HAF109). Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

2526-IL) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-27 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526). The ND₅₀ is typically 0.200-2.40 µg/mL." class="big_lightbox"> 2526-IL) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-27 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526). The ND₅₀ is typically 0.200-2.40 µg/mL." class="big_thumb" />

2526-IL) was serially diluted and captured by Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2526-IL) was serially diluted and captured by Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2526-IL) was serially diluted and captured by Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2526-IL) was serially diluted and captured by Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB003, blue open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB003, blue open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Human PBMC, treated with 5 µg/mL PHA and 5 ng/mL rhIL-2 (dark orange filled histogram), or untreated (open histogram, yellow line) were stained with Mouse Anti-Human IL-27 Monoclonal Antibody (Catalog # MAB25261) or isotype control antibody (Catalog # MAB003, blue open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

1479-TC) is immobilized at 0.500 μg/mL (100 μL/well), Recombinant Human IL‑27 His-tag Biotinylated Protein (Catalog # BT2526) binds with an ED₅₀ of 4.00‑40.0 ng/mL." class="big_lightbox"> 1479-TC) is immobilized at 0.500 μg/mL (100 μL/well), Recombinant Human IL‑27 His-tag Biotinylated Protein (Catalog # BT2526) binds with an ED₅₀ of 4.00‑40.0 ng/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein (1479-TC) is immobilized at 0.500 μg/mL (100 μL/well), Recombinant Human IL‑27 His-tag Biotinylated Protein (Catalog # BT2526) binds with an ED₅₀ of 4.00‑40.0 ng/mL." class="big_thumb" />