T7 Epitope Tag Antibody [Biotin]

Images

 
Western Blot: T7 Epitope Tag Antibody [Biotin] [NB600-371B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP ...read more

Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

T7 Epitope Tag Antibody [Biotin] Summary

Immunogen
Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:100000
  • ELISA 1:100-1:2000
Readout System

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27226551).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and 0.2% BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for T7 Epitope Tag Antibody [Biotin]

  • MASMTGGQQMG epitope tag
  • MASMTGGQQMG tag
  • T7 epitope tag

Background

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T7 Epitope Tag Antibody (NB600-371B) (0)

There are no publications for T7 Epitope Tag Antibody (NB600-371B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for T7 Epitope Tag Antibody (NB600-371B) (0)

There are no reviews for T7 Epitope Tag Antibody (NB600-371B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for T7 Epitope Tag Antibody (NB600-371B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose Labeled NB600-378
FITC Labeled NB600-376
HRP Labeled NB600-377

Additional Array Products

Array NB600-371B

Research Areas for T7 Epitope Tag Antibody (NB600-371B)

Find related products by research area.

Blogs on T7 Epitope Tag

There are no specific blogs for T7 Epitope Tag, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our T7 Epitope Tag Antibody [Biotin] and receive a gift card or discount.