| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-51900 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 649 | NBP1-51902 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-51902 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP1-96825 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP1-96825 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72631 | |||
| Biotin | NBP1-73508 | |||
| FITC | NBP1-73280 | |||
| HRP | NBP1-73572 | |||
| Rhodamine | NBP1-73278 | |||
| Texas Red | NBP1-73509 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-72649 | |||
| Pre-adsorbed | NBP1-73191 | |||
| Pre-adsorbed | NBP1-73323 | |||
| Pre-adsorbed | NBP1-73579 | |||
| Pre-adsorbed | NBP1-73645 | |||
| Pre-adsorbed | NBP1-73695 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-73888-1mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB120-6111 | |||
| Rhodamine | NB120-6110 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB120-6111 | |||
| Pre-adsorbed | NB120-6110 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97198 | |||
| HRP | NBP1-97223 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97198 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB120-5888 | |||
| Biotin | NB120-5886 | |||
| Rhodamine | NB120-5885 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB120-5885 | |||
| Pre-adsorbed | NB120-5886 | |||
| Pre-adsorbed | NB120-5888 | |||
| Pre-adsorbed | NB120-5882 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72607 | |||
| Biotin | NBP1-73402 | |||
| FITC | NBP1-73209 | |||
| Rhodamine | NBP1-73208 | |||
| Texas Red | NBP1-73403 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72652 | |||
| FITC | NBP1-73496 | |||
| HRP | NBP1-73690 | |||
| Rhodamine | NBP1-73494 | |||
![Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Western-Blot-NB7588-img0006.jpg "Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7588-img0002.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of paraffin embedded human colonic tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0005.jpg)
![Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Flow-Cytometry-NB7588-img0004.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgG Secondary Antibody (Pre-absorbed) Conjugate at 1ug/mL blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the Cy5.5 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0008.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0007.jpg)
![Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed) [NBP1-51902] - Human cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Human-IgG-H+L-Secondary-Antibody-DyLight-649-Pre-adsorbed-Flow-Cytometry-NBP1-51902-img0001.jpg "Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed) [NBP1-51902] - Human cancer cell line. Image from verified customer review.")
![Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900] - Human cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Human-IgG-F-ab-2-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-51900-img0001.jpg "Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900] - Human cancer cell line. Image from verified customer review.")
![Dot Blot: Human IgG F(ab)2 Isotype Control [Biotin] [NBP1-96827] - Dot Blot results of Human IgG F(ab)2 isotype control [Biotin].Antigen: Human IgG F(ab)2 isotype control [Biotin].Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng.Blocking Buffer for 30 minutes at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 min at RT. Imaging System ChemiDoc, Filter used: Chemi.](http://images.novusbio.com/fullsize/Human-IgG-F-ab-2-Isotype-Control-Biotin-Dot-Blot-NBP1-96827-img0001.jpg "Dot Blot: Human IgG F(ab)2 Isotype Control [Biotin] [NBP1-96827] - Dot Blot results of Human IgG F(ab)2 isotype control [Biotin].Antigen: Human IgG F(ab)2 isotype control [Biotin].Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng.Blocking Buffer for 30 minutes at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 min at RT. Imaging System ChemiDoc, Filter used: Chemi.")
![Dot Blot results of Human IgG F(ab)2 Isotype Control [Biotin]. Antigen: Human IgG F(ab)2 Isotype Control [Biotin]. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. blocking Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP](http://images.novusbio.com/fullsize/nbp1-96827_human-human-igg-f-ab-2-isotype-control-biotin-23520231750164.jpg)
![Western Blot: Rabbit anti-Chicken IgG F(ab)2 Secondary Antibody [Unconjugated] [NBP1-72800] - Lane 1: Chicken IgG whole molecule (NBP1-97037). Lane 2: Chicken IgG F(c) Fragment (NBP1-96786). Lane 3: Chicken IgG F(ab) Fragment (NBP1-96773). All samples were reduced. Load: 50 ng per lane; incubated with blocking buffer for 30 min at RT. Primary Antibody: NBP1-72800 incubated for 60 min at RT. Secondary antibody: Goat anti-Rabbit IgG, peroxidase Conjugated antibody incubated in blocking buffer for 30 min at RT. Predicted/observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Chicken-IgG-F-ab-2-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72800-img0003.jpg "Western Blot: Rabbit anti-Chicken IgG F(ab)2 Secondary Antibody [Unconjugated] [NBP1-72800] - Lane 1: Chicken IgG whole molecule (NBP1-97037). Lane 2: Chicken IgG F(c) Fragment (NBP1-96786). Lane 3: Chicken IgG F(ab) Fragment (NBP1-96773). All samples were reduced. Load: 50 ng per lane; incubated with blocking buffer for 30 min at RT. Primary Antibody: NBP1-72800 incubated for 60 min at RT. Secondary antibody: Goat anti-Rabbit IgG, peroxidase Conjugated antibody incubated in blocking buffer for 30 min at RT. Predicted/observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.")
