Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51664SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23891SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2548-SP | |||
| Carrier Free | IC2548R-100UG | |||
| Carrier Free | IC2548U-100UG | |||
| Carrier Free | IC2548V-100UG | |||
| Carrier Free | IC2548S-100UG | |||
| Carrier Free | IC2548T-100UG | |||
| Carrier Free | IC2548N-100UG | |||
| Carrier Free | IC2548G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56048SS | |||
![N/A Leptin R [Biotin]](http://images.novusbio.com/fullsize2/DATA_Leptin_R_DY389_ELISA_1987.jpg "N/A Leptin R [Biotin]")
![N/A CD44 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CD44_DY704505_ELISA_2500.jpg "N/A CD44 [Biotin]")
![N/A CXCL8/IL-8 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL8_DY1608_ELISA_1685.jpg "N/A CXCL8/IL-8 [Biotin]")
![Western Blot: OCT4 Antibody (7E7) [NBP1-51664] - Analysis using OCT4 mouse mAb against NTERA-2 cell lysate.](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Western-Blot-NBP1-51664-img0007.jpg "Western Blot: OCT4 Antibody (7E7) [NBP1-51664] - Analysis using OCT4 mouse mAb against NTERA-2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664] - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Immunocytochemistry-Immunofluorescence-NBP1-51664-img0008.jpg)
![Flow Cytometry: OCT4 Antibody (7E7) [NBP1-51664] - Flow cytometric analysis of Jurket cells using OCT4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Flow-Cytometry-NBP1-51664-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664] - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Immunocytochemistry-Immunofluorescence-NBP1-51664-img0006.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-23891-img0002.jpg "Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.")
![Flow Cytometry: Dicer Antibody [NBP2-23891] - Analysis of HeLa cells transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using this antibody. Purple represents isotype control staining.](http://images.novusbio.com/fullsize/Dicer-Antibody-Flow-Cytometry-NBP2-23891-img0003.jpg)
![ELISA: Dicer Antibody [NBP2-23891] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-ELISA-NBP2-23891-img0001.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Western-Blot-NBP2-20866-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vitronectin stained by Vitronectin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0008.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Human colon carcinoma, using Vitronectin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0003.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (NBP2-20866) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20866_rabbit-polyclonal-vitronectin-antibody-910202420104489.jpg)
![N/A Uteroglobin/SCGB1A1 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Uteroglobin_DY4218_ELISA_2018.jpg "N/A Uteroglobin/SCGB1A1 [Biotin]")
![N/A TWEAK/TNFSF12 [Biotin]](http://images.novusbio.com/fullsize2/DATA_TWEAK_DY1237_ELISA_1609.jpg "N/A TWEAK/TNFSF12 [Biotin]")
![Western Blot: Sars Spike Protein Antibody [NB100-56048] - Used at 1 ug/ml to analyze SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.](http://images.novusbio.com/fullsize/Sars-Spike-Protein-Antibody-Western-Blot-NB100-56048-img0002.jpg "Western Blot: Sars Spike Protein Antibody [NB100-56048] - Used at 1 ug/ml to analyze SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.")
![N/A IL-1 beta/IL-1F2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL1_beta_DY3340_ELISA_1937.jpg "N/A IL-1 beta/IL-1F2 [Biotin]")