HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm and cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm and cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="FRS2 was detected in immersion fixed paraffin-embedded sections of human normal liver and liver cancer tissue using Sheep Anti-Human FRS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4069) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm and cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="A172 human glioblastoma cell line was stained with Mouse Anti-Human FRS2 Monoclonal Antibody (Catalog # MAB40691, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />