Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP]

Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP] [NBP1-73640] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG, F(ab')2 secondary antibody (Catalog# NBP1-73640). Image from verified customer review.

• Reactivity: Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP
• Concentration: LYOPH

Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum
• Immunogen: Mouse IgG F(ab')2 fragment
• Specificity: No reaction was observed against Mouse IgG F(c).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Western Blot 1:1000 - 1:10000
• Application Notes: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Review for IgG F(ab')2 Antibody (NBP1-73640) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/08/2015

Summary

• Application: Western Blot
• Sample Tested: human cancer cell lysate
• Species: Human
• Lot: 12112

FAQs for IgG F(ab')2 Antibody (NBP1-73640). (Showing 1 - 1 of 1 FAQ).

1. I have couple of questions for you about your NBP1-73640 antibody. What exactly is this antibody? Is this antibody a Fab2 fragment only? And the data sheet says that Mouse IgG F(ab')2 fragment was used as an immunogen. When using Mouse IgG F(ab')2 fragment as an immunogen what does the antibody recognize versus when using Mouse IgG F(c) fragment as an immunogen? Are there some differences? I would like to have anti-Mouse IgG antibody that is IgG F(ab')2 fragment conjugated with HRP.
   • NBP1-73640 is affinity purified whole rabbit IgG which binds to the F(ab)2 part of mouse IgG. IgG normally is composed of two longer heavy chains with two shorter light chains in a Y shape. The arms of the Y are composed of both heavy and light chains and are the F(ab)2 or Fab fragments. The stem of the Y is the F(C) portion formed from the lower part of the heavy chains.