| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86030-25ul | |||
HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3716-SP | |||
G-105-C) for 2 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-PLC- gamma2 (Y759) at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes or 10 µg/mL Goat Anti-Human IgM µ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-PLC- gamma2 (Y759) at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7377-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52535-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52534-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB37161-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87558-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22245-50ul | |||
![Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-288202412461651.jpg "Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32827) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-298202414333622.jpg)
![Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunocytochemistry analysis of Raji cells labeling PLC-gamma 2 with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-29202410334047.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Flow cytometric analysis of Raji cells labeling PLC-gamma 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32827, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-392024884627.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Analysis in human lymph node and pancreas tissues. Corresponding PLC-gamma 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Analysis in human lymph node and pancreas tissues. Corresponding PLC-gamma 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0005.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-86030] - Staining of human duodenum shows strong cytoplasmic positivity in lymphoid cells and immunoreactivity in endocrine cells.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86030-img0009.jpg)
NL007) and counterstained with DAPI (blue). View our protocol for 
NL007) and counterstained with DAPI (blue). View our protocol for ![Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Western-Blot-NBP2-52535-img0002.jpg "Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Immunohistochemical analysis of paraffin-embedded Hela tissues using PLC-gamma 2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Immunohistochemistry-Paraffin-NBP2-52535-img0004.jpg)
![Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against human PLCG2 (AA: 826-985) recombinant protein. (Expected MW is 44.6 kDa)](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Western-Blot-NBP2-52535-img0001.jpg)
![ELISA: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-ELISA-NBP2-52535-img0003.jpg)
![Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Western-Blot-NBP2-52534-img0005.jpg "Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Immunofluorescence-NBP2-52534-img0002.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Flow-Cytometry-NBP2-52534-img0001.jpg)
![ELISA: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-ELISA-NBP2-52534-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody [NBP1-87558] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87558-img0005.jpg "Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody [NBP1-87558] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.")
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-87558] - Staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87558-img0002.jpg)
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-33036] - Paraffin-embedded Gastric carcinoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33036-img0006.jpg "Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-33036] - Paraffin-embedded Gastric carcinoma, using antibody at 1:500 dilution.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PLC-gamma 2 Antibody [NBP1-33036] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33036_rabbit-polyclonal-plc-gamma-2-antibody-1610202419405717.jpg)
![Western Blot: PLC-gamma 2 Antibody (SR1168) [NBP3-22245] - Western blot analysis using NBP3-22245 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-22245_rabbit-plc-gamma-2-mab-sr1168-57202317161832.jpg "Western Blot: PLC-gamma 2 Antibody (SR1168) [NBP3-22245] - Western blot analysis using NBP3-22245 in Ramos cell lysate.")
![Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr1217) antibody (NBP3-13270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLC-gamma-2-[p-Tyr1217]-Antibody-Western-Blot-NBP3-13270-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr1217) antibody (NBP3-13270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270] - Untreated (-) and treated (+) Raji whole cell extract (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr1217) antibody (NBP3-13270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLC-gamma-2-[p-Tyr1217]-Antibody-Western-Blot-NBP3-13270-img0001.jpg)
![Western Blot: PLC-gamma 2 [p Tyr759] Antibody [NBP3-13241] - Untreated (-) and treated (+) Raji whole cell extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr759) antibody (NBP3-13241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLC-gamma-2-[p-Tyr759]-Antibody-Western-Blot-NBP3-13241-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLC-gamma 2 [p Tyr759] Antibody [NBP3-13241] - Untreated (-) and treated (+) Raji whole cell extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr759) antibody (NBP3-13241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
