| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44300SS | |||
| Azide and BSA Free | NBP2-44301SS | |||
| Azide and BSA Free | NBP2-44301 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44302SS | |||
| Azide and BSA Free | NBP2-44304SS | |||
| Azide and BSA Free | NBP2-44304 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85325-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55086-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09015-0.025mg | |||
![Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Western-Blot-NBP2-44300-img0004.jpg "Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.")
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (5C9NB) [NBP2-44300] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (5C9NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Immunocytochemistry-Immunofluorescence-NBP2-44300-img0005.jpg)
![Immunohistochemistry: HLA DMA Antibody (5C9NB) [NBP2-44300] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 5C9NB). The antibody generated a diffused to punctate staining in cellular membranes and cytoplasm, especially in the perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Immunohistochemistry-NBP2-44300-img0003.jpg)
![Flow (Cell Surface): HLA DMA Antibody (5C9NB) [NBP2-44300] - Analysis using the Azide Free version of NBP2-44300. Detection of Human PBMC cells (surface) with HLA DMA (5C9NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Flow-Cell-Surface-NBP2-44300-img0007.jpg)
![Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Western-Blot-NBP2-44302-img0005.jpg "Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)")
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (6B12NB) [NBP2-44302] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (6B12NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Immunocytochemistry-Immunofluorescence-NBP2-44302-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) [NBP2-44302] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 6B12NB). The antibody generated a punctate staining in the cytoplasm/perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Immunohistochemistry-Paraffin-NBP2-44302-img0003.jpg)
![Flow (Cell Surface): HLA DMA Antibody (6B12NB) [NBP2-44302] - Analysis using the Azide Free version of NBP2-44302. Detection of Human PBMC cells (surface) with HLA DMA (6B12NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Flow-Cell-Surface-NBP2-44302-img0008.jpg)
![Western Blot: HLA DMA Antibody [NBP1-85325] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DMA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Western-Blot-NBP1-85325-img0009.jpg "Western Blot: HLA DMA Antibody [NBP1-85325] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DMA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining in human tonsil and cerebral cortex tissues using anti-HLA-DMA antibody. Corresponding HLA-DMA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunohistochemistry-Paraffin-NBP1-85325-img0008.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunohistochemistry-Paraffin-NBP1-85325-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunohistochemistry-Paraffin-NBP1-85325-img0006.jpg)
![Western Blot: HLA DMA Antibody (3F12-F11) [H00003108-M01] - HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Analysis of HLA-DMA expression in U-2 OS.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-Western-Blot-H00003108-M01-img0009.jpg "Western Blot: HLA DMA Antibody (3F12-F11) [H00003108-M01] - HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Analysis of HLA-DMA expression in U-2 OS.")
![Immunohistochemistry-Paraffin: HLA DMA Antibody (3F12-F11) [H00003108-M01] - Analysis of monoclonal antibody to HLA-DMA on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-Immunohistochemistry-Paraffin-H00003108-M01-img0007.jpg)
![ELISA: HLA DMA Antibody (3F12-F11) [H00003108-M01] - Detection limit for recombinant GST tagged HLA-DMA is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-ELISA-H00003108-M01-img0004.jpg)
![ELISA: HLA DMA Antibody (3F12-F11) [H00003108-M01] - Detection limit for recombinant GST tagged HLA-DMA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-ELISA-H00003108-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody [NBP2-55086] - Staining of human cell line RT4 shows localization to vesicles.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55086-img0001.jpg "Immunocytochemistry/Immunofluorescence: HLA DMA Antibody [NBP2-55086] - Staining of human cell line RT4 shows localization to vesicles.")
![Western Blot: HLA DMA Overexpression Lysate (Adult Normal) [NBL1-11591] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HLA DMA.](http://images.novusbio.com/fullsize/HLA-DMA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11591-img0002.jpg "Western Blot: HLA DMA Overexpression Lysate (Adult Normal) [NBL1-11591] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HLA DMA.")
![Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-7420252226526.png "Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or NBP3-43539(B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522141218.png)
![Western Blot: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Rat spleen (A), rat thymus (B) and rat lymph node (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-43539 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD74 is ~24.5-31.6kDa, depending on the isoform and post-translational modification. NBP3-43539 successfully detected CD74 in rat spleen, thymus and lymph node lysates.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522113030.png)
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09015-img0002.jpg "Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of P7/7 (NBP3-09015, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Flow-Cytometry-NBP3-09015-img0001.jpg)