Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1341V | |||
| FITC | NB21-1341F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1341SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7222APC | |||
| Biotin | NB7222B | |||
| DyLight 350 | NB7222UV | |||
| DyLight 405 | NB7222V | |||
| DyLight 488 | NB7222G | |||
| DyLight 550 | NB7222R | |||
| DyLight 594 | NB7222DL594 | |||
| DyLight 650 | NB7222C | |||
| DyLight 680 | NB7222FR | |||
| DyLight 755 | NB7222IR | |||
| FITC | NB7223 | |||
| HRP | NB7224 | |||
| PE | NB7222PE | |||
| PerCP | NB7222PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26221-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25226 | |||
| BSA Free | NBP2-25223SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-454SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47471SS | |||
| Azide and BSA Free | NBP2-80576 | |||
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![N/A CCL28 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CCL28_DY717_ELISA_2192.jpg "N/A CCL28 [Biotin]")
![N/A IL-10 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL10_DY736_ELISA_2204.jpg "N/A IL-10 [Biotin]")
![Western Blot: Histone H3 [Monomethyl Lys56] Antibody [NB21-1341] - Antibody was tested in NIH-3T3 histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys56]-Antibody-Western-Blot-NB21-1341-img0007.jpg "Western Blot: Histone H3 [Monomethyl Lys56] Antibody [NB21-1341] - Antibody was tested in NIH-3T3 histone prep lysate. Observed molecular weight is ~15 kDa.")
![Dot Blot: Histone H3 [Monomethyl Lys56] Antibody [NB21-1341] - H3 K56me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys56]-Antibody-Dot-Blot-NB21-1341-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys56] Antibody [NB21-1341] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys56]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1341-img0015.jpg)
![N/A IGF-II/IGF2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IGFII_DY29205_ELISA_2416.jpg "N/A IGF-II/IGF2 [Biotin]")
![Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011413.jpg "Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Flow Cytometry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Overlay Peak curve showing MCF7 cells stained with NBP3-26221 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101142.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of Hela cell with NBP3-26221 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101149.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011417.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of HepG2 cell with NBP3-26221 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011412.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101145.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-2612025101144.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26221_rabbit-hnrnp-c1-c2-mab-9g1-26120251011415.jpg)
![Western Blot: CD39/ENTPD1 Antibody (17B5F11) [NBP2-25223] - Analysis of human (A), mouse (B), and rat brain (C) and a recombinant protein fragment (D) using CD39 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-17B5F11-Western-Blot-NBP2-25223-img0003.jpg "Western Blot: CD39/ENTPD1 Antibody (17B5F11) [NBP2-25223] - Analysis of human (A), mouse (B), and rat brain (C) and a recombinant protein fragment (D) using CD39 antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (17B5F11) [NBP2-25223] - Normal human spleen tissue stained with CD39 antibody (5 ug/mL), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-17B5F11-Immunohistochemistry-Paraffin-NBP2-25223-img0004.jpg)
![Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.](http://images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Western-Blot-NBP1-47546-img0002.jpg "Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).](http://images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Immunocytochemistry-Immunofluorescence-NBP1-47546-img0003.jpg)
![Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Western-Blot-NB100-454-img0003.jpg "Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.")
![Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454] - VG5Q was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human VG5Q polyclonal Antibody (Catalog # NB100-454) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.](http://images.novusbio.com/fullsize/VG5Q-Antibody-Immunohistochemistry-Paraffin-NB100-454-img0004.jpg)
![Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates.](http://images.novusbio.com/fullsize/Androgen-Receptor-Antibody-2H8-Western-Blot-NBP1-47471-img0010.jpg "Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates.")
![Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis of paraffin-embedded human Prostate tissues using Androgen Receptor mouse mAb](http://images.novusbio.com/fullsize/Androgen-Receptor-Antibody-2H8-Immunohistochemistry-Paraffin-NBP1-47471-img0011.jpg)
![Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against Androgen Receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/Androgen-Receptor-Antibody-2H8-Western-Blot-NBP1-47471-img0008.jpg)
![Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against human recombinant Androgen Receptor.](http://images.novusbio.com/fullsize/Androgen-Receptor-Antibody-2H8-Western-Blot-NBP1-47471-img0009.jpg)
![Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis of paraffin-embedded human testis (left) and prostate cancer tissues (right) using Androgen Receptor mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Androgen-Receptor-Antibody-2H8-Immunohistochemistry-Paraffin-NBP1-47471-img0012.jpg)