![Dot Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [NBP1-72800] - Chicken IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Chicken-IgG-F-ab-2-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72800-img0004.jpg)
![Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG, F(ab')2 secondary antibody (Catalog# NBP1-73640). Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Western-Blot-NBP1-72760-img0001.jpg "Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG, F(ab')2 secondary antibody (Catalog# NBP1-73640). Image from verified customer review. Image using the HRP form of this antibody.")
![ELISA: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760] - ELISA Results of Rabbit anti-Mouse IgG F(ab')2 Secondary antibody tested against purified Mouse IgG F(ab')2. Each well was coated in duplicate with 10 ug of Mouse IgG F(ab')2. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-ELISA-NBP1-72760-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG F(ab')2 Secondary Antibody [NBP2-60659] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab')2 (GOAT) Antibody 1:10,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Rabbit-IgG-Secondary-Antibody-Unconjugated-Western-Blot-NBP2-60659-img0002.jpg "Western Blot: Goat anti-Rabbit IgG F(ab')2 Secondary Antibody [NBP2-60659] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab')2 (GOAT) Antibody 1:10,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image using the HRP form of this antibody.")
![Western Blot: Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-6107] - Lane 1: Rabbit IgG F(ab')2 Load: 100 ng per lane Primary antibody: F(ab')2 Rabbit IgG F(ab')2 Antibody Pre-Adsorbed at 1:1000 o/n at 4C Secondary antibody: DyLight 800 Donkey anti-goat at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT Predicted/Observed size: 28 kDa, 28 kDa Other band(s): antigen breakdown](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Rabbit-IgG-Secondary-Antibody-Western-Blot-NB120-6107-img0003.jpg "Western Blot: Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-6107] - Lane 1: Rabbit IgG F(ab')2 Load: 100 ng per lane Primary antibody: F(ab')2 Rabbit IgG F(ab')2 Antibody Pre-Adsorbed at 1:1000 o/n at 4C Secondary antibody: DyLight 800 Donkey anti-goat at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT Predicted/Observed size: 28 kDa, 28 kDa Other band(s): antigen breakdown")
![SDS-Page: Mouse IgG F(ab)2 Isotype Control [NBP1-97018] - SDS-PAGE of Mouse IgG F(ab')2 Fragment. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab')2 Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Mouse-IgG-F-ab-2-Isotype-Control-SDS-Page-NBP1-97018-img0002.jpg "SDS-Page: Mouse IgG F(ab)2 Isotype Control [NBP1-97018] - SDS-PAGE of Mouse IgG F(ab')2 Fragment. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab')2 Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![Western Blot: Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody [PE] (Pre-adsorbed) [NB120-5889] - Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Mouse IgG F(ab')2. Other band(s): None.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Mouse-IgG-Secondary-Antibody-PE-Western-Blot-NB120-5889-img0004.jpg "Western Blot: Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody [PE] (Pre-adsorbed) [NB120-5889] - Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Mouse IgG F(ab')2. Other band(s): None.")
![Dot Blot: Goat anti-Bovine IgG F(ab')2 Secondary Antibody [NBP1-72717] - Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Bovine-IgG-F-ab-2-Secondary-Antibody-Pre-absorbed-Dot-Blot-NBP1-72717-img0001.jpg "Dot Blot: Goat anti-Bovine IgG F(ab')2 Secondary Antibody [NBP1-72717] - Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image from the FITC version of this antibody.")
![ELISA: Rabbit anti-Porcine IgG F(ab')2 Secondary Antibody [NBP1-72806] - ELISA Results of Rabbit anti-Porcine IgG F(ab')2 Secondary antibody tested against purified Swine IgG F(ab')2. Each well was coated in duplicate with 1.0 ug of Swine IgG F(ab')2 [Green Line] and Swine IgG [Blue Line]. The working dilution of Swine IgG F(ab')2 is 1:88,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Porcine-IgG-F-ab-2-Secondary-Antibody-ELISA-NBP1-72806-img0001.jpg "ELISA: Rabbit anti-Porcine IgG F(ab')2 Secondary Antibody [NBP1-72806] - ELISA Results of Rabbit anti-Porcine IgG F(ab')2 Secondary antibody tested against purified Swine IgG F(ab')2. Each well was coated in duplicate with 1.0 ug of Swine IgG F(ab')2 [Green Line] and Swine IgG [Blue Line]. The working dilution of Swine IgG F(ab')2 is 1:88,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.")