Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed)

Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed). Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: FITC
• Format: Pre-adsorbed
• Concentration: 1.0 mg/ml

Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) Summary

• Immunogen: This Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) was developed against human IgG.
• Specificity: By immunoelectrophoresis and ELISA this Goat anti-Human IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using rhesus and cynomolgus monkey immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to monkey IgG was detected.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7488)(1)

Publication using NB7488

• Santostefano MJ, Kirchner J, Vissinga C et al. Off-target platelet activation in macaques unique to a therapeutic monoclonal antibody. Toxicol Pathol. 2012-08-01 [PMID: 22552394]
  
  Details:
  This citation used the FITC form of this antibody.

Review for IgG (H+L) Antibody (NB7488) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 10/31/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Human cell line
• Species: Human
• Lot: F17