CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="DcR3/TNFRSF6B was detected in immersion fixed paraffin-embedded sections of human stomach cancer using 10 µg/mL Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox"> MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />