Sars Spike Protein Antibody

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Western Blot: Sars Spike Protein Antibody [NB100-56048] - Analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using NB100-56048 at 0.1 ug/ml.
Western Blot: Sars Spike Protein Antibody [NB100-56048] - analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody. 1 ug/ml.

Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Sars Spike Protein Antibody Summary

Immunogen
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 288-303 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851).
Specificity
The was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68 kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-1 ug/ml
  • ELISA 0.1-1 ug/ml
Publications
Read Publications using NB100-56048.

Reactivity Notes

Spike glycoprotein of the human SARS coronavirus.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Sars Spike Protein Antibody

  • E2 glycoprotein
  • E2
  • Human coronavirus spike glycoprotein
  • Peplomer protein
  • S glycoprotein
  • S
  • Severe acute respiratory syndrome spike glycoprotein
  • Severe acute respiratory syndrome virus spike glycoprotein
  • Spike glycoprotein
  • VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Sars Spike Protein Antibody (NB100-56048)(3)

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Video Protocols

WB Video Protocol

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