T7 Epitope Tag Antibody

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Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ...read more
Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Product Details

Summary
Reactivity Hu, All-NASpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

T7 Epitope Tag Antibody Summary

Immunogen
Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA Primary-1:1000-1:30000; Coating: 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunoprecipitation
Application Notes
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400+ (see reference) *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.
Publications
Read Publications using
NB600-371 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27226551).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for T7 Epitope Tag Antibody

  • MASMTGGQQMG epitope tag
  • MASMTGGQQMG tag
  • T7 epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T7 Epitope Tag Antibody (NB600-371)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose NB600-378
Biotin NB600-371B
FITC NB600-376
HRP NB600-377

Additional T7 Epitope Tag Products

Array NB600-371

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