA-DPB1-Antibody-CR3-43-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54507-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (CR3/43) - Azide and BSA Free [NBP2-54507] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Monoclonal Antibody (CR3/43).](http://images.novusbio.com/fullsize/HLA-DPB1-Antibody-CR3-43-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54507-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Confocal Immunofluorescence of MCF-7 cells. EpCAM/TROP1 Antibody (EGP40/1384). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54333-img0003.jpg "Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Confocal Immunofluorescence of MCF-7 cells. EpCAM/TROP1 Antibody (EGP40/1384). labeled with CF488 (Green); Red Dot is used to label the nuclei.")
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with EpCAM/TROP1 Antibody (EGP40/1384) - (ECD).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54333-img0004.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Formalin-fixed, paraffin-embedded Human Ovarian Carcinoma stained with EpCAM Monoclonal Antibody (EGP40/1384).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54333-img0001.jpg)
![Protein Array: EpCAM/TROP1 Antibody (EGP40/1384) - (ECD) - Azide and BSA Free [NBP2-54333] - Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM/TROP1 Antibody (EGP40/1384) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1384-ECD-Azide-and-BSA-Free-Protein-Array-NBP2-54333-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0007.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of MCF-7 cells with ErbB2/Her2 Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0004.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0002.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0008.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Protein-Array-NBP2-33064-img0003.jpg)
![Western Blot: ENPP-1 Antibody [NB600-816] - Western blot analysis of ENPP-1 in HepG2 cell lysate at 2.0 ug/ml.](http://images.novusbio.com/fullsize/ENPP-1-Antibody-Western-Blot-NB600-816-img0002.jpg "Western Blot: ENPP-1 Antibody [NB600-816] - Western blot analysis of ENPP-1 in HepG2 cell lysate at 2.0 ug/ml.")
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NB600-816] - ENPP1 Antibody [NB600-816] - ENPP1 antibody was tested in human liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NB600-816-img0003.jpg)
![Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/T7-Epitope-Tag-Antibody-Western-Blot-NB600-371-img0002.jpg "Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.")
![Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/T7-Epitope-Tag-Antibody-Western-Blot-NB600-371-img0001.jpg)