Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7281APC | |||
| Biotin | NB7281B | |||
| DyLight 350 | NB7281UV | |||
| DyLight 405 | NB7281V | |||
| DyLight 488 | NB7281G | |||
| DyLight 550 | NB7281R | |||
| DyLight 594 | NB7281DL594 | |||
| DyLight 650 | NB7281C | |||
| DyLight 680 | NB7281FR | |||
| DyLight 755 | NB7281IR | |||
| FITC | NB7283 | |||
| HRP | NB7284-1.0mg | |||
| Janelia Fluor 525 | NB7281JF525 | |||
| Janelia Fluor 669 | NB7281JF669 | |||
| PE | NB7281PE | |||
| PerCP | NB7281PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-920AFP488 | |||
| Alexa Fluor Plus 555 | NB600-920AFP555 | |||
| Alexa Fluor Plus 594 | NB600-920AFP594 | |||
| Alexa Fluor Plus 647 | NB600-920AFP647 | |||
| Alexa Fluor Plus 680 | NB600-920AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41572SS | |||
| Azide and BSA Free | NBP2-80672 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51582SS | |||
| Azide and BSA Free | NBP2-80720 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7100APC | |||
| Biotin | NB7100B | |||
| DyLight 350 | NB7100UV | |||
| DyLight 405 | NB7100V | |||
| DyLight 488 | NB7100G | |||
| DyLight 550 | NB7100R | |||
| DyLight 594 | NB7100DL594 | |||
| DyLight 650 | NB7100C | |||
| DyLight 680 | NB7100FR | |||
| DyLight 755 | NB7100IR | |||
| FITC | NB7102 | |||
| HRP | NB7103 | |||
| Janelia Fluor 525 | NB7100JF525 | |||
| Janelia Fluor 669 | NB7100JF669 | |||
| PE | NB7100PE | |||
| PerCP | NB7100PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7487APC | |||
| Biotin | NB7487B | |||
| DyLight 350 | NB7487UV | |||
| DyLight 405 | NB7487V | |||
| DyLight 488 | NB7487G | |||
| DyLight 550 | NB7487R | |||
| DyLight 594 | NB7487DL594 | |||
| DyLight 650 | NB7487C | |||
| DyLight 680 | NB7487FR | |||
| DyLight 755 | NB7487IR | |||
| FITC | NB7488 | |||
| HRP | NB7489 | |||
| PE | NB7487PE | |||
| PerCP | NB7487PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7488 | |||
| Pre-adsorbed | NB7489 | |||
| Pre-adsorbed | NB7487B | |||
![Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NB600-920-img0004.jpg "Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.")
![Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.](http://images.novusbio.com/fullsize/CITED4-Antibody-HT13-2D6-3-Western-Blot-NB110-41572-img0003.jpg "Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.")
![Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - CITED4 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CITED4 (HT13-2D6.3) Monoclonal Antibody (Catalog # NB110-41572) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cardiomyocytes.](http://images.novusbio.com/fullsize/CITED4-Antibody-HT13-2D6-3-Immunohistochemistry-Paraffin-NB110-41572-img0009.jpg)
![Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CITED4 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/CITED4-Antibody-HT13-2D6-3-Flow-Cytometry-NB110-41572-img0008.jpg)
![Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Western-Blot-NBP1-51582-img0003.jpg "Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0004.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0001.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0002.jpg)
![N/A 4-1BB/TNFRSF9/CD137 [Biotin]](http://images.novusbio.com/fullsize2/DATA_41BB_DY838_ELISA_2242.jpg "N/A 4-1BB/TNFRSF9/CD137 [Biotin]")
![N/A CCL12/MCP-5 [HRP]](http://images.novusbio.com/fullsize2/DATA_CCL12_MCC120_ELISA_949.jpg "N/A CCL12/MCP-5 [HRP]")
![N/A CCL12/MCP-5 [HRP]](http://images.novusbio.com/fullsize2/CCL12_MCC120_ELISA_246.jpg)
![N/A Serpin E1/PAI-1 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Serpin_E1_DY382805_ELISA_2442.jpg "N/A Serpin E1/PAI-1 [Biotin]")
![N/A Insulin [Biotin]](http://images.novusbio.com/fullsize2/DATA_Insulin_DY805605_ELISA_2522.jpg "N/A Insulin [Biotin]")
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![N/A Reg3A [Biotin]](http://images.novusbio.com/fullsize2/DATA_Reg3A_DY594005_ELISA_2483.jpg "N/A Reg3A [Biotin]")