T7 Epitope Tag Antibody

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Western Blot: T7 Epitope Tag Antibody [NB600-371] - Detection of T7-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity ...read more
Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).
Western Blot: T7 Epitope Tag Antibody [NB600-371] - Analysis using the Biotin conjugate of NB600-371. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at ...read more
Western Blot: T7 Epitope Tag Antibody [NB600-371] - Analysis using the HRP conjugate of NB600-371. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ...read more

Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

T7 Epitope Tag Antibody Summary

Immunogen
Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA Primary-1:1000-1:30000; Coating: 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
Application Notes
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400+ (see reference) *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.
Publications
Read Publications using
NB600-371 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27226551).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for T7 Epitope Tag Antibody

  • MASMTGGQQMG epitope tag
  • MASMTGGQQMG tag
  • T7 epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T7 Epitope Tag Antibody (NB600-371)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Human
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB600-371 Applications Species
Mashiko T, Sakashita E, Kasashima K et al. Developmentally-regulated RNA-binding Protein 1 (Drb1)/RNA-binding Motif Protein 45 (RBM45), a Nuclear-cytoplasmic Trafficking Protein, Forms TAR DNA-binding Protein 43 (TDP-43)-mediated Cytoplasmic Aggregates. J. Biol. Chem. May 12 2016 [PMID: 27226551] (WB, Human) WB Human
Swartz JE, Bor YC, Misawa Y et al. The shuttling SR protein 9G8 plays a role in translation of unspliced mRNA containing a constitutive transport element J Biol Chem 2007 Jul [PMID: 17513303]

Details:
This citation used the Agarose Immobilized version of this antibody.
Caracino D, Jones C, Compton M et al. The N-terminus of Dictyostelium Scar interacts with Abi and HSPC300 and is essential for proper regulation and function Mol Biol Cell 2016 Nov 7 [PMID: 17314411]

Details:
This citation used the Agarose Immobilized version of this antibody.
Glater EE, Megeath LJ, Stowers RS et al. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent. J Cell Biol 2006 May [PMID: 16717129]
Jacob, C et al. C-CBL Mediates Ubiquitination, degradation Down-Regulation of Human Protease-Activated Receptor 2 (PAR2). JBC Papers in Press. Manuscript M500109200. 2005 Feb 11. [PMID: 15708858]
Caracino D, Jones C, Compton M et al. The N-terminus of Dictyostelium Scar interacts with Abi and HSPC300 and is essential for proper regulation and function Mol Biol Cell

Details:
This publication used the Agarose Immobilized conjugated form of this antibody (Cat# NB600-378).
Swartz JE, Bor YC, Misawa Y et al. The shuttling SR protein 9G8 plays a role in translation of unspliced mRNA containing a constitutive transport element J Biol Chem

Details:
This publication used the Agarose Immobilized conjugated form of this antibody (Cat# NB600-378).

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose Immobilized Labeled NB600-378
Biotin Labeled NB600-371B
FITC Labeled NB600-376
HRP Labeled NB600-377

Additional T7 Epitope Tag Products

T7 Epitope Tag NB600-371

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