CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="FKBP51 was detected in immersion fixed paraffin-embedded sections of human prostate using 15 µg/mL Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4094-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96873-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84676-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70742 | |||
HAF005). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP51 Monoclonal Antibody (Catalog # MAB4094) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4094-SP | |||
HAF109). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
HAF018). A specific band was detected for FKBP51 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 
![Immunohistochemistry: FKBP51/FKBP5 Antibody [NB100-68240] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FKBP5/FKBP51 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-NB100-68240-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - Western Blot and Immunoprecipitation: FKBP5/FKBP51 Antibody NB100-68240 - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68240 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. FKBP5/FKBP51 was also immunoprecipitated by rabbit anti-FKBP5/FKBP51 antibody NB100-68239, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-NB100-68240-img0002.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) & ABCB5 mRNA (grey) (n=3). FKBP51-treated sample expression=1. (Right) Western blot assay of ABCB5 & FKBP51 levels. (b) FKBP51 overexpression enhances ABCB5 levels. (Left) Normalized expression of FKBP51 mRNA (black) & ABCB5 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=3. (Left) Western blot assay of ABCB5 & FKBP51 levels in the same cells. Anti-Flag-labelled exogenous FKBP51. (c) ABCG2+ melanoma cells increase in FKBP51-overexpressing cells. (Upper) Normalized expression of FKBP51 mRNA (black) & ABCG2 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=5. (Lower) Flow cytometric histograms of ABCG2 expression (green population); mean±S.D. of counts are shown. (d) Enhanced FKBP51 mRNA levels in sorted ABCG2+ melanoma cells (SAN, upper; A375, lower). Whole cell expression=1; (n=3). (e) Enhanced EMT gene transcripts in sorted ABCG2+ melanoma cells. ABCG2− sample expression=1; n=3. (f) Expression of ABCG2 transcript in 9 deparaffinized tumours, 4 primary melanoma (M) samples, & 5 metastases (MM). A naevus sample was arbitrarily chosen with expression=1 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68240_rabbit-polyclonal-fkbp51-fkbp5-antibody-31020241612662.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) & ABCB5 mRNA (grey) (n=3). FKBP51-treated sample expression=1. (Right) Western blot assay of ABCB5 & FKBP51 levels. (b) FKBP51 overexpression enhances ABCB5 levels. (Left) Normalized expression of FKBP51 mRNA (black) & ABCB5 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=3. (Left) Western blot assay of ABCB5 & FKBP51 levels in the same cells. Anti-Flag-labelled exogenous FKBP51. (c) ABCG2+ melanoma cells increase in FKBP51-overexpressing cells. (Upper) Normalized expression of FKBP51 mRNA (black) & ABCG2 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=5. (Lower) Flow cytometric histograms of ABCG2 expression (green population); mean±S.D. of counts are shown. (d) Enhanced FKBP51 mRNA levels in sorted ABCG2+ melanoma cells (SAN, upper; A375, lower). Whole cell expression=1; (n=3). (e) Enhanced EMT gene transcripts in sorted ABCG2+ melanoma cells. ABCG2− sample expression=1; n=3. (f) Expression of ABCG2 transcript in 9 deparaffinized tumours, 4 primary melanoma (M) samples, & 5 metastases (MM). A naevus sample was arbitrarily chosen with expression=1 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68240_rabbit-polyclonal-fkbp51-fkbp5-antibody-310202415535195.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 interacts with the general transcriptional co-activator p300. (a, left) FKBP51 co-immunoprecipitates with p300. (a, right), p300 co-immunoprecipitates with FKBP51. Total-cell lysates were prepared by SAN melanoma cells transfected with FKBP51/Flag. Cell lysates were immunoprecipitated with anti-Kat3B/p300 (IP p300) or anti-Flag (IP FKBP51). Immunoprecipitated & total lysates were then subjected to western blot with anti-FKBP51 or anti-p300. (b) ChIP performed with SAN melanoma cells, silenced (FKBP51 siRNA) or not (NS RNA) for FKBP51. An enrichment of DNA (region at −3450 from TSS of ABCG2 gene) can be observed in p300-immunoprecipitated chromatin (NS RNA) compared with IgG sample. Such an enrichment appeared to be reduced when FKBP51 was silenced (FKBP51 siRNA) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68240_rabbit-polyclonal-fkbp51-fkbp5-antibody-310202415532018.jpg)
![Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP51/FKBP5 Monoclonal Antibody, Clone Hi51B (NB110-96873). Tissue: MK cells. Species: Mouse. Primary Antibody: Mouse Anti-FKBP51/FKBP5 Monoclonal Antibody (NB110-96873) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Cells stained red. Courtesy of: the Hospital Henri Mondor, France.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Hi51B-Immunocytochemistry-Immunofluorescence-NB110-96873-img0006.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - analysis of Human HeLa cell lysates showing detection of FKBP51 protein using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Hi51B-Western-Blot-NB110-96873-img0005.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - FKBP51/Hsp90 bind to GluR1-type AMPA receptors to regulate trafficking. A, Representative Western blottings form biotinylation assays of receptor endocytosis was performed on ex vivo slices, as described in Materials & Methods, rTgFKBP5 (N = 4; n = 8), WT (N = 2; n = 8), & tTA (N = 2; n = 8). Following labeling with Sulfo-NHS-SS biotin & chemical LTD (20 µM NMDA; 5 min) treatment, receptors were permitted to externalize at 30°C for the indicated times. B, The quantification ± SEM of multiple acquisitions is shown for GluR1. C, Representative Western blottings from anti-GluR1 co-immunoprecipitations & corresponding inputs from control & rTgFKBP5 mice immunoblotted as indicated. rTgFKBP5 (N = 2), WT (N = 2), & tTA (N = 2) total from two independent experiments. D, Representative Western blottings of anti-GluR1 co-immunoprecipitations & corresponding inputs from HEK293T cells transfected with GluR1 & FKBP51 or empty vector (EV) for 48 h were immunoblotted with antibodies as indicated. Just before harvest, cells were treated with 100 µM AMPA or PBS for 10 min to induce GluR1 receptor internalization. *p = 0.0286 by t-test of this time point. **p < 0.001 by two-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30963102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96873_mouse-monoclonal-fkbp51-fkbp5-antibody-hi51b-310202416205195.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - Detailed schematic & validation of the FKBP5 TRE transgene. A, To allow for site-directed, single copy insertion into the mouse genome in chromosome 11, the transgenic construct contained flanking attB sites via a PhiC31 integrase. The downstream Mp1 poly A tail will help maintain stable expression. To drive high expression, the transgenic construct included a tetracycline-response element (TRE) promoter made of seven repeats of the tetracycline operators used to drive high expression of the singly inserted FKBP5 gene in the presence of the tTA, & a weak minimal CMV promoter which produces low basal expression. B, Western blotting from HEK293T cells transfected with increasing amounts of FKBP5 TRE plasmid, as indicated, for 48 h. C, HEK293T cells were transfected with the indicated amounts of FKBP5 TRE & tTA plasmid, to ensure the tTA would drive high FKBP51 expression. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30963102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96873_mouse-monoclonal-fkbp51-fkbp5-antibody-hi51b-310202416203721.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - FKBP51 expression & distribution in rTgFKBP5 mice. A, Expression of human & mouse FKBP5 in rTgFKBP5 mice expressed as fold change ± SEM compared to WT mice using qPCR; ***p < 0.001 by t test (N = 10) with three technical replicates. B, Western blotting showing FKBP51 levels in the hippocampus from rTgFKBP5, WT, FKBP5, & tTA mice. C, Western blotting showing levels of FKBP51 levels in the rTgFKBP5 hippocampus from 1 to 10 µg of protein loaded compared to 50 µg of protein from WT or FKBP51 mice. GAPDH levels are shown to confirm protein load. See Extended Data Figure 2-1 for more information on the antibody. D, 20× images of anti-FKBP51 staining from rTgFKBP5 mice. The entorhinal cortex (ECX), anterior cortex (ACX), CA1, CA3, & dentate gyrus (DG) are labeled. E, 20× images of anti-FKBP51 staining from rTgFKBP5 mice in the CA1, CA3, DG, ECX, & ACX. Scale bar = 100 µm; 10 µm (inset). F, Western blotting showing FKBP51 levels in the hippocampus, striatum, ACX, posterior cortex (PCX), interbrain, thalamus & hypothalamus, & cerebellum of a rTgFKBP5 mouse. G, Quantitation of FKBP51 proteins levels throughout the hippocampus (HPC), striatum (STR), ACX, PCX, interbrain, thalamus & hypothalamus (INTER), & cerebellum (CER), of rTgFKBP5 mice from multiple exposures. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30963102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96873_mouse-monoclonal-fkbp51-fkbp5-antibody-hi51b-310202416212341.