Goat anti-Bovine IgG F(ab)2 Secondary Antibody [Alkaline Phosphatase] (Pre-absorbed)

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Alkaline Phosphatase
Format
Pre-absorbed
Concentration
1 mg/ml

Order Details

Goat anti-Bovine IgG F(ab)2 Secondary Antibody [Alkaline Phosphatase] (Pre-absorbed) Summary

Immunogen
Bovine IgG F(ab')2 fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Bovine IgG, Bovine IgG F(ab)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Frozen 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2500 is suggested for this product.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, with 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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