HAF016). Specific bands were detected for COMT at approximately 25 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human placenta tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human COMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for COMT at approximately 25 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7386-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26646-50ul | |||
![Western Blot: COMT Antibody [NBP2-15954] - Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NBP2-15954-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COMT Antibody [NBP2-15954] - Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: COMT Antibody [NBP2-15954] - COMT antibody detects COMT protein by Western blot analysis. A. 30 ug K562 whole cell lysate/extract 12 % SDS-PAGECOMT antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NBP2-15954-img0001.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP2-15954-img0002.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Paraffin-embedded Rat brain. COMT antibody diluted at 1:500.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NBP2-15954-img0003.jpg)
![Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-1780-img0002.jpg "Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: COMT Antibody [NB100-1780] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/COMT-Antibody-Flow-Cytometry-NB100-1780-img0003.jpg)
![Western Blot: COMT Antibody [NB100-1780] - Staining of Human Liver (A) with antibody at 2 ug/mL and Ovary (B) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-1780-img0001.jpg)
![Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-NB100-41387-img0007.jpg "Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0006.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Cerebellum. Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0004.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.](http://images.novusbio.com/fullsize/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0005.jpg)
![Western Blot: COMT Antibody (1G4-1A1) [H00001312-M01] - Analysis of COMT expression in transfected 293T cell line by COMT monoclonal antibody (M01), clone 1G4-1A1.Lane 1: COMT transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COMT-Antibody-1G4-1A1-Western-Blot-H00001312-M01-img0005.jpg "Western Blot: COMT Antibody (1G4-1A1) [H00001312-M01] - Analysis of COMT expression in transfected 293T cell line by COMT monoclonal antibody (M01), clone 1G4-1A1.Lane 1: COMT transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: COMT Antibody (1G4-1A1) [H00001312-M01] - COMT monoclonal antibody (M01), clone 1G4-1A1 Analysis of COMT expression in A-431.](http://images.novusbio.com/fullsize/COMT-Antibody-1G4-1A1-Western-Blot-H00001312-M01-img0004.jpg)
![Sandwich ELISA: COMT Antibody (1G4-1A1) [H00001312-M01] - Detection limit for recombinant GST tagged COMT is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/COMT-Antibody-1G4-1A1-Sandwich-ELISA-H00001312-M01-img0003.jpg)
![Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] - Image of COMT Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26646_rabbit-comt-mab-8e1-262202415355741.jpg "Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] - Image of COMT Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: COMT Antibody (8E1) [NBP3-26646] - Positive Western Blot detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate. All lanes: COMT Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 31, 25 kDa Observed band size: 31 kDa](http://images.novusbio.com/fullsize/nbp3-26646_rabbit-comt-mab-8e1-282202411363016.jpg)
![Western Blot: COMT Antibody (9Y2A3) [NBP3-16413] - Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (NBP3-16413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/COMT-Antibody-9Y2A3-Western-Blot-NBP3-16413-img0001.jpg "Western Blot: COMT Antibody (9Y2A3) [NBP3-16413] - Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (NBP3-16413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: COMT Antibody (9Y2A3) [NBP3-16413] - Western blot analysis of extracts of various cell lines, using COMT Rabbit mAb (NBP3-16413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COMT-Antibody-9Y2A3-Western-Blot-NBP3-16413-img0002.jpg)
![Western Blot: COMT Antibody [H00001312-B01P] - Analysis of COMT expression in human placenta.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-H00001312-B01P-img0004.jpg "Western Blot: COMT Antibody [H00001312-B01P] - Analysis of COMT expression in human placenta.")
![Western Blot: COMT Antibody [H00001312-B01P] - Analysis of COMT expression in transfected 293T cell line by COMT polyclonal antibody. Lane 1: COMT transfected lysate(24.31 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COMT-Antibody-Western-Blot-H00001312-B01P-img0005.jpg)
![ELISA: Human COMT ELISA Kit (Colorimetric) [NBP3-27406] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27406_human-comt-elisa-kit-colorimetric-114202410262137.png "ELISA: Human COMT ELISA Kit (Colorimetric) [NBP3-27406] - Standard Curve Reference")