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - FKBP51/Hsp90 bind to GluR1-type AMPA receptors to regulate trafficking. A, Representative Western blottings form biotinylation assays of receptor endocytosis was performed on ex vivo slices, as described in Materials & Methods, rTgFKBP5 (N = 4; n = 8), WT (N = 2; n = 8), & tTA (N = 2; n = 8). Following labeling with Sulfo-NHS-SS biotin & chemical LTD (20 µM NMDA; 5 min) treatment, receptors were permitted to externalize at 30°C for the indicated times. B, The quantification ± SEM of multiple acquisitions is shown for GluR1. C, Representative Western blottings from anti-GluR1 co-immunoprecipitations & corresponding inputs from control & rTgFKBP5 mice immunoblotted as indicated. rTgFKBP5 (N = 2), WT (N = 2), & tTA (N = 2) total from two independent experiments. D, Representative Western blottings of anti-GluR1 co-immunoprecipitations & corresponding inputs from HEK293T cells transfected with GluR1 & FKBP51 or empty vector (EV) for 48 h were immunoblotted with antibodies as indicated. Just before harvest, cells were treated with 100 µM AMPA or PBS for 10 min to induce GluR1 receptor internalization. *p = 0.0286 by t-test of this time point. **p < 0.001 by two-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30963102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96873_mouse-monoclonal-fkbp51-fkbp5-antibody-hi51b-310202416212343.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (Hi51B) [NB110-96873] - Detailed schematic & validation of the FKBP5 TRE transgene. A, To allow for site-directed, single copy insertion into the mouse genome in chromosome 11, the transgenic construct contained flanking attB sites via a PhiC31 integrase. The downstream Mp1 poly A tail will help maintain stable expression. To drive high expression, the transgenic construct included a tetracycline-response element (TRE) promoter made of seven repeats of the tetracycline operators used to drive high expression of the singly inserted FKBP5 gene in the presence of the tTA, & a weak minimal CMV promoter which produces low basal expression. B, Western blotting from HEK293T cells transfected with increasing amounts of FKBP5 TRE plasmid, as indicated, for 48 h. C, HEK293T cells were transfected with the indicated amounts of FKBP5 TRE & tTA plasmid, to ensure the tTA would drive high FKBP51 expression. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30963102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96873_mouse-monoclonal-fkbp51-fkbp5-antibody-hi51b-310202416212350.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NBP1-84676] - Analysis using Anti-FKBP5 antibody NBP1-84676 (A) shows similar pattern to independent antibody NBP1-84677 (B).](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-NBP1-84676-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: FKBP51/FKBP5 Antibody [NBP1-84676] - Analysis using Anti-FKBP5 antibody NBP1-84676 (A) shows similar pattern to independent antibody NBP1-84677 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84676-img0020.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84676-img0014.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84676-img0017.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84676-img0019.jpg)
![Western Blot: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP5/FKBP51 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP5/FKBP51.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Western-Blot-NBP1-47755-img0001.jpg "Western Blot: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP5/FKBP51 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP5/FKBP51.")
![Immunocytochemistry/Immunofluorescence: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FKBP5/FKBP51.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Immunocytochemistry-Immunofluorescence-NBP1-47755-img0007.jpg)
![Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded prostate tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Immunohistochemistry-Paraffin-NBP1-47755-img0006.jpg)
![Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Immunohistochemistry-Paraffin-NBP1-47755-img0002.jpg)
![Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded colon tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Immunohistochemistry-Paraffin-NBP1-47755-img0003.jpg)
![Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded endometrium tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Immunohistochemistry-Paraffin-NBP1-47755-img0004.jpg)
![Immunohistochemistry-Paraffin: FKBP5/FKBP51 Antibody (3E9) [NBP1-47755] - Staining of paraffin-embedded pancreas tissue using anti-FKBP5/FKBP51 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP5-FKBP51-Antibody-3E9-Immunohistochemistry-Paraffin-NBP1-47755-img0005.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [H00002289-B01P] - Analysis of FKBP5 expression in human placenta.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-H00002289-B01P-img0003.jpg "Western Blot: FKBP51/FKBP5 Antibody [H00002289-B01P] - Analysis of FKBP5 expression in human placenta.")
![Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody [H00002289-B01P] - Analysis of purified antibody to FKBP5 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunocytochemistry-Immunofluorescence-H00002289-B01P-img0001.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [H00002289-B01P] - Analysis of purified antibody to FKBP5 on formalin-fixed paraffin-embedded human endometrium. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-H00002289-B01P-img0002.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [H00002289-B01P] - Analysis of FKBP5 expression in transfected 293T cell line by FKBP5 polyclonal antibody. Lane 1: FKBP5 transfected lysate(50.27 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-H00002289-B01P-img0004.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (7W2R1) [NBP3-16750] - Western blot analysis of extracts of various cell lines, using FKBP51/FKBP5 Rabbit mAb (NBP3-16750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-7W2R1-Western-Blot-NBP3-16750-img0003.jpg "Western Blot: FKBP51/FKBP5 Antibody (7W2R1) [NBP3-16750] - Western blot analysis of extracts of various cell lines, using FKBP51/FKBP5 Rabbit mAb (NBP3-16750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody (7W2R1) [NBP3-16750] - Immunohistochemistry of paraffin-embedded human thyroid cancer using FKBP51/FKBP5 Rabbit mAb (NBP3-16750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-7W2R1-Immunohistochemistry-Paraffin-NBP3-16750-img0002.jpg)
![Western Blot: FKBP51/FKBP5 Antibody (7W2R1) [NBP3-16750] - Western blot analysis of extracts of various cell lines, using FKBP51/FKBP5 Rabbit mAb (NBP3-16750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-7W2R1-Western-Blot-NBP3-16750-img0004.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody (7W2R1) [NBP3-16750] - Immunohistochemistry of paraffin-embedded rat ovary using FKBP51/FKBP5 Rabbit mAb (NBP3-16750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-7W2R1-Immunohistochemistry-Paraffin-NBP3-16750-img0001.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84677] - Staining in human breast and cerebral cortex tissues using anti-FKBP5 antibody. Corresponding FKBP5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84677-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84677] - Staining in human breast and cerebral cortex tissues using anti-FKBP5 antibody. Corresponding FKBP5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84677] - Staining of human breast shows high expression.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84677-img0007.jpg)
![Immunohistochemistry-Paraffin: FKBP51/FKBP5 Antibody [NBP1-84677] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-Paraffin-NBP1-84677-img0008.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 2. Western blot analysis using the antibody directed against mouse FKBP51/FKBP5 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse FKBP51/FKBP5 , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 51 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-NBP3-18673-img0001.jpg "Western Blot: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 2. Western blot analysis using the antibody directed against mouse FKBP51/FKBP5 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse FKBP51/FKBP5 , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 51 kDa) is indicated on the right.")
![ELISA: FKBP51/FKBP5 Antibody [NBP3-18673] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse FKBP51/FKBP5 . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,400.](http://images.novusbio.com/fullsize/FKBP51-FKBP5 Antibody-ELISA-NBP3-18673-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody (3D1-1B10) [H00002289-M02] - Analysis of monoclonal antibody to FKBP5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-3D1-1B10-Immunocytochemistry-Immunofluorescence-H00002289-M02-img0004.jpg "Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody (3D1-1B10) [H00002289-M02] - Analysis of monoclonal antibody to FKBP5 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: FKBP51/FKBP5 Antibody (3D1-1B10) [H00002289-M02] - Detection limit for recombinant GST tagged FKBP5 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-3D1-1B10-ELISA-H00002289-M02-img0006.jpg)
![SDS-Page: Recombinant Human FKBP51/FKBP5 Protein [H00002289-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002289-P01-1.jpg "SDS-Page: Recombinant Human FKBP51/FKBP5 Protein [H00002289-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
