Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED₅₀ for this effect is 0.03‑0.15 µg/mL in the presence of 20 ng/mL rhFas Ligand.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DcR3/TNFRSF6B protein
  

  Human DcR3 (Val 24-His300) Accession # O95407	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O95407
• N-terminal Sequence: Val24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: TNFRSF6B
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

• IAP Inhibitors

Background

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

1. Pitti, R.M. et al. (1998) Nature 396:699.
2. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
3. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.

Publications for IAP Inhibitors (142-DC)(11)

Publications using 142-DC

• A Zwolak, SR Chan, P Harvilla, S Mahady, AA Armstrong, L Luistro, N Tamot, D Yamada, M Derebe, S Pomerantz, M Chiu, R Ganesan, P Chowdhury A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3 Scientific Reports, 2022-11-29;12(1):20538. 2022-11-29 [PMID: 36446890] (Bioassay, Human)
• YJ Peng, CT Peng, YH Lin, GJ Lin, SH Huang, SJ Chen, HK Sytwu, CP Cheng Decoy Receptor 3 Promotes Preosteoclast Cell Death via Reactive Oxygen Species-Induced Fas Ligand Expression and the IL-1alpha/IL-1 Receptor Antagonist Pathway Mediators Inflamm., 2020-06-10;2020(0):1237281. 2020-06-10 [PMID: 32587467] (Bioassay, Mouse)
• M Idelson, R Alper, A Obolensky, N Yachimovic, J Rachmilewi, A Ejzenberg, E Beider, E Banin, B Reubinoff Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Stem Cell Reports, 2018-08-16;0(0):. 2018-08-16 [PMID: 30122442] (Bioassay, Human)
• AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-03-05;0(0):1-43. 2018-03-05 [PMID: 29436901] (ELISA Capture, Human)
• DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1. BMC Cancer, 2012-05-14;12(0):176. 2012-05-14 [PMID: 22583667] (Flow Cytometry, Human)
• Tso GH, Law HK, Tu W Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Stem Cells, 2010-05-01;28(5):939-54. 2010-05-01 [PMID: 20222014] (Bioassay, Human)
• Morishige T, Yoshioka Y, Inakura H, Tanabe A, Yao X, Tsunoda S, Tsutsumi Y, Mukai Y, Okada N, Nakagawa S Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy. Biomaterials, 2010-02-01;31(12):3357-63. 2010-02-01 [PMID: 20117833] (Surface Plasmon Resonance)
• Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients. Leukemia, 2009-07-09;23(11):2139-46. 2009-07-09 [PMID: 19587706] (Bioassay, Human)
• Mueller AM, Pedre X, Killian S, David M, Steinbrecher A The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid. J. Neuroimmunol., 2009-03-06;209(1):57-64. 2009-03-06 [PMID: 19269042] (Bioassay, Mouse)
• Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts. J. Immunol., 2007-01-01;178(1):192-200. 2007-01-01 [PMID: 17182555] (Bioassay, Mouse)
• Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum., 2006-05-01;54(5):1451-62. 2006-05-01 [PMID: 16649193] (Bioassay, Human)

Bioinformatics

• Gene Symbol: TNFRSF6B
• Uniprot:
  • O95407()