| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-104SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7446-SP | |||
| Carrier Free | FAB7446V-100UG | |||
| Carrier Free | FAB7446U-100UG | |||
| Carrier Free | FAB7446T-100UG | |||
| Carrier Free | FAB7446S-100UG | |||
| Carrier Free | FAB7446R-100UG | |||
| Carrier Free | FAB7446N-100UG | |||
| Carrier Free | FAB7446G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65315-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34714-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7446 | |||
| Carrier Free | MAB7446-SP | |||
| Carrier Free | FAB7446V-100UG | |||
| Carrier Free | FAB7446U-100UG | |||
| Carrier Free | FAB7446T-100UG | |||
| Carrier Free | FAB7446S-100UG | |||
| Carrier Free | FAB7446R-100UG | |||
| Carrier Free | FAB7446N-100UG | |||
| Carrier Free | FAB7446G-100UG | |||
![Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Simple-Western-NB400-104-img0014.jpg "Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% BSA. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-104-img0030.jpg)
![Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Cytometry-NB400-104-img0026.jpg)
![Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0013.jpg)
![Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-NB400-104-img0011.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane signal of SR-BI protein in the murine hepatocytes.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0022.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SR-BI Antibody [NB400-104] - HCV replication was increased by CD36. Silence of CD36 decreased HCV infection and replication (n = 3). Huh7.5 cells were transfected with 0.2 u g CD36-HA plasmid or with 150 pmol siRNA for CD36 (B) for 48 hrs, and then were washed and infected with HCV (150 IU/cell) for 2 hrs, followed by washing and continuously incubating. Intracellular HCV RNA and proteins were detected in 96 hrs, and the cytotoxicity of siRNAs was measured in 96-wells plate with a MTT assay in B. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21808) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0034.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-104-img0029.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated mainly a membranous signal of SR-BI protein in the murine hepatocytes.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0023.jpg)
![Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Cytometry-NB400-104-img0004.jpg)
![Western Blot: SR-BI Antibody - BSA Free [NB400-104] - Effects of 13-HODE & LA in the presence & absence of PPAR alpha & PPAR gamma selective antagonists on molecular markers of cholesterol homeostasis in RAW264.7 macrophages. RAW264.7 cells were pre-treated without or with the PPAR alpha selective antagonist GW6471 or the PPAR gamma selective antagonist GW9662 & subsequently treated without (vehicle control) or with 2.5 μmol/L 13-HODE or 100 μmol/L LA for 24 h. Afterwards, cells were lysed & subsequently processed for western blotting as described in the materials & methods section. A, Representative immunoblots specific for ABCA1, ABCG1, SR-BI, LXR alpha , & beta -actin which was used for normalization are shown. B, Bars represent data from densitometric analysis & are means ± SD from three independent experiments (n = 3). Data are expressed as percentage of protein concentration of vehicle control cells. Results from statistical analysis are indicated: Significant effects are denoted with superscript letters. Bars marked without a common superscript letter significantly differ (P  2000) (g, h, i, respectively). Bar graph shows the mean percentage of positively stained cells. Error bars depict standard deviations. ****p  0.3) by one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29523748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-104_rabbit-polyclonal-sr-bi-antibody-31020241618928.jpg)
![Western Blot: SR-BI Antibody - BSA Free [NB400-104] - Differentiation & characteristics of iHLCs. (a) Cell morphology & culture conditions at different stages of differentiation from iPSCs into iHLCs. Bright-field images were taken at the indicated time points (scale bar 100 µm). (b) Sequential expression & repression of transcription factors marking successful differentiation. Cells were immunostained with antibodies against Oct 3/4 (pluripotency marker), the endodermal transcription factor GATA-4, & the hepatic marker HNF4 alpha at day 0, 5, & 10 of differentiation (scale bars 20 µm). Shown are representative fluorescence & DIC images. (c) mRNA expression levels of different hepatocyte-specific factors in iHLCs, Huh7.5 cells, & primary human hepatocytes (PHHs) were analysed via qRT-PCR. Expression levels are displayed as fold over iPSCs normalised to GAPDH & 18S rRNA (mean ± SEM, n = 3–4, *p  0.9) by one-way ANOVA. §P < 0.02 by unpaired Student's t-test, relative to 0 h time point. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29523748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-104_rabbit-polyclonal-sr-bi-antibody-31020241616010.jpg)
![Immunohistochemistry: CD1b Antibody (CC20) [NB100-65315] - Detection of Mouse anti Bovine CD1w2 in an analysis on acetone-fixed frozen bovine thymus using the Histar detection system, original magnification x60.](http://images.novusbio.com/fullsize/CD1b-Antibody-CC20-Immunohistochemistry-NB100-65315-img0005.jpg "Immunohistochemistry: CD1b Antibody (CC20) [NB100-65315] - Detection of Mouse anti Bovine CD1w2 in an analysis on acetone-fixed frozen bovine thymus using the Histar detection system, original magnification x60.")
