Goat anti-Human IgG F(ab')2 Secondary Antibody [HRP]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: 0.5 mg/ml

Goat anti-Human IgG F(ab')2 Secondary Antibody [HRP] Summary

• Immunogen: Human IgG F(ab')2 fragments
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000- 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
  • Western Blot 1:200-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.05% Pro-Clean 400
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Antiserum was solid phase adsorbed to ensure specificity. The antibody to human F(ab')2 was isolatedby affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generatedusing a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species. Suggested starting dilutions are: ELISA Colorimetric: 1:2000 - 1:20000 , Chemiluminescent: 1:5000 - 1:25000

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.