SARS Spike Protein Antibody - BSA Free

Western Blot: Sars Spike Protein Antibody [NB100-56048] - Used at 1 ug/ml to analyze SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.

• Reactivity: V
• Applications: WB, ELISA, Func
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SARS Spike Protein Antibody - BSA Free Summary

• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 288-303 (AVDCALDPLSETKCTL) from the S (Spike glycoprotein)(Spike protein 1) for the Human SARS coronavirus (Genbank accession no. YP_009724390)
• Specificity: The antibody was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.1-1 ug/ml
  • In vitro assay reported in scientific literature (PMID 34120764)
  • Western Blot 0.1-1 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (69%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SARS Spike Protein Antibody - BSA Free

• E2 glycoprotein
• E2
• Human coronavirus spike glycoprotein
• Peplomer protein
• S glycoprotein
• S
• SARS coronavirus S protein
• SARS coronavirus Spike Protein
• SARS CoV S glycoprotein
• SARS CoV Spike protein
• SARS CoV
• SARS-CoV S protein
• SARS-COV Spike
• SARSCoV
• Severe acute respiratory syndrome spike glycoprotein
• Severe acute respiratory syndrome virus spike glycoprotein
• Spike glycoprotein
• VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS Spike Protein Antibody (NB100-56048)(4)

Publications using NB100-56048

• Dey A, Chozhavel Rajanathan TM, Chandra H et al. Immunogenic potential of DNA vaccine candidate, ZyCoV-D against SARS-CoV-2 in animal models Vaccine 2021-07-05 [PMID: 34120764] (In vitro)
• Liniger M, Zuniga A, Tamin A et al. Induction of neutralising antibodies and cellular immune responses against SARS coronavirus by recombinant measles viruses. Vaccine. 2008-04-16 [PMID: 18346823] (WB)
• Wang Wei, Ye Linbai, Ye Li et al. Up-regulation of IL-6 and TNF-alpha induced by SARS-coronavirus spike protein in murine macrophages via NF-kappaB pathway. Virus Res. 2007-09-01 [PMID: 17532082]
• Xiao X, Chakraborti S, Dimitrov AS et al. The SARS-CoV S glycoprotein: expression and functional characterization. Biochem Biophys Res Commun. 2003-12-26 [PMID: 14651994]

FAQs for SARS Spike Protein Antibody (NB100-56048). (Showing 1 - 2 of 2 FAQs).

1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
   • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
   • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.