Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41572SS | |||
| Azide and BSA Free | NBP2-80672 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB648-SP | |||
| Carrier Free | MAB648-500 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-920AFP488 | |||
| Alexa Fluor Plus 555 | NB600-920AFP555 | |||
| Alexa Fluor Plus 594 | NB600-920AFP594 | |||
| Alexa Fluor Plus 647 | NB600-920AFP647 | |||
| Alexa Fluor Plus 680 | NB600-920AFP680 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7281APC | |||
| Biotin | NB7281B | |||
| DyLight 350 | NB7281UV | |||
| DyLight 405 | NB7281V | |||
| DyLight 488 | NB7281G | |||
| DyLight 550 | NB7281R | |||
| DyLight 594 | NB7281DL594 | |||
| DyLight 650 | NB7281C | |||
| DyLight 680 | NB7281FR | |||
| DyLight 755 | NB7281IR | |||
| FITC | NB7283 | |||
| HRP | NB7284-1.0mg | |||
| Janelia Fluor 525 | NB7281JF525 | |||
| Janelia Fluor 669 | NB7281JF669 | |||
| PE | NB7281PE | |||
| PerCP | NB7281PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7100APC | |||
| Biotin | NB7100B | |||
| DyLight 350 | NB7100UV | |||
| DyLight 405 | NB7100V | |||
| DyLight 488 | NB7100G | |||
| DyLight 550 | NB7100R | |||
| DyLight 594 | NB7100DL594 | |||
| DyLight 650 | NB7100C | |||
| DyLight 680 | NB7100FR | |||
| DyLight 755 | NB7100IR | |||
| FITC | NB7102 | |||
| HRP | NB7103 | |||
| Janelia Fluor 525 | NB7100JF525 | |||
| Janelia Fluor 669 | NB7100JF669 | |||
| PE | NB7100PE | |||
| PerCP | NB7100PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54457-100ug | |||
![N/A IL-9 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL9_DY20905_ELISA_2385.jpg "N/A IL-9 [Biotin]")
![Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.](http://images.novusbio.com/fullsize/CITED4-Antibody-HT13-2D6-3-Western-Blot-NB110-41572-img0003.jpg "Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.")
![Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - CITED4 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CITED4 (HT13-2D6.3) Monoclonal Antibody (Catalog # NB110-41572) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cardiomyocytes.](http://images.novusbio.com/fullsize/CITED4-Antibody-HT13-2D6-3-Immunohistochemistry-Paraffin-NB110-41572-img0009.jpg)
![Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CITED4 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/CITED4-Antibody-HT13-2D6-3-Flow-Cytometry-NB110-41572-img0008.jpg)
![N/A CD40/TNFRSF5 [HRP]](http://images.novusbio.com/fullsize2/DATA_CD40_DCCD40_ELISA_618.jpg "N/A CD40/TNFRSF5 [HRP]")
![N/A CD40/TNFRSF5 [HRP]](http://images.novusbio.com/fullsize2/CD40_DCCD40_ELISA_56.jpg)
![Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NB600-920-img0004.jpg "Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.")
![N/A Fibronectin [Biotin]](http://images.novusbio.com/fullsize2/DATA_Fibronectin_DY191805_ELISA_2377.jpg "N/A Fibronectin [Biotin]")
![N/A IL-12/IL-23 p40 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL12_p40_DY1969_ELISA_1741.jpg "N/A IL-12/IL-23 p40 [Biotin]")
![N/A Fas Ligand/TNFSF6 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Fas_Ligand_DY126_ELISA_1615.jpg "N/A Fas Ligand/TNFSF6 [Biotin]")
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0006.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).")
![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Confocal immunofluorescence image of HeLa cells using Galectin-1 Antibody (GAL1/1831). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Immunocytochemistry-Immunofluorescence-NBP2-53212-img0003.jpg)
![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Immunohistochemistry-Paraffin-NBP2-53212-img0001.jpg)
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot of HeLa, A431, K562 and 293 Cell Lysates using Galectin-1 Monoclonal Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0002.jpg)
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of human HeLa cell lysate using Galectin-1 Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0005.jpg)
![Protein Array: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Antibody (GAL1/1831). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Protein-Array-NBP2-53212-img0004.jpg)
![N/A IL-17A/F Heterodimer [HRP]](http://images.novusbio.com/fullsize2/DATA_IL17A_F_Heterodimer_M17AF0_ELISA_925.jpg "N/A IL-17A/F Heterodimer [HRP]")
![N/A IL-17A/F Heterodimer [HRP]](http://images.novusbio.com/fullsize2/IL-17A_F_Heterodimer_M17AF0_ELISA_228.jpg)