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and Mouse IgG2a isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and Mouse anti Bovine CD1w2 Antibody conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody.](http://images.novusbio.com/fullsize/CD1b-Antibody-CC20-Flow-Cytometry-NB100-65315-img0004.jpg)
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody. All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes.](http://images.novusbio.com/fullsize/CD1b-Antibody-CC20-Flow-Cytometry-NB100-65315-img0001.jpg)
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody and Mouse IgG2a isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody. Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody and Mouse anti Bovine CD1w2 Antibody conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody.](http://images.novusbio.com/fullsize/CD1b-Antibody-CC20-Flow-Cytometry-NB100-65315-img0002.jpg)
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody.](http://images.novusbio.com/fullsize/CD1b-Antibody-CC20-Flow-Cytometry-NB100-65315-img0003.jpg)
![Immunohistochemistry-Paraffin: CD1b Antibody [NBP1-86559] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/CD1b-Antibody-Immunohistochemistry-Paraffin-NBP1-86559-img0002.jpg "Immunohistochemistry-Paraffin: CD1b Antibody [NBP1-86559] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.")
![Flow Cytometry: CD1b Antibody (RIV12) - Azide and BSA Free [NBP2-34714] - FCM staining of human PBMCs using CD1b MAb (RIV12).](http://images.novusbio.com/fullsize/CD1b-Antibody-RIV12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34714-img0001.jpg "Flow Cytometry: CD1b Antibody (RIV12) - Azide and BSA Free [NBP2-34714] - FCM staining of human PBMCs using CD1b MAb (RIV12).")
![Flow Cytometry: CD1b Antibody (RIV12) [NBP2-32925] - FCM staining of human PBMCs using CD1b MAb (RIV12).](http://images.novusbio.com/fullsize/CD1b-Antibody-RIV12-Flow-Cytometry-NBP2-32925-img0001.jpg "Flow Cytometry: CD1b Antibody (RIV12) [NBP2-32925] - FCM staining of human PBMCs using CD1b MAb (RIV12).")
![Western Blot: CD1b Antibody [NBP1-33254] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CD1b-Antibody-Western-Blot-NBP1-33254-img0002.jpg "Western Blot: CD1b Antibody [NBP1-33254] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: CD1b Overexpression Lysate (Adult Normal) [NBP2-08177] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD1b.](http://images.novusbio.com/fullsize/CD1b-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08177-img0001.jpg "Western Blot: CD1b Overexpression Lysate (Adult Normal) [NBP2-08177] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD1b.")
![SDS-Page: Recombinant Human CD1b Protein [NBP2-22744]](http://images.novusbio.com/fullsize/Recombinant-Human-CD1b-Protein-SDS-Page-NBP2-22744-img0002.jpg "SDS-Page: Recombinant Human CD1b Protein [NBP2-22744]")
![Sandwich ELISA: CD1b Antibody (2E4) [H00000910-M06] - Detection limit for recombinant GST tagged CD1B is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CD1b-Antibody-2E4-Sandwich-ELISA-H00000910-M06-img0001.jpg "Sandwich ELISA: CD1b Antibody (2E4) [H00000910-M06] - Detection limit for recombinant GST tagged CD1B is 0.3 ng/ml as a capture antibody.")