Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ...read more
Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).
Novus Biologicals Goat T7 Epitope Tag Antibody - BSA Free (NB600-371) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-T7 Epitope Tag Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400+ (see reference) *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.
Publications
Read Publications using NB600-371 in the following applications:
Human reactivity reported in scientific literature (PMID: 27226551).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for T7 Epitope Tag Antibody - BSA Free
MASMTGGQQMG epitope tag
MASMTGGQQMG tag
T7 epitope tag
Background
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for T7 Epitope Tag Antibody (NB600-371)(5)
We have publications tested in 1 confirmed species: Human.
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![Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/T7-Epitope-Tag-Antibody-Western-Blot-NB600-371-img0002.jpg "Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.")
![Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/T7-Epitope-Tag-Antibody-Western-Blot-NB600-371-img0001.jpg "Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
