HAF007). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Yes Monoclonal Antibody (Catalog # MAB32051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB32051-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3205-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85369-25ul | |||
CTS002) and counterstained with hematoxylin (blue). View our protocol for ![Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-31297-img0010.jpg "Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: Yes Antibody [NBP1-31297] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Yes-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31297-img0006.jpg)
![Western Blot: Yes Antibody [NBP1-31297] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-31297-img0008.jpg)
![Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-31297-img0009.jpg)
![Western Blot: Yes Antibody [NBP1-85369] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-85369-img0009.jpg "Western Blot: Yes Antibody [NBP1-85369] - Analysis in human cell line U-2 OS.")
![Immunocytochemistry/Immunofluorescence: Yes Antibody [NBP1-85369] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Yes-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85369-img0011.jpg)
![Immunohistochemistry-Paraffin: Yes Antibody [NBP1-85369] - Staining of human kidney shows moderate cytoplasmic positivity in tubule and glomerular cells.](http://images.novusbio.com/fullsize/Yes-Antibody-Immunohistochemistry-Paraffin-NBP1-85369-img0008.jpg)
![Western Blot: Yes Antibody (2D5) [H00007525-M03] - Analysis of YES1 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/Yes-Antibody-2D5-Western-Blot-H00007525-M03-img0005.jpg "Western Blot: Yes Antibody (2D5) [H00007525-M03] - Analysis of YES1 expression in A-431 (Cat # L015V1).")
![Immunohistochemistry-Paraffin: Yes Antibody (2D5) [H00007525-M03] - Analysis of monoclonal antibody to YES1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Yes-Antibody-2D5-Immunohistochemistry-Paraffin-H00007525-M03-img0003.jpg)
![Sandwich ELISA: Yes Antibody (2D5) [H00007525-M03] - Detection limit for recombinant GST tagged YES1 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Yes-Antibody-2D5-Sandwich-ELISA-H00007525-M03-img0004.jpg)
![Western Blot: Yes Antibody (JE37-26) [NBP3-33107] - Western blot analysis of Yes on different lysates with Rabbit anti-Yes antibody (NBP3-33107) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33107) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33107_rabbit-yes-mab-je37-26-28820241419155.jpg "Western Blot: Yes Antibody (JE37-26) [NBP3-33107] - Western blot analysis of Yes on different lysates with Rabbit anti-Yes antibody (NBP3-33107) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33107) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Yes Antibody (JE37-26) [NBP3-33107] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Yes antibody (NBP3-33107) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33107) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33107_rabbit-yes-mab-je37-26-298202414304176.jpg)
![Western Blot: Yes Antibody (3C6) [H00007525-M02] - YES1 monoclonal antibody (M02), clone 3C6. Analysis of YES1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Yes-Antibody-3C6-Western-Blot-H00007525-M02-img0004.jpg "Western Blot: Yes Antibody (3C6) [H00007525-M02] - YES1 monoclonal antibody (M02), clone 3C6. Analysis of YES1 expression in Hela S3 NE.")
![Western Blot: Yes Antibody (3C6) [H00007525-M02] - YES1 monoclonal antibody (M02), clone 3C6 Analysis of YES1 expression in Jurkat.](http://images.novusbio.com/fullsize/Yes-Antibody-3C6-Western-Blot-H00007525-M02-img0005.jpg)
![Proximity Ligation Assay: Yes Antibody (3C6) [H00007525-M02] - Analysis of protein-protein interactions between PTK2B and YES1. Huh7 cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-YES1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Yes-Antibody-3C6-Proximity-Ligation-Assay-H00007525-M02-img0003.jpg)
![Western Blot: Yes Antibody (2A12) [H00007525-M05] - Analysis of YES1 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/Yes-Antibody-2A12-Western-Blot-H00007525-M05-img0004.jpg "Western Blot: Yes Antibody (2A12) [H00007525-M05] - Analysis of YES1 expression in A-431 (Cat # L015V1).")
![Sandwich ELISA: Yes Antibody (2A12) [H00007525-M05] - Detection limit for recombinant GST tagged YES1 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Yes-Antibody-2A12-Sandwich-ELISA-H00007525-M05-img0003.jpg)
![Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 over-expressed 293 cell line, cotransfected with YES1 Validated Chimera RNAi ( Cat # H00007525-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with YES1 monoclonal antibody (M01) clone 2F4 (Cat # H00007525-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Yes-Antibody-2F4-Western-Blot-H00007525-M01-img0003.jpg "Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 over-expressed 293 cell line, cotransfected with YES1 Validated Chimera RNAi ( Cat # H00007525-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with YES1 monoclonal antibody (M01) clone 2F4 (Cat # H00007525-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 expression in transfected 293T cell line by YES1 monoclonal antibody (M01), clone 2F4.Lane 1: YES1 transfected lysate(59.84 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Yes-Antibody-2F4-Western-Blot-H00007525-M01-img0004.jpg)
![Western Blot: Yes1 Overexpression Lysate (Adult Normal) [NBL1-17928] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Yes1.](http://images.novusbio.com/fullsize/Yes1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17928-img0002.jpg "Western Blot: Yes1 Overexpression Lysate (Adult Normal) [NBL1-17928] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Yes1.")
![Western Blot: Yes Antibody (S07-8B3) [NBP3-19980] - Western blot detection of Yes in K562 cell lysates using NBP3-19980 (1:1000 diluted). Predicted band size: 61kDa. Observed band size: 61kDa.](http://images.novusbio.com/fullsize/Yes-Antibody-S07-8B3-Western-Blot-NBP3-19980-img0001.jpg "Western Blot: Yes Antibody (S07-8B3) [NBP3-19980] - Western blot detection of Yes in K562 cell lysates using NBP3-19980 (1:1000 diluted). Predicted band size: 61kDa. Observed band size: 61kDa.")
