| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76570-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92814-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16891-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18335-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15485-20ul | |||
![Western Blot: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP1-76570-img0001.jpg "Western Blot: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL")
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody - BSA Free [NBP1-76570] - Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76570_rabbit-polyclonal-rock2-antibody-271120242074683.jpg)
![Immunohistochemistry: ROCK2 Antibody - BSA Free [NBP1-76570] - Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76570_rabbit-polyclonal-rock2-antibody-2711202420324878.jpg)
![Western Blot: PPP2R5A Antibody [NB100-847] - Staining of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.5 ug/mL. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.](http://images.novusbio.com/fullsize/PPP2R5A-Antibody-Western-Blot-NB100-847-img0002.jpg "Western Blot: PPP2R5A Antibody [NB100-847] - Staining of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.5 ug/mL. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PPP2R5A-Antibody-Immunohistochemistry-Paraffin-NB100-847-img0004.jpg)
![Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PPP2R5A-Antibody-Immunohistochemistry-Paraffin-NB100-847-img0003.jpg)
![Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-42202514251823.jpg%3Ft=1690982332 "Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of L929 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-42202514275299.jpg%3Ft=1690982383)
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of U2OS cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-422025143047.jpg%3Ft=1690982390)
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of H9C2 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-422025143045.jpg%3Ft=1690982372)
![Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP3-16891-img0001.jpg "Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Rat testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125711..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Mouse testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125830..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Human kidney tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125949..jpg)
![Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Western-Blot-NBP3-18335-img0001.jpg "Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Mouse Lung.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18335_rabbit-frizzled-6-pab-2711202419293843.jpg)
![Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Rat Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18335_rabbit-frizzled-6-pab-271120241958444.jpg)
![Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18335_rabbit-frizzled-6-pab-271120241956217.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Western blot analysis of extracts of various cell lines, using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-9T5J10-Western-Blot-NBP3-16264-img0006.jpg "Western Blot: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Western blot analysis of extracts of various cell lines, using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Immunofluorescence analysis of U-2 OS cells using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-9T5J10-Immunocytochemistry-Immunofluorescence-NBP3-16264-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Immunofluorescence analysis of NIH-3T3 cells using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-9T5J10-Immunocytochemistry-Immunofluorescence-NBP3-16264-img0004.jpg)
![Western Blot: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Western blot analysis of extracts of various cell lines, using CaM Kinase II delta Rabbit mAb (NBP3-16783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-10M1P2-Western-Blot-NBP3-16783-img0003.jpg "Western Blot: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Western blot analysis of extracts of various cell lines, using CaM Kinase II delta Rabbit mAb (NBP3-16783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Immunofluorescence analysis of NIH-3T3 cells using CaM Kinase II delta Rabbit mAb (NBP3-16783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-10M1P2-Immunocytochemistry-Immunofluorescence-NBP3-16783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Immunofluorescence analysis of C6 cells using CaM Kinase II delta Rabbit mAb (NBP3-16783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-delta-Antibody-10M1P2-Immunocytochemistry-Immunofluorescence-NBP3-16783-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Confocal imaging of NIH/3T3 cells using CaM Kinase II delta Rabbit mAb ollowed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16783_rabbit-cam-kinase-ii-delta-mab-10m1p2-52202511402754.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15485-img0003.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NBP3-15485-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RhoA-Antibody-Knockout-Validated-NBP3-15485-img0001.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0005.jpg "Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Analysis of anti-FZD9 / Frizzled 9 antibody with brains, neurons and glia at 14 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Analysis of anti-FZD9 / Frizzled 9 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0003.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0006.jpg)
![Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-278202415212459.jpg "Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-298202414333615.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-2920241023422.jpg)
![Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-288202412461668.jpg "Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32914) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-298202414262644.jpg)
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunocytochemistry analysis of HeLa cells labeling ROCK2 with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-29202410334061.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)